Ayşe KILIÇ, Adile MUZ, Hasan Basri ERTAŞ, Gökben ÖZEY

Isolation of Arcanobacterium (Actinomyces) pyogenes from abscessed catle kidney and identification by PCR

Bu çalışmada, deri, eklem ve iç organlarda çeşitli prulent infeksiyonlara sebep olan Arcanobacterium spp.'nin apseli sığır böbreklerindeki varlığı araştırıldı. Toplam 500 sığır postmortem olarak muayene edildi ve apseli olan 100 örnek toplandı. Arcanobacterium pyogenes 40 (% 40) numuneden izole edildi. Izolatlardan elde edilen DNA'lar A. pyogenes'in plo geninden türetilen spesifik primerler kullanılarak Polimeraz Zincir Reaksiyonunda (PZR) çoğaltıldılar ve tüm izolatların PZR'da pozitif olduğu saptandı. Bu sonuç çalışmada kullanılan PZR yönteminin A. pyogenes'in identifikasyonunda uygulanabileceğini göstermiştir.

Apseli sığır böbreklerinden Arcanobacterium (Actinomyces) pyogenes izolasyonu ve PCR ile identifikasyonu

In this study, the presence of Arcanobacterium spp., which causes a variety of purulent infections involving the skin, joints and visceral organs, was investigated in abscessed kidney samples of cattle. A total of 500 cattle were examined at postmortem and 100 samples with abscess were collected. Arcanobacterium pyogenes was isolated in 40 (40%) of the samples examined. DNA extracted from the isolates were amplified by Polymerase Chain Reaction (PCR) using specific primers derived from plo gene of A. pyogenes and all the isolates were determined to be positive by PCR. It was concluded that PCR employed in this study may be applied for identification of A. pyogenes.

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