Oxidative stress in Shaal sheep of different age groups
Oxidative stress plays an important role in the etiology and/or progression of a number of diseases and aging. In this cross-sectional study, some oxidative stress biomarkers (malondialdehyde [MDA] as a lipid peroxidation biomarker, ferric reducing/antioxidant power [FRAP], and total nonprotein SH groups) in plasma samples of Shaal ewes of different age groups were assessed. For all 3 measured parameters, the youngest age group (10-30 months old) had the lowest amounts. Although FRAP levels increased slightly and age dependently, nonprotein SH group content showed a decreasing trend after a peak in the 31-50 months group. The highest MDA concentration was observed in the oldest group. Our results suggest a relation between age and oxidative stress parameters in the early life of Shaal ewes. It also showed an age dependent increase in FRAP levels.
Oxidative stress in Shaal sheep of different age groups
Oxidative stress plays an important role in the etiology and/or progression of a number of diseases and aging. In this cross-sectional study, some oxidative stress biomarkers (malondialdehyde [MDA] as a lipid peroxidation biomarker, ferric reducing/antioxidant power [FRAP], and total nonprotein SH groups) in plasma samples of Shaal ewes of different age groups were assessed. For all 3 measured parameters, the youngest age group (10-30 months old) had the lowest amounts. Although FRAP levels increased slightly and age dependently, nonprotein SH group content showed a decreasing trend after a peak in the 31-50 months group. The highest MDA concentration was observed in the oldest group. Our results suggest a relation between age and oxidative stress parameters in the early life of Shaal ewes. It also showed an age dependent increase in FRAP levels.
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- Dalle-Donne, I., Rossi, R., Giustarini, D., Milzani, A., Colombo,
- R.: Protein carbonyl groups as biomarkers of oxidative stress.
- Clin. Chim. Acta, 2003; 329: 23-38.
- Habif, S., Mutaf, I., Turgan, N., Onur, E., Duman, C., Özmen, D., Bayindir, O.: Age and gender dependent alterations in the activities of glutathione related enzymes in healthy subjects. Clin. Biochem., 2001; 34: 667-671.
- Berger, M.M.: Can oxidative damage be treated nutritionally? Clin. Nutr., 2005; 24: 172-183.
- Cadet, J., Delatour Douki, T., Gasparutto, D., Pouget, J., Ravanat, J., Sauvaigo, S.: Hydroxyl radicals and DNA damage. Mutat. Res., 1999; 424: 9-21.
- Halliwell, B., Gutteridge, J.M.C.: Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem. J., 1984; 219: 1- 14.
- Harman, D.: Aging: a theory based on free radical and radiation chemistry. J. Gerontol., 1956; 11: 298-300.
- Harman, D: The aging process: major risk factor for disease and death. Proc. Nat. Acad. Sci. USA, 1991; 88: 5360-5363.
- Ashok, B.T., Ali., R.: The aging paradox: free radical theory of aging. Exp. Gerontol., 1999; 34: 293-303.
- Cherubini, A., Ruggiero, C., Polidori, M.C., Mecocci, P.: Potential markers of oxidative stress in stroke. Free Radic. Biol. Med., 2005; 39: 841-852.
- Satho, K.: Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin. Chim. Acta, 1978; 90: 37-43.
- Benzi, I.F., Strain, J.J.: Ferric reducing/antioxidant assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Method. Enzymol., 1999; 292: 15-27.
- Hu, M.L., Dillar, C.J.: Assessing molecular, cell and tissue damage, plasma SH and CSH: measurement of protein thiol groups and glutathione in plasma. Method. Enzymol., 1994; 233: 385-387.
- Nussey, D.H., Pemberton, J.M., Pilkington, J.G., Blount, J.D.: Life history correlates of oxidative damage in a free-living mammal population. Funct. Ecol., 2009; 23: 809-817.
- Das, S., Vasisht, S., Snehlata, Das, N., Srivastava, L.M.: Correlation between total antioxidant status and lipid peroxidation in hypercholesterolemia. Curr. Sci. India, 2002; 78: 486-487.
- Pansarasa, O., Bertorelli, L., Vecchiet, J., Felzani, G., Marzatico, F.: Age-dependent changes of antioxidant activities and markers of free radical damage in human skeletal muscle. Free Radical Bio. Med., 1999; 27: 617-622.
- Junqueira, V.B.C., Barros, S.B.M., Chan, S.S., Rodrigues, L., Giavarotti, L., Abud, R.L., Deucher, G.P.: Aging and oxidative stress. Mol. Aspects Med., 2004; 25: 5-16.
- Mézes, M., Sályi, G.: Effect of acute selenium toxicosis on the lipid peroxide status and the glutathione system of broiler chickens. Acta Vet. Hung., 1994; 42: 459-463.
- Valls, V., Peiro, C., Muñiz, P., Saez, G.T.: Age-related changes in antioxidant status and oxidative damage to lipids and DNA in mitochondria of rat liver. Process Biochem., 2005; 40: 903-908.
- Muradian, K.K., Utko, N.A., Fraifeld, V., Mozzhukhina, T.G., Pishel, I.N., Litoshenko, A.Y.: Superoxide dismutase, catalase and glutathione peroxidase activities in the liver of young and old mice: linear regression and correlation. Arch. Gerontol. Geriatr., 2002; 35: 205-214.
- Argüelles, S., García, S., Maldonado, M., Machado, A., Ayala, A.: Do the serum oxidative stress biomarkers provide a reasonable index of the general stress status? Biochim. Biophys. Acta, 2004; 1674: 251-259.
- Yagi, K.: Lipid peroxides, free radicals, and diseases. In: Active Oxygens, Lipid Peroxides and Antioxidants. Japan Science Press, Tokyo - CRC Press, Boca Raton. 1993; 39-50.
- Stadtman, E.R.: Importance of individuality in oxidative stress and aging. Free Radical Biol. Med., 2002; 33: 597-604.