Protective effects of Salvia officinalis extract against cyclophosphamide-induced genotoxicity and oxidative stress in rats

Medicinal plants, especially rich in polyphenolic compounds, have been suggested to be chemopreventive on account of antioxidative properties. Salvia officinalis L., an aromatic and medicinal plant, is widely used in folk medicine and is well known for its antioxidant properties. Therefore, the present study was designed to investigate the antioxidative, possible genotoxic, and antigenotoxic potency of S. officinalis extract against cyclophosphamide (CYP)-induced oxidative stress and genotoxicity in Wistar albino rats. Animals were orally dosed with S. officinalis extract (50, 100, and 150 mg/kg body weight) for 7 days before the administration of a single intraperitoneal dose of CYP (40 mg/kg body weight). The biochemical and cytogenetic determinations were carried out 24 h after CYP injection. The activities of malondialdehyde, superoxide dismutase, and catalase were determined in liver, kidney, and heart tissues. The frequency of micronucleated polychromatic erythrocytes (MNPCEs) and the ratio of polychromatic erythrocytes to normochromatic erythrocytes (PCE/NCE) were also evaluated. Additionally, the antioxidant capacity of the extract was analyzed. The micronucleus assay revealed that all doses of the extract tested presented no genotoxic activity; in addition, the 2 highest doses reduced the MNPCEs and increased the PCE/NCE ratio in the bone marrow and restored the oxidative stress markers in CYP-treated rats. In correlation with these findings, S. officinalis extract exhibited high antioxidant capacity. The results of the present study suggest that the methanolic extract of S. officinalis has a protective effect against CYP-induced oxidative stress and genotoxicity through its antioxidant property.

Protective effects of Salvia officinalis extract against cyclophosphamide-induced genotoxicity and oxidative stress in rats

Medicinal plants, especially rich in polyphenolic compounds, have been suggested to be chemopreventive on account of antioxidative properties. Salvia officinalis L., an aromatic and medicinal plant, is widely used in folk medicine and is well known for its antioxidant properties. Therefore, the present study was designed to investigate the antioxidative, possible genotoxic, and antigenotoxic potency of S. officinalis extract against cyclophosphamide (CYP)-induced oxidative stress and genotoxicity in Wistar albino rats. Animals were orally dosed with S. officinalis extract (50, 100, and 150 mg/kg body weight) for 7 days before the administration of a single intraperitoneal dose of CYP (40 mg/kg body weight). The biochemical and cytogenetic determinations were carried out 24 h after CYP injection. The activities of malondialdehyde, superoxide dismutase, and catalase were determined in liver, kidney, and heart tissues. The frequency of micronucleated polychromatic erythrocytes (MNPCEs) and the ratio of polychromatic erythrocytes to normochromatic erythrocytes (PCE/NCE) were also evaluated. Additionally, the antioxidant capacity of the extract was analyzed. The micronucleus assay revealed that all doses of the extract tested presented no genotoxic activity; in addition, the 2 highest doses reduced the MNPCEs and increased the PCE/NCE ratio in the bone marrow and restored the oxidative stress markers in CYP-treated rats. In correlation with these findings, S. officinalis extract exhibited high antioxidant capacity. The results of the present study suggest that the methanolic extract of S. officinalis has a protective effect against CYP-induced oxidative stress and genotoxicity through its antioxidant property.

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Bibtex @ { tbtkveterinary150653, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {}, publisher = {TÜBİTAK}, year = {2012}, volume = {36}, pages = {646 - 654}, doi = {}, title = {Protective effects of Salvia officinalis extract against cyclophosphamide-induced genotoxicity and oxidative stress in rats}, key = {cite}, author = {Alkan, Fulya Üstün and Gürsel, Feraye Esen and Ateş, Atila and Özyürek, Mustafa and Güçlü, Kubilay and Altun, Mehmet} }
APA Alkan, F , Gürsel, F , Ateş, A , Özyürek, M , Güçlü, K , Altun, M . (2012). Protective effects of Salvia officinalis extract against cyclophosphamide-induced genotoxicity and oxidative stress in rats . Turkish Journal of Veterinary and Animal Sciences , 36 (6) , 646-654 .
MLA Alkan, F , Gürsel, F , Ateş, A , Özyürek, M , Güçlü, K , Altun, M . "Protective effects of Salvia officinalis extract against cyclophosphamide-induced genotoxicity and oxidative stress in rats" . Turkish Journal of Veterinary and Animal Sciences 36 (2012 ): 646-654 <
Chicago Alkan, F , Gürsel, F , Ateş, A , Özyürek, M , Güçlü, K , Altun, M . "Protective effects of Salvia officinalis extract against cyclophosphamide-induced genotoxicity and oxidative stress in rats". Turkish Journal of Veterinary and Animal Sciences 36 (2012 ): 646-654
RIS TY - JOUR T1 - Protective effects of Salvia officinalis extract against cyclophosphamide-induced genotoxicity and oxidative stress in rats AU - Fulya Üstün Alkan , Feraye Esen Gürsel , Atila Ateş , Mustafa Özyürek , Kubilay Güçlü , Mehmet Altun Y1 - 2012 PY - 2012 N1 - DO - T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 646 EP - 654 VL - 36 IS - 6 SN - 1300-0128-1303-6181 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences Protective effects of Salvia officinalis extract against cyclophosphamide-induced genotoxicity and oxidative stress in rats %A Fulya Üstün Alkan , Feraye Esen Gürsel , Atila Ateş , Mustafa Özyürek , Kubilay Güçlü , Mehmet Altun %T Protective effects of Salvia officinalis extract against cyclophosphamide-induced genotoxicity and oxidative stress in rats %D 2012 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 36 %N 6 %R %U
ISNAD Alkan, Fulya Üstün , Gürsel, Feraye Esen , Ateş, Atila , Özyürek, Mustafa , Güçlü, Kubilay , Altun, Mehmet . "Protective effects of Salvia officinalis extract against cyclophosphamide-induced genotoxicity and oxidative stress in rats". Turkish Journal of Veterinary and Animal Sciences 36 / 6 (Haziran 2012): 646-654 .
AMA Alkan F , Gürsel F , Ateş A , Özyürek M , Güçlü K , Altun M . Protective effects of Salvia officinalis extract against cyclophosphamide-induced genotoxicity and oxidative stress in rats. Turkish Journal of Veterinary and Animal Sciences. 2012; 36(6): 646-654.
Vancouver Alkan F , Gürsel F , Ateş A , Özyürek M , Güçlü K , Altun M . Protective effects of Salvia officinalis extract against cyclophosphamide-induced genotoxicity and oxidative stress in rats. Turkish Journal of Veterinary and Animal Sciences. 2012; 36(6): 646-654.
IEEE F. Alkan , F. Gürsel , A. Ateş , M. Özyürek , K. Güçlü ve M. Altun , "Protective effects of Salvia officinalis extract against cyclophosphamide-induced genotoxicity and oxidative stress in rats", Turkish Journal of Veterinary and Animal Sciences, c. 36, sayı. 6, ss. 646-654, Haz. 2012
Turkish Journal of Veterinary and Animal Sciences
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK

61.6b55.9b

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