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Immunohistochemical Localization of FSH Cells in the Pars Distalis of the Pituitary Gland in Five-Month-Old Laying Hens

The present study was performed to determine the structural differences and immunohistochemical distribution of follicle-stimulating hormone (FSH) producing cells in the pars distalis region of the pituitary glands of 5 month-old laying hens, using the labeled avidin-biotin method. Hens begin to lay eggs at approximately the age of 5 months. It was observed that gonadotrophic cells containing FSH were present in both the cephalic and caudal zones and were densely distributed throughout the pars distalis. They appeared to be arranged in either cords or follicles in which branching and anastomosing occurred as well as being found as separate cells. The strongest positive FSH immunoreactivity was observed in most of the cells located in the caudal and cephalic zones of the pars distalis in 5 month-old laying hens. Furthermore, the total numbers of FSH immunoreactive cells (in 1 µm2) were similar in the cephalic and caudal zones.

Anahtar Kelimeler:

Pituitary gland, pars distalis, FSH cells, laying hens

Immunohistochemical Localization of FSH Cells in the Pars Distalis of the Pituitary Gland in Five-Month-Old Laying Hens

Keywords:

Pituitary gland, pars distalis, FSH cells, laying hens,

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Rahmanian, M.S., Thompson, D.L. Jr., Melrose, P.A.: Immunocytochemical localization of luteinizing hormone and follicle-stimulating hormone in the pituitary. J. Anim. Sci., 1998; 76: 839-846.
Dacheux, F.: Subcellular localization of gonadotropic hormones in pituitary cells of the castrated pig with the use of pre- and post- embedding immunocyotochemical methods. Cell Tissue Res., 1984; 236: 153-160.
Naik, D.R., Sar, M., Stumpf, W.E.: Immunohistochemical identification of cells in the pars distalis of the pituitary of the lizard, Anolis carolinensis. Histochemistry, 1980; 69: 19-26.
Pelletier, G., Leclerc, R., Fabrie, F.: Identification of gonadotropic cells in the human pituitary by immunoperoxidase technique. Mol. Cell. Endocrinol., 1976; 6: 123-128.
Proudman, J.A., Vandesande, F., Berghman, L.R: Immunohistochemical evidence that follicle-stimulating hormone and luteinizing hormone reside in separate cells in the chicken pituitary. Biol. Reprod., 1999; 60: 1324-1328.
Mikami, S.: Avian Hypophysis: Recent progress in immunocytochemical studies. In: Mikami, S., Homma, K., Wada, M. (eds), Avian Endocrinology: Environmental and Ecological Perspectives. New York, Springer-Verlag: 39-56, 1983.
Iwama, Y.: Immunohistochemical identification of mouse adenohypophysial gonadotropes. Okajimas Folia Anat. Jpn., 1990; 67: 281-287.
Mikami, S., Kurosu, T., Farner, D.S.: Light and electron microscopic studies on the secretory cytology of the adenohypophysis of the Japanese quail, Coturnix coturnix japanica. Cell Tissue Res., 1975; 159: 147-165.