Gökkuşağı alabalıklarında (Oncorhynchus mykiss) yem karbonhidat oranının yem alımı, nutrient kullanımı ve plazma glukozu üzerine etkileri

A twelve-week nutrition trial was carried out to evaluate the efficacy of different carbohydrate levels on digestibility, feed intake, growth performance, carcass and muscle composition and plasma glucose level in the rainbow trout, Oncorhynchus mykiss. Three test diets were formulated using extruded wheat meal (15.3, 32.2 and 43.5%) as the carbohydrate source and fed rainbow trout (Initial Body Weight: 33.8$pm$ 0.46 g.). These were either restricted: LCR (Low Carbohydrate Restricted), MCR (Medium-Carbohydrate-Restricted) and HCR (High-Carbohydrate Restricted); or satiation: LCS (Low-Carbohydrate Satiation), MCS (Medium-Carbohydrate Satiation) and HCS (High-Carbohydrate Satiation) respectively. The aim of applying different feeding regimes was to quantify the protein sparing effect of carbohydrate and to determine the response of rainbow trout to different levels of carbohydrates. The results showed that protein, energy and carbohydrate digestibility was reduced with increasing dietary carbohydrate levels, whilst lipid digestibility was similar all the groups. There was a significant energy substitution from the carbohydrate source in the restricted groups. However, since the maximum feeding rate was reached in the restricted regimes (MCR and HCR), a similar sparing action of carbohydrate was observed in the satiation groups (MCS and HCS). All the groups displayed good growth performance, and exhibited growth that was LCS exhibited the highest (P< 0.05) growth rate. MCR and MCS trout exhibited growth that was superior (P< 0.05) to that of the LCR, HCR and HCS treatments. The feed efficiency of all the treatments was close to 100%. The quantities of carcass and muscle protein, lipid and ash were not found to be different (P> 0.05). Transient hyperglycaemia was observed in trout fed a high carbohydrate diet. These results are discussed with respect to the protein sparing action of dietary carbohydrate in practical trout diets and the possible physiological effects of carbohydrates on feed intake regulation.

Dietary carbohydrate level influencing feed intake, nutrient utilization and plasma glucose concentration in the rainbow trout, Oncorhynchus mykiss

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1.National Research Council: Nutrient requirements of fish. NationalAcademy of Sciences. 1993: Washington, D. C.
2.Wilson, R. P.: Utilization of dietary carbohydrate by fish.Aquaculture. 1994: 124: 67-80.
3.Phillips, A. M., Tunison, A. V. and Brockway, D. R.: The utilizationof carbohydrates by trout. Fisheries Research Bulletin. 1948: No:11. New York State Conversation Department, Bureau of FishCulture, Albany, N. Y.
4.Luquet, P.: Efficacite des proteines en relation avec leurs tauxd’incorporation dans l’alimentation de la truite arc-en-ciel. Ann.Hydrobiol. 1971: 2: 175-186.
5.Cowey, C. B., De La Higuera, M. and Adron, J. W.: The effect ofdietary composition and of insulin on gluconeogenesis in rainbowtrout (Salmo gairdneri). Br. J. Nutr. 1977a: 38: 385-395.
6.Cowey, C. B., Knox, D., Walton, M. J. and Adron, J. W.: Theregulation of gluconeogenesis by diet and insulin in rainbow trout(Salmo gairdneri). Br. J. Nutr. 1977b: 38: 463-470.
7.Kaushik, S. J. and Teles, A. O.: Effect of digestible energy onnitrogen and energy balance in rainbow trout. Aquaculture. 1985:50: 89-101.
8.Kaushik, S. J., Medale, F., Fauconneau, B. and Blanc, D.: Effect ofdigestible carbohydrates on protein / energy utilization and onglucose metabolism in Rainbow trout (Salmo gairdneri R.).Aquaculture. 1989: 79: 63-74.
9.Steffens, W.: Principles of Fish Nutrition, Ellis Horwood, London.1989: 383 p.
10. Kim, J. D. and Kaushik, S. J.: Contribution of digestible energyfrom carbohydrates and estimation of protein / energyrequirements for growth of rainbow trout (Oncorhynchus mykiss).Aquaculture. 1992: 106: 161-169.
