Prevalence and molecular characteristics of fowladenovirus serotype 4 in eastern Saudi Arabia

Fowl adenovirus serotype 4 (FAdV-4) is a new emerging viral disease of chickens worldwide. It causes inclusion body hepatitis and hepatitis-hydropericardium syndrome. Little is known about its prevalence in the Middle East. Here we report the prevalence of FAdV-4 in five chicken farms in eastern Saudi Arabia. High mortality rates were reported from birds from those five farms at 15 weeks of age. Gross examination revealed typical hydropericardium syndrome and accumulation of jelly-like materials in the pericardial cavities. We isolated FAdV-4 by using embryonated chicken egg inoculation. The inoculated embryos showed dwarfing, deformities, hemorrhage, and death after 3 5 days of inoculations. Detection of FAdV-4 in the heart and liver tissues was achieved by polymerase chain reaction (PCR) and real-time PCR using the primers targeted to the partial hexon gene. Further confirmation was done by restriction fragment length polymorphism and the digestion patterns of the isolated FAdV-4 DNAs were close to those of other known FAdV-4 strains. The average genome size of the virus was ~43 kb. Phylogenetic analysis of the partial hexon gene sequences confirmed that these strains were closely related to other Asian strains from Kuwait, India, and Pakistan reported to GenBank. To our knowledge, this is the first study that reports the isolation and molecular characterization of FAdV-4 in chickens in Saudi Arabia.

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1. Gunes A, Marek A, Hess M. Species determination of fowl adenoviruses based on the 52K gene region. Avian Dis 2013; 57: 290-294.
2. Hess M. Detection and differentiation of avian adenoviruses: a review. Avian Pathol 2000; 29: 195-206.
3. Li H, Wang J, Qiu L, Han Z, Liu S. Fowl adenovirus species C serotype 4 is attributed to the emergence of hepatitis-hydropericardium syndrome in chickens in China. Infect Genet Evol 2016; 45: 230-241.
4. Hess M, Raue R, Prusas C. Epidemiological studies on fowl adenoviruses isolated from cases of infectious hydropericardium. Avian Pathol 1999; 28: 433-439.
5. Asrani RK, Gupta VK, Sharma SK, Singh SP, Katoch RC. Hydropericardium-hepatopathy syndrome in Asian poultry. Vet Rec 1997; 141: 271-273.
6. Balamurugan V, Katari JM, Tiwari AK, Verma KC, Toroghi R, Jadhao SJ. Development of sandwich ELISA for the detection of fowl adenovirus 4 associated with hydropericardium syndrome in experimentally infected chicken. Acta Virol 2001; 45: 95-100.
7. Nakamura K, Mase M, Yamamoto Y, Takizawa K, Kabeya M, Wakuda T, Matsuda M, Chikuba T, Yamamoto Y, Ohyama T et al. Inclusion body hepatitis caused by fowl adenovirus in broiler chickens in Japan, 2009-2010. Avian Dis 2011; 55: 719-723.
8. Meulemans G, Couvreur B, Decaesstecker M, Boschmans M, van den Berg TP. Phylogenetic analysis of fowl adenoviruses. Avian Pathol 2004; 33: 164-170.
9. Meulemans G, Boschmans M, Berg TP, Decaesstecker M. Polymerase chain reaction combined with restriction enzyme analysis for detection and differentiation of fowl adenoviruses. Avian Pathol 2001; 30: 655-660.
10. Abdul-Aziz TA, al-Attar MA. New syndrome in Iraqi chicks. Vet Rec 1991; 129: 272.
11. Nakamura K, Mase M, Yamaguchi S, Yuasa N. Induction of hydropericardium in one-day-old specific-pathogen-free chicks by adenoviruses from inclusion body hepatitis. Avian Dis 2000; 44: 192-196.
12. Parthiban M, Manoharan S, Roy P, Chandran ND, Aruni AW, Koteeswaran A. Nucleotide sequence analysis of the L1 loop variable region of hexon gene of fowl adenovirus 4 isolates from India. Acta Virol 2005; 49: 65-68.
13. Steer PA, Kirkpatrick NC, ORourke D, Noormohammadi AH. Classification of fowl adenovirus serotypes by use of high-resolution melting-curve analysis of the hexon gene region. J Clin Microbiol 2009; 47: 311-321.
14. Mittal D, Jindal N, Tiwari AK, Khokhar RS. Characterization of fowl adenoviruses associated with hydropericardium syndrome and inclusion body hepatitis in broiler chickens. VirusDisease 2014; 25: 114-119.
15. Lone NA, Rehmani SF, Kazmi SU, Muzaffar R, Khan TA, Khan A, Khan SA, Ahmed A. Molecular characterization of Pakistani field isolates of infectious bursal disease virus. Avian Dis 2009; 53: 306-309.
16. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 2013; 30: 2725-2729.
17. Griffin BD, Nagy E. Coding potential and transcript analysis of fowl adenovirus 4: insight into upstream ORFs as common sequence features in adenoviral transcripts. J Gen Virol 2011; 92: 1260-1272.
18. Wilson FD, Wills RW, Senties-Cue CG, Maslin WR, Stayer PA, Magee DL. High incidence of glomerulonephritis associated with inclusion body hepatitis in broiler chickens: routine histopathology and histomorphometric studies. Avian Dis 2010; 54: 975-980.
19. Jadhao SJ, Deepak JN, Kataria JM, Kataria RS, Tiwari AK, Somvanshi R, Sangamithra P, Verma KC. Characterisation of fowl adenoviruses from chickens affected with infectious hydropericardium during 1994-1998 in India. Indian J Exp Biol 2003; 41: 321-327.
20. Mansoor MK, Hussain I, Arshad M, Muhammad G. Preparation and evaluation of chicken embryo-adapted fowl adenovirus serotype 4 vaccine in broiler chickens. Trop Anim Health Prod 2011; 43: 331-338.
21. Rahul S, Kataria JM, Senthilkumar N, Dhama K, Sylvester SA, Uma R. Association of fowl adenovirus serotype 12 with hydropericardium syndrome of poultry in India. Acta Virol 2005; 49: 139-143.
22. Okuda Y, Ono M, Shibata I, Sato S, Akashi H. Comparison of the polymerase chain reaction-restriction fragment length polymorphism pattern of the fiber gene and pathogenicity of serotype-1 fowl adenovirus isolates from gizzard erosions and from feces of clinically healthy chickens in Japan. J Vet Diagn Invest 2006; 18: 162-167.
23. Ojkic D, Krell PJ, Tuboly T, Nagy E. Characterization of fowl adenoviruses isolated in Ontario and Quebec, Canada. Can J Vet Res 2008; 72: 236-241.
24. Li L, Luo L, Luo Q, Zhang T, Zhao K, Wang H, Zhang R, Lu Q, Pan Z, Shao H et al. Genome sequence of a fowl adenovirus serotype 4 strain lethal to chickens, isolated from China. Genome Announc 2016; 4: e00140-16.