Plasma Lysozyme Levels and Secondary Stress Response in Rainbow Trout, Oncorhynchus mykiss (Walbaum) after Exposure to Leteux-Meyer Mixture

Leteux-Meyer mixture (LMM) contains formalin and malachite green and can be externally applied to fish as an antimicrobial agent. To determine the effects of prophylactic treatment with LMM on plasma lysozyme levels and the secondary stress indices, alterations in plasma glucose, chloride, sodium, potassium and hematocrit were monitored in healthy rainbow trout, Oncorhynchus mykiss W. Fish were i) exposed to 25 ppm Leteux-Meyer mixture for 60 min and ii) the same treatment followed by 60 min recovery in clean freshwater. Treatment with LMM resulted in a decrease in plasma lysozyme values. After a recovery period of 60 min plasma lysozyme levels were not restored to control values. Treatment of fish with LMM elicited marked elevations in plasma glucose and plasma potassium values. Plasma chloride and sodium values dropped after LMM treatment. Recovery for 60 min was not sufficient to restore the electrolyte levels. Hematocrit values were not affected by LMM treatment.

Plasma Lysozyme Levels and Secondary Stress Response in Rainbow Trout, Oncorhynchus mykiss (Walbaum) after Exposure to Leteux-Meyer Mixture

Leteux-Meyer mixture (LMM) contains formalin and malachite green and can be externally applied to fish as an antimicrobial agent. To determine the effects of prophylactic treatment with LMM on plasma lysozyme levels and the secondary stress indices, alterations in plasma glucose, chloride, sodium, potassium and hematocrit were monitored in healthy rainbow trout, Oncorhynchus mykiss W. Fish were i) exposed to 25 ppm Leteux-Meyer mixture for 60 min and ii) the same treatment followed by 60 min recovery in clean freshwater. Treatment with LMM resulted in a decrease in plasma lysozyme values. After a recovery period of 60 min plasma lysozyme levels were not restored to control values. Treatment of fish with LMM elicited marked elevations in plasma glucose and plasma potassium values. Plasma chloride and sodium values dropped after LMM treatment. Recovery for 60 min was not sufficient to restore the electrolyte levels. Hematocrit values were not affected by LMM treatment.

