Immunohistochemical lacolization of FSH cells in the pars distalis of the pituitary gland in five-month-old laying hans

Bu çalışmada, beş aylık yumurtacı tavukların FSH salgılayan gonodotrop hücreleri işaretli avidin-biotin yöntemi ile saptanarak; hücrelerin yapısal özellikleri ve pars distalisteki dağılımları incelendi. Beş aylık yaş, tavukların yumurtlama periyodunun başlangıcıdır. Pars distalisin sefhalik ve kaudal bölgelerinin her ikisinde de FSH immunreaktif hücreler görüldü. FSH-ir hücrelerin tek tek yerleşim gösterdiği gibi kordonlar, foliküller oluşturarak dallanmalar ve anostomozlar şekillendirdiği saptandı. Beş aylık yumurtacı tavukların pars distalisinde bulunan FSH-ir hücrelerin büyük çoğunluğunun kuvvetli pozitif immunreaksiyon verdiği gözlendi. Ayrıca FSH-ir hücrelerin toplam sayısı (1 µm2) hem sefhalik hem de kaudal bölgede birbirine oldukça yakın değerlerde bulundu.

Beş aylık tavukların hipofiz bezinin pars distalisindeki FSH içeren hücrelerin immunohistokimyasal dağılımı

The present study was performed to determine the structural differences and immunohistochemical distribution of follicle-stimulating hormone (FSH) producing cells in the pars distalis region of the pituitary glands of 5 month-old laying hens, using the labeled avidin-biotin method. Hens begin to lay eggs at approximately the age of 5 months. It was observed that gonadotrophic cells containing FSH were present in both the cephalic and caudal zones and were densely distributed throughout the pars distalis. They appeared to be arranged in either cords or follicles in which branching and anastomosing occurred as well as being found as separate cells. The strongest positive FSH immunoreactivity was observed in most of the cells located in the caudal and cephalic zones of the pars distalis in 5 month-old laying hens. Furthermore, the total numbers of FSH immunoreactive cells (in 1 µm2) were similar in the cephalic and caudal zones

PDF

___

1. Dellmann, H.D., Eurell, A.: Textbook of Veterinary Histology. Fifth Edition, Williams & Wilkins, USA, 1998.
2. Hattori, A., Ishii, S., Wada, M.: Different mechanisms controlling FSH and LH release in Japanese quail (Coturnix coturnixjaponica): Evidence for an inherently spontaneous release and production of FSH. J. Endocrinol., 1986; 108: 239-245.
3. Crossmonn, G.: A modification of Mallory's connective tissue stain with a discussion of the principles involved. Anat. Rec, 1937:69:33-38.
4. Erdost, H.: Immunohistochemical study on the chicken adenohypophysis using monoclonal mouse anti-human FSH as a primary antibody. Anat. Histol. Embryol., 2004; 33: 49-52.
5. Sümbüloğlu, K., Sümbüloğlu, U.: Biyoistatistik. Özdemir Yayıncılık, Ankara. 1994.
6. Farner, D.S., King, J.R., Parkes, K.C.: Avian Biology. Vol. Ill, Acedemic Press, New York 128-148, 1973.
7. Hodges, R.D.: The Histology of the Fowl, Academic Press, London, 429-431, 1974.
8. Bastings, E., Beckers, A., Reznik, M., Beckers, J.F.: Immunocytochemical evidence for production of luteinizing hormone and follicle-stimulating hormone in separate cells in the bovine. Biol. Reprod., 1991; 45: 788-796.
9. Pinelli, C, Fiorentino, M., D'Aniello, B., Tanaka, S., Rastogi, R.K.: Immunohistochemical demonstration of FSH and LH in the pituitary of the developing frog, Rana esculenta. Gen. Comp. Endocrinol. 1996; 104: 189-196.
10. Rahmanian, M.S., Thompson, D.L. Jr., Melrose, P.A.: Immunocytochemical localization of luteinizing hormone and follicle-stimulating hormone in the pituitary. J. Anim. Sci., 1998; 76: 839-846.
11. Dacheux, F.: Subcellular localization of gonadotropic hormones in pituitary cells of the castrated pig with the use of pre- and post- embedding immunocyotochemical methods. Cell Tissue Res., 1984;236: 153-160.
12. Naik, D.R., Sar, M., Stumpf, W.E.: Immunohistochemical identification of cells in the pars distaiis of the pituitary of the lizard, Anolis carolinensis. Histochemistry, 1980; 69: 19-26.
13. Pelletier, G., Leclerc, R., Fabrie, F.: Identification of gonadotropic cells in the human pituitary by immunoperoxidase technique. Mol. Cell. Endocrinol., 1976; 6: 123-128.
14. Proudman, J.A., Vandesande, F., Berghman, L.R: Immunohistochemical evidence that follicle-stimulating hormone and luteinizing hormone reside in separate cells in the chicken pituitary. Biol. Reprod., 1999; 60: 1324-1328.
15. Mikami, S.: Avian Hypophysis: Recent progress in immunocytochemical studies. In: Mikami, S., Homma, K., Wada, M. (eds), Avian Endocrinology: Environmental and Ecological Perspectives. New York, Springer-Verlag: 39-56, 1983.
16. Iwama, Y.: Immunohistochemical identification of mouse adenohypophysial gonadotropes. Okajimas Folia Anat. Jpn., 1990:67:281-287.
17. Mikami, S., Kurosu, T., Farner, D.S.: Light and electron microscopic studies on the secretory cytology of the adenohypophysis of the Japanese quail, Coturnix coturnix japanica. Cell Tissue Res., 1975; 159: 147-165.