Asuman ÖZEN, Özge ÖZGENÇ, Ahmet CEYLAN, İrem SANCAK GÜL

Isolation of adipose tissue-derived stem cells

Mesenchymal stem cells are being used increasingly in cell-based therapies. Adipose tissue is an important source of mesenchymal stem cells and is also routinely used in research on lipid metabolism and obesity. Their high expansion potential and the ease of isolation make these cells an attractive cell source for regenerative therapies. The objective of this review is to give detailed information about the isolation, expansion, and clinical use of these cells in veterinary medicine.

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1. Gartner LP, Hiatt JL. Color Textbook of Histology. 1st ed. Philadelphia, PA, USA: Saunders; 1997.
2. Huang SJ, Fu RH, Shyu WC, Liu SP, Jong GP, Chiu YW, Wu HS, Tsou YA, Cheng CW, Lin SZ. Adipose-derived stem cells: isolation, characterization, and differentiation potential. Cell Transplant 2013; 22: 701709.
3. Nae S, Bordeianu I, Stăncioiu AT, Antohi N. Human adipose-derived stem cells: definition, isolation, tissue-engineering applications. Rom J Morphol Embryol 2013; 54: 919924.
4. Dhurandhar NV. A framework for identification of infections that contribute to human obesity. Lancet Infect Dis 2011; 11: 963969.
5. Salehian B, Forman SJ, Kandeel FR, Bruner DE, He J, Atkinson RL. Adenovirus 36 DNA in adipose tissue of patient with unusual visceral obesity. Emerg Infect Dis 2010; 16: 850852.
6. Csaki C, Matis U, Mobasheri A, Ye H, Shakibaei M. Chondrogenesis, osteogenesis and adipogenesis of canine mesenchymal stem cells: a biochemical, morphological and ultrastructural study. Histochem Cell Biol 2007; 128: 507520.
7. Özen A, Gül Sancak İ. Mezenkimal kök hücreler ve veteriner hekimlikte kullanımı. Ankara Üniv Vet Fak Derg 2014; 61: 7984 (in Turkish).
8. Sancak İG, Özen A, Bayraktaroğlu AG, Ceylan A, Can P. Characterization of mesenchymal cells isolated from the adipose tissue of young and old dogs. Ankara Üniv Vet Fak Derg (in press).
9. Riis S, Zachar V, Boucher S, Vemuri MC, Pennisi CP, Fink T. Critical steps in the isolation and expansion of adipose-derived stem cells for translational therapy. Expert Rev Mol Med 2015; 17: e11.
10. Gül Sancak İ, Özen A, Alparslan Pınarlı F, Tiryaki M, Ceylan A, Acar U, Delibaşı T. Limbal stem cells in dogs and cats their identification culture and differentiation into keratinocytes. Kafkas Üni Vet Fak Derg 2014; 20: 909914.
11. Ozen A, Gul Sancak I, Koch S, Von Rechenberg B. Ultrastructural characteristics of sheep and horse mesenchymal stem cells (MSCs). Mic Res 2013; 1: 1723.
12. Özen A, Gül Sancak İ, Tiryaki M, Ceylan A, Alparslan Pınarlı F, Delibaşı T. Mesenchymal stem cells (Mscs) in scanning electron microscopy (SEM) world. Niche 2013; 2: 2224.
13. Kolaparthy LK, Sanivarapu S, Moogla S, Kutcham RS. Adipose tissue - adequate, accessible regenerative material. Int J Stem Cells 2015; 8: 121127.
14. Clynes M. Cell culture models for study of differentiated adipose cells. Stem Cell Res Ther 2014; 5: 137.
15. Patrikoski M, Juntunen M, Boucher S, Campbell A, Vemuri MC, Mannerström B, Miettinen S. Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cells. Stem Cell Res Ther 2013; 4: 27.
16. Baer PC. Adipose-derived mesenchymal stromal/stem cells: an update on their phenotype in vivo and in vitro. World J Stem Cells 2014; 6: 256265.
17. Kuo YR, Chen CC, Goto S, Lee IT, Huang CW, Tsai CC, Wang CT, Chen CL. Modulation of immune response and T-cell regulation by donor adipose-derived stem cells in a rodent hind-limb allotransplant model. Plast Reconstr Surg 2011; 128: 661e672e.
18. Niemeyer P, Vohrer J, Schmal H, Kasten P, Fellenberg J, Suedkamp NP, Mehlhorn AT. Survival of human mesenchymal stromal cells from bone marrow and adipose tissue after xenogenic transplantation in immunocompetent mice. Cytotherapy 2008; 10: 784795.
19. Puissant B, Barreau C, Bourin P, Clavel C, Corre J, Bousquet C, Taureau C, Cousin B, Abbal M, Laharrague P et al. Immunomodulatory effect of human adipose tissue-derived adult stem cells: comparison with bone marrow mesenchymal stem cells. Br J Haematol 2005; 129: 118129.
20. Niemeyer P, Kornacker M, Mehlhorn A, Seckinger A, Vohrer J, Schmal H, Kasten P, Eckstein V, Sudkamp NP, Krause U. Comparison of immunological properties of bone marrow stromal cells and adipose tissue-derived stem cells before and after osteogenic differentiation in vitro. Tissue Eng 2007; 13: 111121.
21. Kang JM, Han M, Park IS, Jung Y, Kim SH, Kim SH. Adhesion and differentiation of adipose-derived stem cells on a substrate with immobilized fibroblast growth factor. Acta Biomater 2012; 8: 17591767.
22. Park IS, Han M, Rhie JW, Kim SH, Jung Y, Kim IH, Kim SH. The correlation between human adipose-derived stem cells differentiation and cell adhesion mechanism. Biomaterials 2009; 30: 68356843.
23. Marble HD, Sutermaster BA, Kanthilal M, Fonseca VC, Darling EM. Gene expression-based enrichment of live cells from adipose tissue produces subpopulations with improved osteogenic potential. Stem Cell Res Ther 2014; 5: 145.
24. Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 2001; 7: 211228.
25. Lettry V, Hosoya K, Takagi S, Okumura M. Co-culture of equine mesenchymal stem cells and mature equine articular chondrocytes results in improved chondrogenic differentiation of the stem cells. Jpn J Vet Res 2010; 58: 515.
26. Koerner J, Nesic D, Romero JD, Brehm W, Mainil-Varlet P, Grogan SP. Equine peripheral blood- derived progenitors in comparison to bone marrow- de-rived mesenchymal stem cells. Stem Cells 2006; 24: 16131619.
27. Vieira NM, Brandalise V, Zucconi E, Secco M, Strauss BE, Zatz M. Isolation, characterization, and differentiation potential of canine adipose-derived stem cells. Cell Transplantation 2010; 19: 279289.