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  • Thorburn, M.A., Moccia, R.D.: Use of chemotherapeutics on trout farms in Ontario. J. Aquat. Anim. Health, 1993; 5: 85-91.
  • Papoutsoglou, S.E., Miliou, H., Chadio, S., Karakatsouli, N., Zarkada, A.: Studies on stress responses and recovery from removal in gilthead sea bream Sparus aurata (L.) using recirculated seawater system. Aquacult. Eng., 1999; 21: 19-32.
  • Pironet, F.N., Jones, J.B.: Treatments for ectoparasites and diseases in captive Western Australian dhufish. Aquacult. Int., 2000; 8: 349-361.
  • Bills, T.D., Hunn, J.B.: Changes in the blood chemistry of Coho salmon exposed to malachite green. Prog. Fish Cult., 1976; 38: 214-216. 5. Grizzle, J.M.: Hemalotogical changes in fingerling channel catfish exposed to malachite green. Prog. Fish Cult., 1977; 39: 90-93.
  • Hlavek, R.R., Bulkley, R.V.: Effects of malachite green on leucocyte abundance in rainbow trout, Salmo gairdneri (Richardson). J. Fish Biol., 1980; 17: 431-444.
  • Kakuta, I., Namba, K., Uematsu, K., Murachi, S.: Physiological response of the fish, Cyprinus carpio, to formalin exposure. I. Effects of formalin on urine flow, heart rate, respiration. Comp. Biochem. Physiol. C, 1991; 100: 405-411.
  • Yıldız, H.Y., Pulatsü, S.: Evaluation of the secondary stress response in healthy Nile tilapia (Oreochromis niloticus L.) after treatment with a mixture of formalin, malachite green and methylene blue. Aquac. Res., 1999; 30: 379-383.
  • Srivastava, S.J., Singh, N.D., Srivastava, A.K., Sinha, R.: Acute toxicity of malachite green and its effects on certain blood parameters of a catfish, Heteropneustes fossilis. Aquat. Toxicol., 1995; 31: 241-247.
  • Srivastava, S.J., Sinha, R., Roy, D.: Toxicological effects of malachite green. Aquat. Toxicol., 2004; 66: 319-329.
  • Leteux, F., Meyer, F.P.: Mixtures of malachite green and formalin for controlling Ichthyophthirius multifiliis and other protozoan parasites of fish. Prog. Fish Cult., 1972; 34: 21-26.
  • Treves-Brown, K. M.: Applied Fish Pharmacology. 2000. Kluwer Academic Publishers, Netherlands.
  • Demers, N.E., Bayne, C.J.: The immediate effects of stress on hormones and plasma lysozyme in rainbow trout. Dev. Comp. Immunol., 1997; 21: 363-373.
  • Watts, M., Munday, B.L., Burke, C.M.: Immune responses of teleost fish. Aust. Vet. J., 2001; 79: 570-574.
  • Ellis, A.E.: Immunity to bacteria in fish. Fish Shell. Immunol., 1999; 9: 291-308.
  • Caruso, D., Schlumberger, O., Dahm, C., Proteau, J.P.: Plasma lysozyme levels in sheatfish Silurus glanis (L.) subjected to stress and experimental infection with Edwarsiella tarda. Aquac. Res., 2002; 33: 999-1008.
  • Ellis, A.E.: Lysozyme assays. In Technigues in Fish Immunology (ed. By J.S. Stolen, T.C. Fletcher, D.P. Anderson, B.S. Robertson & W.B. van Muisvinkel) 1990; 101-103. SOS Publications, Fair Haven, NJ, USA.
  • Siwicki, A.K., Anderson, D.P.: Immunostimulation in fish. In: Proceedings of the Nordic Symposium on Fish Immunology, 1993. Sweden, 19-22 May.
  • Ruane, N.M., Nolan, D.T., Rotllant, J., Costelloe, J., Wendelaar Bonga, S.E.: Experimental exposure of rainbow trout Oncorhynchus mykiss (Walbaum) to the infective stages of the sea louse Lepeophtheirus salmonis physiological response to an acute stressor. Fish and Shell. Immunol., 2000; 10: 451-463.
  • (Kr´İyer) influences the
  • Fevolden, S.E., Refstie, T., Roed, K.H.: Disease resistance in rainbow trout, Onchorynchus mykiss selected for stress response. Aquaculture, 1992; 104: 19-29.
  • Fivelstad, S., Schwarz, J., Stromsnes, H.: Sublethal effects and safe levels of ammonia in seawater for Atlantic salmon postsmolts (Salmo salar L.) Aquacult. Eng., 1995; 14: 271-280.
  • Barham, W.T., Smith, G.L., Schoonbee, H.J.: The haematological assessment of bacterial infection in rainbow trout, Salmo gairdneri Richardson. J. Fish Biol., 1980; 17: 275-281.
  • Pickering, A.D.: Rainbow trout husbandary: management of the stress response. Aquaculture, 1992; 100: 125-139.
  • Postlethwaite, E.K., McDonald, D.G.: Mechanisms of Na+and Cl- regulation in freshwater adapted rainbow trout (Oncorhynchus mykiss) during exercise and stress. J. Exp. Biol., 1995; 198: 295- 304.
  • Sanchez, J.G., Speare, D.J., Johnson, G.J., Horney, B.S.: Evaluation of the stress response in healthy juvenile rainbow trout after repetitive intermittent treatment with chloramine-t or formalin. J. Aquat. Anim. Health, 1997, 9: 301-308.
  • Yamamoto, K.I.: Effects of formalin on gas exchange in the gills of carp, Cyprinus carpio. Comp. Biochem. Physiol. C, 1991; 98: 463-465.
  • Vosyliene, M.Z., Kazlauskiene, N.: Alterations in fish health state parameters after exposure to different stressors. Acta Zool. Lit. Hydrobiol., 1999; 9: 83-94.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
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