Nikolaos SOULTOS, Eleni IOSSIFIDOU, Amin AMBRAHIM, Evdoxios PSOMAS, Ioannis TZAVARAS, Demetrios KOUTSOPOULOS, Thomai LAZOU

Listeria monocytogenes in mussels (Mytilus galloprovincialis) harvested from North Aegean coastal area

One hundred and two samples of mussels (Mytilus galloprovincialis), harvested from approved shellfish coastal water in northern Greece, were screened for the presence and antimicrobial resistance of Listeria monocytogenes. Listeria spp. were isolated according to International Organization for Standardization method 11290-1: 1996/FDAM 1: 2004(E) and identified using a multiplex polymerase chain reaction (PCR) system. The serovar identity of L. monocytogenes isolates was also determined with a multiplex PCR assay. The antimicrobial profile of the isolates was determined by the disk diffusion method. Listeria spp. were present in 8 of 102 samples tested (8%) and only 1 (1%) yielded L. monocytogenes. The isolate identified as L. monocytogenes was defined as serogroup I and found to be resistant to nalidixic acid and streptomycin. In conclusion, this study demonstrated that L. monocytogenes is not commonly found in mussels harvested in the North Aegean Sea, whereas there is a higher possibility of mussels’ contamination with other Listeria species.

Anahtar Kelimeler:

Key words: Listeria monocytogenes, mussels, Mytilus galloprovincialis, antibiotic resistance, serotyping

Listeria monocytogenes in mussels (Mytilus galloprovincialis) harvested from North Aegean coastal area

Keywords:

Key words: Listeria monocytogenes, mussels, Mytilus galloprovincialis, antibiotic resistance, serotyping,

