Hematologic, biochemical, genetic, and histological biomarkers for the evaluation of the toxic effects of fipronil for Rhamdia quelen
Hematologic, biochemical, genetic, and histological biomarkers for the evaluation of the toxic effects of fipronil for Rhamdia quelen
This study aimed to determine the lethal concentration 50% (LC50) and behavioral, hematological, biochemical,histopathological, and genetic disorders of fipronil for Rhamdia quelen in acute toxicity tests for 96 h. To determine the LC50, 42 juvenileswere distributed in groups of 0, 0.1, 0.2, 0.3, 0.4, 0.5, and 0.8 mg L–1 of fipronil. For other evaluations 36 silver catfish were divided intocontrol, 0.3 mg L–1, and 0.4 mg L–1 groups. Blood, kidneys, livers, and gills were collected for evaluation. The LC50 calculated with probittyperegression was 0.811 mg L–1 at 48 h of study. There was total mortality of 0.8 mg L–1, reduction in hematocrit values, and increase inliver enzymes. Liver samples showed cytoplasmic vacuolization and cellular degeneration, among other changes. Gills presented vascularcongestion, complete fusion of secondary lamellae, and epithelial cell hypertrophy. In the kidneys, changes such as Bowman’s capsuleclearance, tubular degeneration, and glomerular capillary dilatation, among others, were common. Erythrocytes showed morphologicalalterations without increased micronucleus development. Fipronil induces a clinical condition of anemia, alterations in liver enzymelevels, nuclear erythrocyte changes, and liver, gill and kidney damage in the silver catfish.
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- 1. Ramsdorf W. Avaliação da toxicidade dos compostos fipronil,
nitrato de chumbo e naftaleno em peixes. MSc, Universidade
Federal do Paraná, Curitiba, Brazil, 2011 (in Portuguese).
- 2. Borges A, Scotti LV, Siqueira DR, Zanini R, Amaral F, Jurinitz
DF, Wassermann GF. Changes in hematological and serum
biochemical values in jundiá Rhamdia quelen due to sub-lethal
toxicity of cypermethrin. Chemosphere 2007; 69: 920-926.
- 3. Montanha FP, Fredianelli AC, Wagner R, Sacco SR, Rocha
DCC, Pimpão CT. Clinical, biochemical and haematological
effects in Rhamdia quelen exposed to cypermethrin. Arq Bras
Med Vet Zootec 2014; 66: 697-704.
- 4. Lermen CL, Lappe R, Crestani M, Vieira VP, Gioda CR,
Schetinger MRC, Baldisserotto B, Moraes G, Morsch VM.
Effect of different temperature regimes on metabolic and blood
parameters of silver catfish Rhamdia quelen. Aquaculture 2004;
239: 497-507.
- 5. Pimpão CT, Zampronio AR, Assis HCS. Effects of deltamethrin
on hematological parameters and enzymatic activity in
Ancistrus multispinis (Pisces, Teleostei). Pestic Biochem
Physiol 2007; 88: 122-127.
- 6. Galeb LAG, Fredianelli AC, Montanha FP, Mikos JD, Fan
ALPD, Rocha RMVM, Kirschinik P, Weber S, Pimpão CT.
Determination of lethal and sublethal concentrations of
deltamethrin in jundiá (Rhamdia quelen). Rev Acad Ciên
Anim 2013; 11: 125-130.
- 7. Albinati ACL, Moreira ELT, Albinati RCB, Carvalho JV, Lira
AD, Vidal LVO. Histological biomarkers – Chronic toxicity for
roundup in piauçu (Leporinus macrocephalus). Arq Bras Med
Vet Zootec 2009; 61: 621-627 (in Portuguese with an English
abstract).
- 8. Culling CFA, Allison RT, Barr WT. Cellular Pathology
Techniques. 4th ed. London, UK: Butterworths; 1985.
- 9. Heddle JA. A rapid in vivo test for chromosome damage. Mutat
Res 1973; 18: 187-192.
- 10. Beggel S, Werner I, Connon RE, Geist J. Sublethal toxicity
of commercial insecticide formulations and their active
ingredients to larval fathead minnow (Pimephales promelas).