11. Tekinay, A. A.: Dietary Interactions Influencing feed intake, nutrientutilization and appetite regulation in the rainbow trout,Oncorhynchus mykiss. Ph.D Thesis, 1999. University of Plymouth,UK.
12. Austreng, E.: Digestibility determination in fish using chromicoxide marking and analysis of contents from different segments ofthe gastrointestinal tract. Aquaculture. 1978: 13: 265-272.
13. Folch, J., Lees, M. and Sloan-Stanley, G. H.: A simple method forthe isolation and purification of total lipids from animal tissues. J.Biol. Chem. 1959: 226: 497-509.
14. AOAC: Official Methods of Analysis, 15thedition (K. Helrich,editor). Arlington, Virginia, U.S.A. AOAC: 1990: 1298p.
15. Morris, P. C.: The water soluble vitamin nutrition of gilthead seabream, Sparus aurata. Ph.D Thesis. 1994. University of Plymouth,UK.
16. Furukawa, A. and Tsukahara, H.: On the acid digestion method forthe determination of chromic oxide as an index substance in thestudy of digestibility of fish feed. Bull. of the Japan. Soc. of Sci.Fish. 1966: 32, (6): 502.
17. Singh, R. P. and Nose, T.: Digestibility of carbohydrates in youngrainbow trout. Bull. Freshwater Fish. Res. Lab. 1967: 17, (1): 21-25.
18. Steel, R. G. D. and Torrie, J. H.: Principles and procedures ofstatistics: a biometric approach, second edition. McGraw Hill, NewYork. 1960.
19. Shearer, K. D.: Factors affecting the proximate composition ofcultured fishes with emphasis on salmonids. Aquaculture. 1994:119: 63-88.
20. Inaba, D., Ogino, C., Takamatsu, C., Ueda, T. and Kurokawa, K.:Digestibility of dietary components in fishes. 2. Digestibility ofdietary protein and starch in rainbow trout. Bull. Jpn. Soc. Sci.Fish. 1963: 29: 242-244.
21. Rychly, J. and Spannhof, L.: Nitrogen balance in trout. I.Digestibility of diets containing varying levels of protein andcarbohydrate. Aquaculture. 1979: 16: 39-46.
22. Takeuchi, T., Jeong, K. S. and Watanabe, T.: Availability ofextruded carbohydrate ingredients to rainbow trout (Oncorhynchusmykiss) and carp (Cyprinus carpio). Nipp. Suis. Gak.. 1990: 56:1839-1845.
23. Furuichi, M. and Yone, Y.: Effect of dietary dextrin levels on thegrowth and feed efficiency, the chemical composition of liver anddorsal muscle and the absorption of dietary protein and dextrin infishes. Bull. Japan. Soc. Sci. Fish. 1980: 46: 225-229.
24. Spannhof, L. and Plantikow, H.: Studies on carbohydrate digestionin rainbow trout. Aquaculture. 1983: 30: 95-108.
25. Bergot, F. and Breque, J.: Digestibility of starch by rainbow trout:effects of the physical state of starch and of the intake level.Aquaculture. 1983: 34: 203-212.
26. Bergot, F.: Digestibility of native starches of various botanicalorigins by rainbow trout (Oncorhynchus mykiss). In: Fish Nutritionin Practice (Editors: Kaushik S. J. and Luquet P.), Proceedings ofthe IVthInternational Symposium on Fish Nutrition and Feeding,June 24-27, 1991, Biarritz, France. INRA editions. 1993: 857-865 p.
27. Henrichfreise, B. and Pfeffer, E.: Wheat and wheat starch aspotential sources of digestible energy for rainbow trout(Oncorhynchus mykiss). J. Anim. Physiol. a. Anim. Nutr. 1992:67: 143-147.
28. Pfeffer, E. and Henrichfreise, B.: Evaluation of potential sources ofprotein in diets for rainbow trout (Oncorhynchus mykiss). Arch.Anim. Nutr. 1994: 45: 371-377.
29. Liou, B. and Nagayama, F.: Properties of pyruvate kinase from carphepatopancreas and rainbow trout liver. Bull. of the Jap. Soc. ofSci. Fish. 1986: 52, (12): 2189-2195.
