Lyudmila UZENBAEVA, Viktor ILYUKHA, Nikolay TYUTYUNNIK, Aleksandra KIZHINA

Morphological abnormalities of blood and bone marrow leukocytes and age-related changes in different leukocyte counts in the American mink (Neovison vison)

The sapphire mink is a unique animal model to study cellular morphology. It demonstrates abnormal leukocytes like in patients with Chédiak Higashi syndrome. We aimed to determine the morphologic features of blood and bone marrow leukocytes in the sapphire mink and dark-brown mink (wild type). In addition, were assessed age-related changes in total white blood cell (WBC) and differential WBC counts. We conclude that the sapphire mink has a morphological leukocyte defect (abnormal granules). We observed that abnormal granules formed at an early stage of bone marrow leukocyte maturation. It was shown that the leukocyte profile of young mink differs from that of adult animals. The levels of abnormal segmented neutrophils and abnormal lymphocytes were higher in adult mink as compared with puppies (4-, 10-day-old).

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1. Trapezov OV, Trapezova LI, Alekhina TA, Klochkov DV, Ivanov IN. Effects of monorecessive and double recessive mutations affecting coat color on the monoamine content of the brain of the American mink (Neovison vison Schreber, 1777). Russian Journal of Genetika 2009; 45: 1641-1645.
2. Vagin IV. Effect of aleutian gene on mink (Neovison vison) growth. Cytology and Genetika 2003; 37: 16-19 (article in Russian with an English abstract).
3. Oliver C, Essner E. Formation of anomalous lysosomes in monocytes, neutrophils, and eosinophils from bone marrow of mice with Chediak-Higashi syndrome. J Histochem Cytochem 1973; 32: 17-27.
4. Uzenbaeva LB, Trapezov OV, Kizhina AG, Iliukha VA, Trapezova LI, Tiutiunnik NN. Effect of mutations affecting coat color on the blood lymphocyte structure in the American mink (Neovison vison Schreber, 1777). Genetika 2011; 47: 87-94.
5. Kramer JW, Davis WC, Prieur DJ. The Chediak-Higashi syndrome of cats. Lab Invest 1977; 36: 554-562.
6. Nishimura M, Inoue M, Nakano T, Nishikawa T, Miyamoto M, Kobayashi T, Kitamura Y. Beige rat a new animal model of Chediak-Higashi syndrome. Blood 1989; 74: 270-273.
7. Oliver C, Essner E. Distribution of anomalous lysosomes in the beige mouse: a homologue of Chediak-Higashi syndrome. J Histochem Cytochem 1973; 21: 218-228.
8. Ozaki K, Maeda H, Uechi S, Yamate H, Nishikawa T, Nishimura M, Narama I. The presence of giant granules in the juxtaglomerular cells of beige rats may affect plasma rennin activity and blood pressure. Exp Mol Path 1994; 61: 221-229.
9. Anistoroaei R, Krogh AK, Christensen K. A frameshift mutation in the LYST gene is responsible for the Aleutian color and the associated ChédiakHigashi syndrome in American mink. Anim Genet 2012; 44: 178-183.
10. Blume RS, Padgett GA, Wolf SM, Bennett JM. Neutrophil granules in the Chediak-Higashi syndrome of man, mink, cattle and mice. Can J Comp Med 1969; 33: 271-274.
11. Hammer AS, Andersen TH, Eriksen T, Kortegaard HE, Dietz HH, Chriél M. Radiographic evaluation of destructive periodontal disease in blue mink in relation to age and blood morphology. Can J Vet Res 2005; 69: 128-134.
12. Aulerich RJ, Heil CR, Lehning EL, Ringer RK. Leukocyte ratios kits from birth to 10 age. Scientifur 1976; 13: 104-110.
13. Coe JE, Race RE. Ontogeny of mink IgG, IgA, and IgM. Proc Soc Exp Biol Med 1978; 157: 289-292.
14. Jain NC. Comparative hematologic features of some avian and mammalian species. In: Jain NC, editor. Essentials of Veterinary Hematology. 1st ed. Philadelphia, PA, USA: Lea and Febiger; 1993. pp. 54-71.
15. Kojima S, Haruta J, Enomota A, Fujisawa H, Harada T, Maita K. Age-related hematological changes in normal F344 rats: during the neonatal period. Exp Anim 1999; 48: 153-159.
16. Zelnickova P, Kovaru H, Pesak S, Lojek A, Matalova E, Ondracek J, Kovaru F. Postnatal function maturation of phagocytes in pig. Vet Immunol Immunopaphol 2008; 113: 383-391.
17. Fidler IJ. Lymphocytes are not only immunocytes. Biomed 1980; 32: 1-3.
18. White JR, Gong H, Moreland JG, Flaherty H, McElroy SJ. Evaluation of hematologic in newborn C57/BL6 mice. Vet Clin Paphol 2016; 45: 87-95.
19. Filias A, Theodorou L, Mouzopoulou S, Varvarigou A, Mantagous S, Karakantza M. Phagocytic ability of neutrophils and monocytes in neonates. BMC Pediatr 2011; 11: 1471-1476.
20. Jørgensen G. Mink Production. Hilleroed, Denmark: Scientifur, 1985.21. Fagerland JA, Hagemoser WA, Ireland WP. Ultrastructure and stereology of leukocytes and platelets of normal foxes and a fox with Chediak-Higashi-like syndrome. Vet Paph 1987; 24: 164-169.
22. Spicer SS, Sato A, Vincent R, Eguchhi M, Poon KC. Lysosome enlargement in the Chediak-Higashi syndrome. Fed Proc 1981; 40: 1451-1455.
23. Harvey JW.Canine bone marrow: normal hematopoiesis, biopsy techniques, and cell identification and evaluation. Compend Cont Educ Pract Vet1984; 6: 909-926.
24. Delcourt-Debruyne EM, Boutigny HR, Hildebrand HF. Features of severe periodontal disease in a teenager with Chédiak-Higashi syndrome. J Periodontol 2000; 71: 816-824.
25. Davis WC, Spicer SS, Greene WB, Padgett GA. Ultrastructure of cell in bone marrow of normal mink and mink with the homologue of the Chediak-Higashi trait of humans. II. Cytoplasmic granules in eosinophils, basophils, mononuclear cells and platelets. Am J Path 1971; 63: 411-427.