PDF

___

General Secretariat of National Statistical Service of Greece. Quantity of Seafoods. Table B4. Athens, Greece: General Secretariat of National Statistical Service of Greece; 2009.
Swaminathan B, Cabanes D, Zhang W, Cossart P. L. monocytogenes. In: Doyle MP, Beuchat LR, editors. Food Microbiology: Fundamentals and Frontiers. Washington, DC, USA: ASM Press; 2007. pp. 457–491.
Jay JM, Loessner MJ, Golden DA. Modern Food Microbiology. 7th ed. New York, NY, USA: Springer Science and Business Media; 2005.
Croonenbergs RE. Contamination of shellfish-growing waters. In: Martin RE, Carter EP, Flick GJ Jr, Davis LM, editors. Marine and Freshwater Products Handbook. Lancaster, PA, USA: Technomic Publishing Company Inc.; 2000. pp. 665–693.
Rodas-Suárez OR, Flores-Pedroche JF, Betacourt-Rule JM, Quinõnes-Ramírez EI, Vazquez-Salinas C. Occurrence and antibiotic sensitivity of L. monocytogenes strains isolated from oysters, fish, and estuarine water. Appl Environ Microb 2006; 72: 7410–7412. de Simon M, Terrago C, Ferrer MD. Incidence of L. monocytogenes in fresh foods in Barcelona (Spain). Int J Food Microbiol 1992; 16: 153–156.
Monfort P, Minet J, Rocourt J, Piclet G, Cormier M. Incidence of Listeria spp. in Breton live shellfish. Lett Appl Microbiol 1998; 26: 205–208.
Jacquet C, Gouin E, Jeannel D, Cossart P, Rocourt J. Expression of ActA, Ami, InlB, and listeriolysin O in L. monocytogenes of human and food origin. Appl Environ Microb 2002; 68: 616– 6
Kathariou S. L. monocytogenes virulence and pathogenicity, a food safety perspective. J Food Prot 2002; 65: 1811–1829.
Doumith M, Buchrieser C, Glaser P, Jacquet C, Martin P. Differentiation of the major L. monocytogenes serovars by multiplex PCR. J Clin Microbiol 2004; 42: 3819–3822.
Chen Y, Knabel SJ. Multiplex PCR for simultaneous detection of the genus Listeria, Listeria minocytogenes, and major serotypes and epidemic clones of L. monocytogenes. Appl Environ Microb 2007; 73: 6299–6304.
Aureli P, Ferrini AM, Mannoni V, Hodzic S, Wedell-Weergaard C, Oliva B. Susceptibility of L. monocytogenes isolated from food in Italy to antibiotics. Int J Food Microbiol 2003; 83: 325–330.
European Food Safety Authority (EFSA). The European Union summary report on trends and sources of zoonoses, zoonotic agents, antimicrobial resistance and foodborne outbreaks in 20 EFSA J 2012; 10: 2597. de Cesare A, Manfreda G, Macri M, Cantoni C. Application of automated ribotyping to support the evaluation of the L. monocytogenes source in a Taleggio producing plant and to predict the risk human health linked to the accidental sale of contaminated cheese. Arch Vet Ital 2006; 57: 231–240 .
Antunes P, Reu C, Sousa JC, Pestana N, Peixe L. Incidence and susceptibility to antimicrobials agents of Listeria spp. and L. monocytogenes isolated from poultry carcasses in Porto, Portugal. J Food Prot 2002; 65: 1888–1893.
Yusel N, Citak S, Onder M. Prevalence and antibiotic resistance of Listeria species in meat products in Ankara, Turkey. Food Microbiol 2005; 22: 241–245.
Arslan S, Özdemir F. Prevalence and antimicrobial resistance of Listeria spp. in homemade white cheese. Food Control 2008; 19: 360–363.
International Organization for Standardization. Microbiology of food and animal feeding. Horizontal method for the detection and enumeration of L. monocytogenes. ISO 11290-1: 1996/
FDAM 1: 2004 (E). Geneva, Switzerland: ISO; 2004.
Lawrence LM, Gilmour A. Incidence of Listeria spp. and L. monocytogenes in a poultry processing environment and in poultry products and their rapid confirmation by multiplex PCR. Appl Environ Microb 1994; 60: 4600–4604.
Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing; 14th Informational Supplement M100–S14. Vol. 24, No 1. Wayne, PA, USA: CLSI; 2004.
El Marrakchi A, Boumhandi R, Hamama A. Performance of a new chromogenic plating medium for the isolation of L. monocytogenes from marine environments. Lett Appl Microbiol 2005; 40: 87–91.
Ferrer MD, de Simon M. Isolation of Salmonella spp., V. parahemolyticus, Y. enterocolytica and L. monocytogenes in bivalve mollusks. Alimentaria 1993; 30: 33–35.
Vernocchi P, Maffei M, Lanciotti R, Suzzi G, Gardini F. Characterization of Mediterranean mussels (Mytilus galloprovincialis) harvested in Adriatic Sea (Italy). Food Control 2007; 18: 1575–1583.
Bremer PJ, Fletcher GC, Osborne C. L. monocytogenes in seafood. Auckland, New Zealand: New Zealand Institute for Crop & Food Research Limited; 2003.
Brett MSY, Short P, McLauchlin J. A small outbreak of listeriosis associated with smoked mussels. Int J Food Microbiol 1998; 43: 223–229.
Inoue S, Nakama A, Arai Y, Kokubo Y, Maruyama T, Salto A, Yoshida T, Terao M, Yamamoto S, Kumagai S. Prevalence and contamination levels of L. monocytogenes in retail food in Japan. Int J Food Microbiol 2000; 59: 73–77.
Pinto AL, Teixeira P, Castilho F, Felicio MT, Pombal F, Eibbs PA. Prevalence and serotyping of L. monocytogenes in Portuguese live bivalve molluscs sampled in various steps along the sanitary control process. Aquac Res 2006; 37: 1112–1116.
Kueh CSW, Chan K. Bacteria in bivalve shellfish with special reference to the oyster. J Appl Bacteriol 1985; 59: 41–47.
International Commission on Microbiological Specifications for Foods. Microorganisms in Foods 6. Microbial Ecology of Food Commodities. 2nd ed. New York, NY, USA: Kluwer Academic; 200 Aarestrup FM. Other pathogens. In: Aarestrup FM, editor. Antimicrobial Resistance in Bacteria of Animal Origin. Washington, DC, USA: ASM Press; 2006. pp. 249–267.