30. Baeverfjord, G.: Digestible and indigestible carbohydrates inrainbow trout diets. Dr. Scient. Thesis. 1992. Norwegian Collegeof Veterinary Medicine, Norway.
31. Lee, D. J. and Putnam, G. B. The response of rainbow trout tovarying protein / energy ratios in a test diet. J. Nutr. 1973: 103:916-922.
32. Refstie, T. and Austreng, E.: Carbohydrate in rainbow trout diets.III. Growth and chemical composition of fish from different familiesfed four levels of carbohydrate in the diet. Aquaculture. 1981: 25:35-39.
33. Hilton, J. W. and Atkinson, J. L.: Response of Rainbow trout(Salmo gairdneri) to increased levels of available carbohydrate inpractical trout diets. Br. J. Nutr. 1982: 47: 597-607.
34. Hilton, J. W., Atkinson, J. L. and Slinger, S. J.: Evaluation of thenet energy value of glucose and maize starch in diets for rainbowtrout (Salmo gairdneri). Br. J. Nutr. 1987: 58: 453-461.
35. Hilton, J. W., Atkinson, J. L. and Slinger, S. J.: Effect of increaseddietary fiber on the growth of rainbow trout (Salmo gairdneri).Can. J. of Fish. and Aqua. Sci. 1983: 40: 81-85.
36. Cho C. Y. and Kaushik S. J.: Effects of protein intake onmetabolizable and net energy values of fish diets. In: C. B. Cowey,A. Mackie and J. Bell (Editors), Nutrition and Feeding in Fish.Academic Press, London. 1985: 95-117 p.
37.lliott, J. M.: The energetics of feeding, metabolism and growth ofbrown trout (Salmo trutta L.) in relation to body weight, watertemperature and ration size. J. Anim. Ecol. 1976: 45: 923-948.
38. Persson, L.: Food evacuation and models for multiple meals infishes. Env. Biol. Fishes. 1984: 10: 305-309.
39. Bromley, P.J.: The role of gastric evacuation experiments inquantifying the feeding rates of predatory fish. Rev. in Fish Biol.and Fish. 1994: 4: 36-66.
40. Austreng, E., Risa, S., Edwards, D. J. and Hvidsten, H.:Carbohydrate in rainbow trout diets. 2. Influence of carbohydratelevels on chemical composition and feed utilization of fish fromdifferent families. Aquaculture. 1977: 11: 39-50.
41. Beamish, F. W. H., Hilton, J. W., Niimi, E. and Slinger, S. J.:Dietary carbohydrate and growth, body composition and heatincrement in rainbow trout (Salmo gairdneri). Fish Phys. &Biochem. 1986: 2: 85-91.
42. Bergot, F.: Carbohydrate in Rainbow trout diets: effects of the leveland source of carbohydrate and the number of meals on growthand body composition. Aquaculture. 1979: 18: 157-167.
43. Walton, M. J.: Metabolic effects of feeding a high protein / lowcarbohydrate diet as compared to a low protein / highcarbohydrate diet to rainbow trout, Salmo gairdneri. Fish Phys.and Biochem.. 1986: 1, (1): 7-15.
44. Grove, D. J., Loizides, L. G. and Nott, J.: Satiation amount,frequency of feeding and gastric emptying rate in Salmo gairdneri.J. Fish Biol. 1978: 12: 507-516.
45. Sheridan, M. A. and Mommsen, T. P.: Effects of nutritional stateon in vivo lipid and carbohydrate metabolism of coho salmon,Oncorhynchus kisutch. Gen. and Comp. Endocr. 1991: 81: 473-483.
46. Harmon, J. S. and Sheridan, M. A.: Effects of nutritional state,insulin and glucagon on lipid mobilization in rainbow trout,Oncorhynchus mykiss. Gen. and Comp. Endocr. 1992: 87: 214-221.
47. Himick, B. A. and Peter, R. E.: Neuropeptide regulation of feedingand growth hormone secretion in fish. Neth. J. Zool. 1995: 45,(1-2): 3-9.
48. MacKenzie, D. S., Vanputte, C. M. and Leiner, K. A.: Nutrientregulation of endocrine function in fish. Aquaculture. 1998: 161:3-25