Effects of rumen-protected methionine and lysine on milk yield and milk composition in Holstein dairy cows consuming a corn grain and canola meal-based diet

Methionine (Met) and lysine (Lys) are thought to be the two most important amino acids for lactation performance. Met and Lys dietary supplementation can thus be an effective approach to improve amino acid balance for lactation performance, particularly in early lactation. The purpose of this study was to determine how rumen-protected (RP) Met + Lys affect milk production, milk composition, and feed efficiency in primiparous Holstein dairy cows fed a corn grain and canola meal-based diet for 60 days (8.5 weeks) following calving. Two hundred primiparous Holstein dairy cows were randomly assigned to one of two dietary treatments based on their expected calving date: 1) a basal diet; or 2) a basal diet supplemented with RP Met + Lys. Milk production and feed consumption were tracked on a daily basis, and milk components were tested once a week. During the trial, drinking water was always available in front of the dairy cows. The RP Met + Lys considerably increased (p < 0.05) milk yield (+ 2.20 kg/d), fat corrected milk yield (+ 2.18 kg/d) and feed efficiency but had no effect on dry matter intake (p > 0.05). There was no effect of treatment on either starting or final body weight (p > 0.05). Cows receiving RP Met + Lys, on the other hand, showed a tendency (p = 0.09) of reduced body condition score (BCS) losses between weeks 1 and 8. The RP Met + Lys increased (p < 0.05) the proportion of milk fat, but had no effect on the other milk composition markers (p > 0.05). Except for milk crude protein, the yields of fat, true protein, lactose, and energy in milk were higher (p < 0.05) in cows fed RP Met + Lys vs. control cows. The results indicate that supplementing with RP Met + Lys after the first calving has a considerable impact on subsequent milk and milk fat production, as well as the yield of the majority of milk nutrients.

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1. National Research Council (NRC). Nutrient Requirements of Dairy Cattle. 7th rev. ed. ed. Natl. Acad. Sci., Washington, DC. 2001.
2. Spears RA, Kohn RA, Young AJ. Whole-farm nitrogen balance on western dairy farms. Journal of Dairy Science 2003; 86: 4178-4186.
3. Nadeau E, Englund JE, Gustafsson AH. Nitrogen efficiency of dairy cows as affected by diet and milk yield. Livestock Science 2007; 111: 45-56.
4. Lee C, Hristov AN, Cassidy TW, Heyler KS, Lapierre H et al. Rumen-protected lysine, methionine, and histidine increase milk protein yield in dairy cows fed a metabolizable proteindeficient diet. Journal of Dairy Science 2012; 95: 6042-6056.
5. Broderick GA, Stevenson MJ, Patton RA. Effect of dietary protein concentration and degradability on response to rumenprotected methionine in lactating dairy cows. Journal of Dairy Science 2009; 92: 2719-2728.
6. Schwab CG. Rumen-protected amino acids for dairy cattle: Progress towards determining lysine and methionine requirements. Animal Feed Science & Technology 1996; 59: 87-101.
7. Liu YG, Peng HH, Schwab CG. Enhancing the productivity of dairy cows using amino acids. Animal Production Science 2013; 53: 1156-1159.
8. Socha MT, Putnam DE, Garthwaite BD, Whitehouse NL, Kierstead NA et al. Improving intestinal amino acid supply of pre- and postpartum dairy cows with rumen-protected methionine and lysine. Journal of Dairy Science 2005; 88: 1113-1126.
9. Noftsger S, St-Pierre NR, Sylvester JT. Determination of rumen degradability and ruminal effects of three sources of methionine in lactating cows. Journal of Dairy Science 2005; 88: 223-237.
10. Třináctý J, Křížová L, Richter M, Černý V, Říha J. Effect of rumen-protected methionine, lysine or both on milk production and plasma amino acids of high-yielding dairy cows. Czech Journal of Animal Science 2009; 54: 239-248.
11. Lee C, Lobos NE, Weiss WP. Effects of supplementing rumenprotected lysine and methionine during prepartum and postpartum periods on performance of dairy cows. Journal of Dairy Science 2019; 102: 11026-11039.
12. Zhao K, Liu W, Lin XY, Hu ZY, Yan ZG et al. Effects of rumen-protected methionine and other essential amino acid supplementation on milk and milk component yields in lactating Holstein cows. Journal of Dairy Science 2019; 102: 7936-7947.
13. Batistel F, Arroyo JM, Bellingeri A, Wang L, Saremi B et al. Ethyl-cellulose rumen-protected methionine enhances performance during the periparturient period and early lactation in Holstein dairy cows. Journal of Dairy Science 2017; 100: 7455-7467.
14. Garthwaite BD, Schwab CG, Sloan BK. Amino acid nutrition of the early lactation cow. Cornell Nutrition Conference for Feed Manufacturers 1998; pp 38-50.
15. Grummer RR. Impact of changes in organic nutrient metabolism on feeding the transition dairy cow. Journal of Animal Science 1995; 73: 2820-2833.
16. Broderick GA, Faciola AP, Armentano LE. Replacing dietary soybean meal with canola meal improves production and efficiency of lactating dairy cows. Journal of Dairy Science 2015; 98: 5672-5687.
