Isolation of Streptococcus species from the tonsils of slaughtered pigs

The palatine tonsils of 220 pigs were examined for the occurrence of Streptococcus suis and other Streptococcus species. Samples were cultured onto Colombia agar plates with 5% defibrinated horse blood and selective antibiotic supplement and were incubated at 37 °C for 24-48 h aerobically. Presumptive colonies were identified using API20 Strep (bioMérieux). S. suis was not detected in any of the 220 palatine tonsils examined. S. porcinus was the most common streptococcus recovered and was isolated from 68 (31%) pigs. From the samples, S. agalactiae was isolated from 19 (8.6%), S. equi subsp. zooepidemicus from 10 (4.5%), S. uberis from 6 (2.7%), S. dysgalactiae subsp. equisimilis from 4 (1.8%), Enterococcus faecium from 4 (1.8%), and S. pyogenes from 1 (0.5%). The absence of S. suis among the pigs examined is hopeful. However, further studies on this disease and other infectious diseases should continue. Additionally, this is the first report of the isolation of S. porcinus in swine in Turkey.

Isolation of Streptococcus species from the tonsils of slaughtered pigs

The palatine tonsils of 220 pigs were examined for the occurrence of Streptococcus suis and other Streptococcus species. Samples were cultured onto Colombia agar plates with 5% defibrinated horse blood and selective antibiotic supplement and were incubated at 37 °C for 24-48 h aerobically. Presumptive colonies were identified using API20 Strep (bioMérieux). S. suis was not detected in any of the 220 palatine tonsils examined. S. porcinus was the most common streptococcus recovered and was isolated from 68 (31%) pigs. From the samples, S. agalactiae was isolated from 19 (8.6%), S. equi subsp. zooepidemicus from 10 (4.5%), S. uberis from 6 (2.7%), S. dysgalactiae subsp. equisimilis from 4 (1.8%), Enterococcus faecium from 4 (1.8%), and S. pyogenes from 1 (0.5%). The absence of S. suis among the pigs examined is hopeful. However, further studies on this disease and other infectious diseases should continue. Additionally, this is the first report of the isolation of S. porcinus in swine in Turkey.

___

  • Katsumi, M., Kataoka, Y., Takahasmi, T., Kikuchi, N., Hiramune, T.: Bacterial isolation from slaughtered pigs associated with endocarditis, especially the isolation of Streptococcus suis. J. Vet. Med. Sci., 1997; 59: 75–78.
  • Katsumi, M., Kataoka, Y., Takahasmi, T., Kikuchi, N., Hiramune, T.: Biochemical and serological examination of beta-haemolytic streptococci isolated from slaughtered pigs. J. Vet. Med. Sci., 1998; 60: 129–131.
  • Chanter, N., Jones, P.W., Alexander, T.J.L.: Meningitis in pigs caused by Streptococcus suis — a speculative review. Vet. Microbiol., 1993; 36: 39–55.
  • McCaw, M.B.: Streptococcus suis: Nagging nursery nightmare. Proceedings of the North Carolina Healthy Hogs Seminar, North Carolina Swine Veterinary Group; 1993.
  • Staats, J.J., Feder, I., Okwumabua, O., Chengappa, M.M.: Streptococcus suis: past and present. Vet. Res. Commun., 1997; 21: 381–407.
  • Wisselink, H.J., Reek, F.H., Vecht, U., Stochofe-Zurwieden, N., Smits, M.A., Smith, H.E.: Detection of virulent strains of Streptococcus suis type 2 and highly virulent strains of Streptococcus suis type 1 in tonsillar specimens of pigs by PCR. Vet. Microbiol., 1999; 67: 143–157.
  • Madsen, L.W., Aalbaek, B., Nielsen, O.L., Jensen, H.E.: Aerogenous infection of microbiologically defined minipigs with Streptococcus suis serotype 2. A new model. APMIS, 2001; 109: 412–418.
  • Arends, J.P., Hartwig, N., Rudolphy, M., Zanen, H.C.: Carrier rate of Streptococcus suis capsular type 2 in palatine tonsils of slaughtered pigs. J. Clin. Microbiol., 1984; 20: 945–947. Aarestrup, F.M., Jorsal, S.E., Jensen, M.E.: Serological characterization and antimicrobial susceptibility of Streptococcus suis isolates from diagnostic samples in Denmark during 1995 and 1996. Vet. Microbiol., 1998; 60: 59–66. Higgins, R., Gottschalk, M.: Streptococcal diseases. In: Straw, B.E., Allaire, S., Mengeling, W.L., Taylor, D.J., Eds. Diseases of Swine. 8th edn., Iowa University Press, Iowa, USA. 1999; 563–578.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Animal skeletal remains of the Theodosius harbor: general overview

Vedat ONAR, Gülsün PAZVANT, Hasan ALPAK, Nazan GEZER İNCE, Altan ARMUTAK, Zeynep Sevim KIZILTAN

Isolation of Streptococcus species from the tonsils of slaughtered pigs

Arzu Funda BAĞCIGİL, Serkan İKİZ, Kemal METİNER, Naciye Yakut ÖZGÜR, Seyyal AK, Ahmet Atila ILGAZ

Financial losses from foot diseases in cattle farms in Kars Province

Pınar DEMİR, Sadık YAYLA, Özgür AKSOY, İsa ÖZAYDIN

Histological changes in the bone structure induced at 12 weeks by experimental administration of bisphosphonates

Horea Artimoniu ALMAŞAN, Mihaela BACIUT, Oana Cristina ALMAŞAN, Simion BRAN, Grigore BACIUT

Effect of different transport temperatures on in vitro maturation of oocytes collected from frozen-thawed sheep ovaries

Özen Banu ÖZDAŞ, Alper BARAN, Muzaffer TAŞ, Ümüt CİRİT, Kamber DEMİR, Süleyman BACINOĞLU, Serhat PABUCCUOĞLU, Kemal AK

Effects of feeding dry fat and yeast culture on broiler chicken performance

Mutassim Mohamed ABDELRAHMAN

Determination of Newcastle disease virus among wild bird populations in Lake Van basin, Turkey

Banur BOYNUKARA, Timur GÜLHAN, Fethiye ÇÖVEN, İlhami KİZİROĞLU, Atilla DURMUŞ

Intrascrotal testicular torsion and seminoma in a dog with chronic renal failure

Leandro Zuccolotto CRIVELLENTI, Tathiana Ferguson MOTHEO, Ricardo Lima SALOMAO, Daniel KAN HONSHO, Claudia MOMO

Susceptibility of Prototheca zopfii strains isolated from cows with mastitis to chlorhexidine and iodine

Henryk KRUKOWSKI, Andrzej LISOWSKI, Bozena NOWAKOWICZ-DEBEK, Lukasz WLAZLO

Comparative imaging of the vesicular glands in New Zealand white rabbits (Oryctolagus cuniculus)

Rosen DIMITROV, Kamelia STAMATOVA, Dimitar KOSTOV