Phenotypic characterization and differentiation of mesenchymal stem cells originating from adipose tissue

Öz Stem cells are known as undifferentiated cells that are capable of divisions and self-renewal over an extended period. Adipose tissue-derived mesenchymal stem cells (ADSCs) are known to differentiate into many different organ and tissue cells. The purpose of this study was to characterize adipose tissue-derived mesenchymal stem cells in detail based on the expression status of certain surface markers and differentiation stages for clinical and laboratory applications. The migrating phase and mitotic property of the cells from adipose tissue were assessed under a microscope. Cell counting was performed during the first, second, and third passages. The cells were differentiated into osteogenic, chondrogenic, and adipogenic lineages. Stem cells were characterized by immunostaining of CD90, CD105, CD11b, and CD45 surface markers. ADSC growth kinetics were determined at the first, second, and third passages. In conclusion, ADSCs were reported in detail here based on surface markers and differentiation potentials, growth curves, and population doubling times during the first, second, and third passages.

Kaynak Göster

Bibtex @ { tbtkveterinary663797, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {}, publisher = {TÜBİTAK}, year = {2019}, volume = {43}, pages = {834 - 845}, doi = {}, title = {Phenotypic characterization and differentiation of mesenchymal stem cells originating from adipose tissue}, key = {cite}, author = {Çerçi, Ece and Erdost, Hatice} }
APA Çerçi, E , Erdost, H . (2019). Phenotypic characterization and differentiation of mesenchymal stem cells originating from adipose tissue . Turkish Journal of Veterinary and Animal Sciences , 43 (6) , 834-845 . Retrieved from https://dergipark.org.tr/tr/pub/tbtkveterinary/issue/50881/663797
MLA Çerçi, E , Erdost, H . "Phenotypic characterization and differentiation of mesenchymal stem cells originating from adipose tissue" . Turkish Journal of Veterinary and Animal Sciences 43 (2019 ): 834-845 <https://dergipark.org.tr/tr/pub/tbtkveterinary/issue/50881/663797>
Chicago Çerçi, E , Erdost, H . "Phenotypic characterization and differentiation of mesenchymal stem cells originating from adipose tissue". Turkish Journal of Veterinary and Animal Sciences 43 (2019 ): 834-845
RIS TY - JOUR T1 - Phenotypic characterization and differentiation of mesenchymal stem cells originating from adipose tissue AU - Ece Çerçi , Hatice Erdost Y1 - 2019 PY - 2019 N1 - DO - T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 834 EP - 845 VL - 43 IS - 6 SN - 1300-0128-1303-6181 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences Phenotypic characterization and differentiation of mesenchymal stem cells originating from adipose tissue %A Ece Çerçi , Hatice Erdost %T Phenotypic characterization and differentiation of mesenchymal stem cells originating from adipose tissue %D 2019 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 43 %N 6 %R %U
ISNAD Çerçi, Ece , Erdost, Hatice . "Phenotypic characterization and differentiation of mesenchymal stem cells originating from adipose tissue". Turkish Journal of Veterinary and Animal Sciences 43 / 6 (Aralık 2019): 834-845 .
AMA Çerçi E , Erdost H . Phenotypic characterization and differentiation of mesenchymal stem cells originating from adipose tissue. Turkish Journal of Veterinary and Animal Sciences. 2019; 43(6): 834-845.
Vancouver Çerçi E , Erdost H . Phenotypic characterization and differentiation of mesenchymal stem cells originating from adipose tissue. Turkish Journal of Veterinary and Animal Sciences. 2019; 43(6): 834-845.
IEEE E. Çerçi ve H. Erdost , "Phenotypic characterization and differentiation of mesenchymal stem cells originating from adipose tissue", Turkish Journal of Veterinary and Animal Sciences, c. 43, sayı. 6, ss. 834-845, Ara. 2019

5942 5870

Arşiv
Sayıdaki Diğer Makaleler

On-farm diagnosis of latent respiratory failure in calves

Anton CHERNITSKIY, Sergey SHABUNIN, Tatiana KUCHMENKO, Vladimir SAFONOV

Animal remains of Alaybeyi Höyük

Abu Bakar SIDDIQ

A diagnostic survey of Neospora caninum infection in aborted fetuses in the Middle Black Sea Region and Sivas Province, Turkey

Mustafa AÇICI, Cenk Soner BÖLÜKBAŞ, Gökmen Zafer PEKMEZCİ, Hande GÜRLER, Oktay GENÇ, Ali Tümay GÜRLER, Selma KAYA, Şinasi UMUR

Neutralizing antibody titers against field strains of bovine viral diarrhea virus after vaccination with three commercial vaccines

Gizem ALPAY, Kadir YEŞİLBAĞ

Effect of exercise on some minerals, metabolites and enzyme activities in the serum of trained Arabian horses

Roya POURMOHAMMAD, Mehrdad MOHRI, Hesam SEIFI, Kamran SARDARI

Study of retail egg supply chain for quality in relation to level of sanitization and farm of origin

Vinayananda Chilur OMKARAPPA, Mohamed Nadeem FAIROZE, Madhavaprasad Chakkodabail BENAKABHAT, Kaustubh BHAVE, Nagappa KARABASANAVAR

Antimicrobial resistance and phenotypic and molecular detection of extended-spectrum ß-lactamases among extraintestinal Escherichia coli isolated from pneumonic and septicemic sheep and goats in Rajasthan, India

Fateh SINGH, Ganesh Gangaram SONAWANE, Jyoti KUMAR, Shivendra Kumar DIXIT, Rajendra Kumar MEENA, Bhupendra Nath TRIPATHI

Development of spermatogonial stem cell niche and immunoexpression of vimentin filaments in the testes of prenatal and postnatal Indian buffalo (Bubalus bubalis)

Ashritha Quanisha CARLO, Devendra PATHAK, Opinder SINGH, Neelam BANSAL

Genotyping of prolactin, kappa casein, and pituitary transcription factor 1 genes of the Anatolian water buffalo population in the Kızılırmak Delta

Emine TOPARSLAN, Levent MERCAN

Histomorphological and histochemical structure of the midgut and hindgut of the Caucasian honey bee (Apis mellifera caucasia)

Ahmet CEYLAN, Sedat SEVİN, Özge ÖZGENÇ