17. Huhtanen P, Hetta M, Swensson C. Evaluation of canola meal as a protein supplement for dairy cows: A review and a metaanalysis. Canadian Journal of Animal Science 2011; 91: 529-543.
18. Lavergne S, Oleson F. Canada: Principal Field Crops Supply and Disposition. Stat Canada 2018; pp 1-11.
19. Martineau R, Ouellet DR, Lapierre H. The effect of feeding canola meal on concentrations of plasma amino acids. Journal of Dairy Science 2014; 97: 1603-1610.
20. Paula EM, Broderick GA, Danes MAC, Lobos NE, Zanton GI et al. Effects of replacing soybean meal with canola meal or treated canola meal on ruminal digestion, omasal nutrient flow, and performance in lactating dairy cows. Journal of Dairy Science 2018; 101: 328-339.
21. Association of Official Analytical Chemists (AOAC). Official methods of analysis of the association of official analytical chemists, 15th ed.. Arlington, VA: Association of Official Analytical Chemists.1990.
22. Tyrrell HF, Reid JT. Prediction of the energy value of cow’s milk. Journal of Dairy Science 1965; 48: 1215-1223.
23. Swanepoel N, Robinson PH, Conley A. Impacts of substitution of canola meal with soybean meal, with and without ruminally protected methionine, on production, reproduction and health of early lactation multiparous Holstein cows through 160 days in milk. Animal Feed Science & Technology 2020; 264: 114494.
24. Drackley JK. Biology of dairy cows during the transition period: The final frontier? Journal of Dairy Science 1999; 82: 2259-2273.
25. Osorio JS, Ji P, Drackley JK, Luchini D, Loor JJ. Smartamine M and MetaSmart supplementation during the peripartal period alter hepatic expression of gene networks in 1-carbon metabolism, inflammation, oxidative stress, and the growth hormone-insulin-like growth factor 1 axis pathways. Journal of Dairy Science 2014; 97: 7451-7464.
26. Wang C, Liu HY, Wang YM, Yang ZQ, Liu JX et al. Effects of dietary supplementation of methionine and lysine on milk production and nitrogen utilization in dairy cows. Journal of Dairy Science 2010; 93: 3661-3670.
27. Awawdeh MS. Rumen-protected methionine and lysine: Effects on milk production and plasma amino acids of dairy cows with reference to metabolisable protein status. Journal of Dairy Research 2016; 83: 151-155.
28. Overton TR, Waldron MR. Nutritional management of transition dairy cows: Strategies to optimize metabolic health. Journal of Dairy Science 2004; 87: E105-E119.
29. Piepenbrink MS, Marr AL, Waldron MR, Butler WR, Overton TR et al. Feeding 2-hydroxy-4-(methylthio)-butanoic acid to periparturient dairy cows improves milk production but not hepatic metabolism. Journal of Dairy Science 2004; 87: 1071- 1084.
30. Volden H, Velle W, Harstad OM, Aulie A, Sjaastad ØV. Apparent ruminal degradation and rumen escape of lysine, methionine, and threonine administered intraruminally in mixtures to high-yielding cows. Journal of Animal Science 1998; 76: 1232-1240.
31. Fehlberg LK, Guadagnin AR, Thomas BL, Sugimoto Y, Shinzato I et al. Feeding rumen-protected lysine prepartum increases energy-corrected milk and milk component yields in Holstein cows during early lactation. Journal of Dairy Science 2020; 103: 11386-11400.
32. Rulquin H, Graulet B, Delaby L, Robert JC. Effect of different forms of methionine on lactational performance of dairy cows. Journal of Dairy Science 2006; 89: 4387-4394.
33. Phipps RH, Reynolds CK, Givens DI, Jones AK, Geraert PA et al. Short communication: Effects of 2-hydroxy-4-(methylthio) butanoic acid isopropyl ester on milk production and composition of lactating holstein dairy cows. Journal of Dairy Science 2008; 91: 4002-4005.
34. Robinson PH. Impacts of manipulating ration metabolizable lysine and methionine levels on the performance of lactating dairy cows: A systematic review of the literature. Livestock Science 2010; 127: 115-126.
35. Ordway RS, Boucher SE, Whitehouse NL, Schwab CG, Sloan BK. Effects of providing two forms of supplemental methionine to periparturient Holstein dairy cows on feed intake and lactational performance. Journal of Dairy Science 2009; 92: 5154-5166.
36. Gil LA, Shirley RL, Moore JE. Effect of methionine hydroxy analog on growth, amino acid content, and catabolic products of glucolytic rumen bacteria in vitro. Journal of Dairy Science 1973; 56: 757-762.
37. Patton RA, McCarthy RD, Griel LC. Observations on rumen fluid, blood serum, and milk lipids of cows fed methionine hydroxy analog. Journal of Dairy Science 1970; 53: 776-780.
38. Xu S, Harrison JH, Chalupa W, Sniffen C, Julien W et al. The effect of ruminal bypass lysine and methionine on milk yield and composition of lactating cows. Journal of Dairy Science 1998; 81: 1062-1077.
39. Larsen M, Lapierre H, Kristensen NB. Abomasal protein infusion in postpartum transition dairy cows: Effect on performance and mammary metabolism. Journal of Dairy Science 2014; 97: 5608-5622.