Gökkuşağı alabalıklarında (Oncorhynchus mykiss,walbaum) bazı immunostimulan'ların spesifik olmayan bağışıklık sistemi üzerine etkisi

Bu çalışmanın amacı, farklı oranlarda ß-glukan (MacroGard), Schizosaccharomyces pombe, S. pombe P450 içeren yemlerle ,beslenmenin gökkuşağı alabalıklarının (Oncorhynchus mykiss) spesifik olmayan savunma mekanizması üzerine etkisini belirlemektir. Bu amaçla balıklar, % 0,1 ve % l oranlarında MacroGard, % 0,1 ve % l oranlarında S. pombe, % 0,1 ve % 1 oranlarında S. pombe P450 içeren yemler ile üç hafta boyunca beslenmiştir. Beslemeden sonra fagositoz, intraselüler ve ekstraselüler respiratory burst ve plazma toplam protein miktarı, spesifik büyüme oranı, kondüsyon faktörü değerleri hesaplanmıştır. Üç farklı immunostknulan ile üç hafta besleme sonunda, ekstraselüler ve intraselüler respiratory burst ve fagositik aktivitenin, % l MacroGard ve % 0,1 S. pombe P450 ilave edilmiş yemler ile beslenen gökkuşağı alabalıklarında, kontrol grubuna ve diğer gruplara oranla önemli ölçüde yüksek olduğu görülmüştür (P < 0,05). Bütün gruplarda kontrol grubuna oranla yüksek respiratory burst aktivitesi görülmüştür. Fagositik aktivite % 0,1 MacroGard ile beslenen grup dışında kontrol grubuna oranla yüksek olarak kaydedilmiştir (P < 0,05). Plazma toplam protein miktarı bütün gruplarda kontrol grubuna oranla yüksek çıkmasına rağmen istatiksel olarak bir farklılığa rastlanmamıştır (P < 0,05). Sonuç olarak, deneysel koşullarda farklı dozlarda MacroGard, S. pombe ve S. pombe P450 ilave edilmiş yemler ile beslenen gökkuşağı alabalıklarının intraselüler ve ekstraselüler respiratory burst aktivitesi ve fagositoz gibi spesifik olmayan bağışıklık sistem parametrelerini artırdığı, ancak plazma protein miktarı üzerine % l MacroGard ve % 0,1 S. pombe P450 ile beslenen gruplar dışında etkili olmadığı görülmüştür. S. pombe P450 ticari bir ürün olan MacroGard kadar iyi spesifik olmayan bağışık yanıt oluşturduğu görülmüştür.

The effect of some immunostimulants on the non-specific immune system of rainbow trout (Oncorhynchus mykiss,walbaum)

The aim of this study was to determine the effect on non-specific defence mechanisms in rainbow trout (Oncorhynchus mykiss) fed diets containing different doses of ß-glucan (MacroGard), Schizosaccharomyces pombe and S. pombe P450. Over a period of 3 weeks, fish were given 0.1% and 1% MacroGard, 0.1% and 1% S. pombe, 0.1% and 1 % S. pombe P450 in their food. Then phagocytosis, intracellular and extracellular respiratory burst activity, total protein, and specific growth ratio and condition factor values were determined. After feeding for 3 weeks with three different immunostimulants, extracellular and intracellular respiratory burst and phagocytic activity were significantly increased in the groups fed 1% MacroGard and 0.1% S. pombe P450 compared with the control group and the other experimental groups (P < 0.05). Respiratory burst activity was higher in all the experimental groups than in the control group. Phagocytic activity was elevated in all the experimental groups except that fed 0.1 % MacroGard (P < 0.05). Total protein levels were higher in all the experimental groups than in the control group, but the difference was not significant (P < 0.05). The results indicate that rainbow trout fed different doses of MacroGard, S. pombe and S. pombe P450 all showed increased intracellular and extracellular respiratory burst activity, and phagocytic activity. However, there was no effect on total protein. In conclusion, S. pombe P450 could confer the same non-specific immunity as MacroGard.

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