Pregnancy diagnosis in mares by determination of oestradiol-17- hormone in faeces

Bu çalışmada kısrak dışkısında östradiol-17 ß hormonu EIA tekniği ile ölçülerek gebeliğin teşhis edilmesi amaçlandı. Çalışma 30 kısrak üzerinde gerçekleştirildi. Gebelik boyunca bir ay aralıklarla dışkı örnekleri toplandı. Gebeliğin 4. ayında ortalama Östradiol 17-ß konsantrasyonları gebelerde 9.39 ± 2.63, gebe olmayanlarda 7.70 ± 2.00 ng/g ölçüldü. Gebeliğin 5. ayından itibaren östradiol-17 ß miktarı gebe kısraklarda gebe olmayanlardan belirgin derecede yüksekti, 5. ayda gebelerde 71.20 ± 8.00 gebe olmayanlarda ise 4.60 ± 2.20 ng/g bulundu. E1A metodu ile kısraklarda 5. aydan itibaren gebeliğin teşhis edilmesi mümkün olmasına karşın, gebelerdeki östradiol-17 ß miktarının gebe olmayanlara göre ne zaman farklılaşmaya başladığının belirlenmesi için dışkı örneklerinin daha kısa aralıklarla toplanmasının uygun olacağı kanısına varıldı.

Kısrak dışkısında östradiol 17- hormonu tayini ve gebeliğin teşhisi

The aim of the present study was the confirmation of pregnancy diagnosis in mares by the determination of oestradiol-17 ß in faeces. The research was carried out on 30 cyclic mares. Faeces samples were collected through pregnancy at monthly intervals. The faeces samples of pregnant and non-pregnant mares contained a mean oestradiol-17 ß concentration of 9.39 ± 2.63 and 7.70 ± 2.00 ng/g, respectively in the 4th month of gestation. From the 5th month of pregnancy the oestradiol-17 ß concentration in pregnant mares was significantly higher than in non-pregnant mares. Oestradiol-17 ß levels were estimated to be 71.20 ± 8.00 and 4.60 ± 2.20 ng/g in pregnant and non-pregnant mares in the 5th month of pregnancy, respectively. However, the confirmation of pregnancy with the enzyme - immunoassay technique was possible after the 5th month of gestation; faeces samples collected at short intervals would be necessary to obtain a reliable pregnancy diagnosis.

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