Sci Total Environ 2010; 408: 3169-3175.
- 11. Beggel S, Connon R, Werner I, Geist J. Changes in gene
transcription and whole organism responses in larval fathead
minnow (Pimephales promelas) following short-term exposure
to the synthetic pyrethroid bifenthrin. Aquat Toxicol 2011; 105:
180-188.
- 12. Montanha FP, Galeb LAG, Mikos JD, Ganeco LN, Pereira TP,
Tanaka A, Kirschinik P, Pimpao CT. Pyrethroid toxicity in
silver catfish, Rhamdia quelen. Pesqui Vet Bras 2012; 32: 1297-
1303.
- 13. PAN-UK. Fipronil. London, UK: Pesticide Action Network
UK; 2012.
- 14. Velisek J, Jurcikova J, Dobsikova R, Svobodova Z, Piackova
V, Machova J, Novotny L. Effects of deltamethrin on rainbow
trout (Oncorhynchus mykiss). Environ Toxicol Pharmacol
2007; 23: 297-301.
- 15. Lopes STA, Biondo APS, Santos AP. Manual de Patologia
Clínica Veterinária. 3rd ed. Santa Maria, Brazil: Universidade
Federal de Santa Maria; 2007 (in Portuguese).
- 16. Pereira BF, Da Silva Alves RM, Pitol DL, Senhorini JA, De
Alcântara Rocha RCG, Caetano FH. Morphological gill
analysis of fish species Prochilodus lineatus after exposure to
pollutants. J Environment Analytic Toxicol 2012; 2: 1-4.
- 17. Thrall MA, Baker CD, Campbell TW, Denicola D, Fettman
MJ, Lassen ED, Rebar A, Weiser G. Hematologia e bioquímica:
Clínica veterinária. São Paulo, Brazil: Roca; 2007 (in
Portuguese).
- 18. Ghisi NC, Ramsdorf WA, Ferraro MVM, Almeida MIM,
Ribeiro CA, Cestari MM. Evaluation of genotoxicity in
Rhamdia quelen (Pisces, Siluriformes) after sub-chronic
contamination with Fipronil. Environ Monit Assess 2011; 180:
589-599.
- 19. Mela M, Guiloski IC, Doria HB, Randi MAF, Oliveira-Ribeiro
CA, Pereira L, Maraschi AC, Prodocimo V, Freire CA, Assis
HCS. Effects of the herbicide atrazine in neotropical catfish
(Rhamdia quelen). Ecotoxicol Environ Saf 2013; 93: 13-21.
- 20. Annabi A, Messaoudi I, Kerkeni A, Said KH. Cadmium
accumulation and histological lesion in mosquito fish
(Gambusia affinis) tissues following acute and chronic
exposure. Int J Environ Res 2011; 5: 745-756.
- 21. Silva AG, Martinez CBR. Morphological changes in the kidney
of a fish living in an urban streams. Environ Toxicol Pharmacol
2007; 23: 185-192.
- 22. Pamplona JH, Oba ET, Silva TA, Ramos LP, Ramsdorf WA,
Cestari MM, Ribeiro CAO, Zampronio AR, Assis HCS.
Subchronic effects of dipyrone on the fish species Rhamdia
quelen. Ecotoxicol Environ Saf 2011; 74: 342-334.
- 23. Bedor CNG, Ramos LO, Pereira PJ, Rêgo MAV, Pavão AC,
Augusto LGS. Vulnerability and risk situations related to the
use of pesticides in irrigated fruit farming. Rev Bras Epidemiol
2009; 12: 39-49 (in Portuguese with an English abstract).
- 24. Paz-y-Miño C, Sánchez ME, Arévalo M, Muñoz MJ, Witte T,
De-la-Carrera GO, Leone PE. Evaluation of DNA damage in an
Ecuadorian population exposed to glyphosate. Genet Mol Biol
2007; 30: 456-460.
- 25. Bolognese C, Hayashi M. Micronucleus assay in aquatic
animals. Mutagenesis 2011; 26: 205-213.