Pathological and clinical findings and tissue distribution of Salmonella Gallinarum infection in turkey poults
This study aimed to experimentally induce Salmonella enterica subsp. enterica serovar Enteritidis (Salmonella Gallinarum) infection in turkey poults in order to detect S. Gallinarum using immunohistochemical, bacteriological, and histological procedures. The study included 90 white turkey poults that were divided into 3 groups (1-day-old, 3-week-old, and 2-month-old), each of which was orally inoculated with an inoculum of 0.3 mL of S. Gallinarum strain 9 broth culture that contained approximately 2 × 104, 1 × 106, and 1 × 109 cfu/mL, respectively. The duration of the experiment was 18 days. Clinical, pathological, and bacteriological findings were evaluated daily until 18 days post inoculation (pi). The most evident clinical symptoms in all 3 groups were diarrhea and somnolence, especially in 1-day-old group. S. Gallinarum strain 9 was isolated from organs and cloacal swabs up to 16 days pi. Inflammation and typical granulomatous nodules in the internal organs were observed in all 3 groups. Strong immunoreactivity was determined in the lungs, bursa of Fabricius, caecum, ileum, and cloaca of all infected poults up to 18 days pi. In conclusion, S. Gallinarum infection caused heterophilic granulomas, especially in the duodenum of turkey poults, and immunohistochemical analysis can be considered as an adjunct to bacteriological methods in the diagnosis of S. Gallinarum infection.
Pathological and clinical findings and tissue distribution of Salmonella Gallinarum infection in turkey poults
This study aimed to experimentally induce Salmonella enterica subsp. enterica serovar Enteritidis (Salmonella Gallinarum) infection in turkey poults in order to detect S. Gallinarum using immunohistochemical, bacteriological, and histological procedures. The study included 90 white turkey poults that were divided into 3 groups (1-day-old, 3-week-old, and 2-month-old), each of which was orally inoculated with an inoculum of 0.3 mL of S. Gallinarum strain 9 broth culture that contained approximately 2 × 104, 1 × 106, and 1 × 109 cfu/mL, respectively. The duration of the experiment was 18 days. Clinical, pathological, and bacteriological findings were evaluated daily until 18 days post inoculation (pi). The most evident clinical symptoms in all 3 groups were diarrhea and somnolence, especially in 1-day-old group. S. Gallinarum strain 9 was isolated from organs and cloacal swabs up to 16 days pi. Inflammation and typical granulomatous nodules in the internal organs were observed in all 3 groups. Strong immunoreactivity was determined in the lungs, bursa of Fabricius, caecum, ileum, and cloaca of all infected poults up to 18 days pi. In conclusion, S. Gallinarum infection caused heterophilic granulomas, especially in the duodenum of turkey poults, and immunohistochemical analysis can be considered as an adjunct to bacteriological methods in the diagnosis of S. Gallinarum infection.
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- Shivaprasad, H.L.: Pullorum disease and fowl typhoid. In: Saif, Y.M., Barnes, H.]., Glisson, ].R., Fadly, A.M., McDougald, L.R., Swayne, D.E., Eds., Diseases of Poultry, 11“I edn. Iowa, USA: Iowa State Press. 2003; 568—582.
- Shivaprasad, H.L.: Fowl typhoid and pullorum disease. Rev. Sci. Tech. Off. Int. Epiz., 2000; 19: 405-424.
- Barrow, P.A.: Salmonella control—past, present and future. Avian Pathol., 1993; 22: 651—669.
- Williams Smith, H., Tucker, I.F.: The virulence of Salmonella strains for chickens: their excretion by infected chickens. I. Hyg. (Lond), 1980; 84: 479—488.
- Humbert, F., Salvat, G.: The risk of transmission of Salmonellae in poultry farming: detection and prevention in Europe. Rev. Sci. Tech. Off. Int. Epiz., 1997; 16: 83—90. (article in French with an abstract in English)
- Mead, G.C.: Prospects for ‘competitive exclusion’ treatment to control Salmonellas and other food borne pathogens in poultry. Vet. I., 2000; 159: 111—123.
- Arora, A.K., Gupta S.C., Kaushik, R.K.: Isolation of Salmonella gallinarum from turkeys. Ind. Vet. I., 1988; 65: 171.
- Beyaz, L., Kutsal, 0.: Pathological and immunohistochemical studies in experimental Salmonella gallinarum infection (fowl typhoid) in chickens. Ankara Üniv. Vet. Fak. Derg., 2003; 50: 219—227. (article in Turkish with an abstract in English)
- McRill, C.M., Kramer, T.T., Griffith, R.W.: Application of the peroxidase-antiperoxidase immunoassay to the identiŞcation of Salmonellae from pure culture and animal tissue. I. Clin. Microbiol, 1984; 20: 281—284. The results of the present study have shown that white turkey poults were slightly susceptible to infection caused by S. Gallinarum. S. Gallinarum infection caused heterophilic granulomas, especially in the duodenum of turkey poults, as well as cystic, degenerative, and necrotic changes in the bursa of
- Fabricius. Immunohistochemical analysis can be
- considered a useful tool for detecting S. Gallinarum
- at various phases of infection and it can be used as an
- adjunct to bacteriological examination in the
- diagnosis of S. Gallinarum.