Effects of Double Injections of PGF2a at Different Intervals on Some Reproductive Traits in Tuj Ewes

The present study investigated the effects of double injections of PGF2a at different intervals (10 vs. 14 days) on plasma progesterone levels, oestrus and lambing rates in Tuj ewes during the breeding season. Thirty clinically healthy mature ewes were assigned randomly into 2 experimental groups: 2 injections of 125 µg of Cloprostenol (a PGF2a analogue) at either 10 (Group I, n = 15) or 14 day (Group II, n = 15) intervals. Blood samples were collected from the jugular vein on the day of the second injection of PGF2a, 4 days post-injection and 16 days after insemination to determine plasma progesterone levels. The oestrus and lambing rates were also determined. Findings showed that double injections of PGF2a at 10 or 14 day intervals did not significantly affect either the concentrations of plasma progesterone or the rates of oestrus and lambing. Plasma progesterone concentrations on the day of the second injection, 4 days post-injection and 16 days after insemination were 4.29 ± 1.96, 0.27 ± 0.19 and 7.39 ± 3.5 ng/ml in group I and 3.45 ± 1.45, 0.10 ± 0.02 and 9.21 ± 4.29 ng/ml in group II, respectively. The oestrus and lambing rates were 86.7% and 60.2% in group I, and 100% and 73.3% in group II, respectively. In conclusion, it appears that for oestrus synchronisation, shortening the interval of double injections of PGF2a by 4 days has little effect on plasma progesterone concentrations, oestrus or lambing rates in Tuj ewes during the breeding season.

Effects of Double Injections of PGF2a at Different Intervals on Some Reproductive Traits in Tuj Ewes

The present study investigated the effects of double injections of PGF2a at different intervals (10 vs. 14 days) on plasma progesterone levels, oestrus and lambing rates in Tuj ewes during the breeding season. Thirty clinically healthy mature ewes were assigned randomly into 2 experimental groups: 2 injections of 125 µg of Cloprostenol (a PGF2a analogue) at either 10 (Group I, n = 15) or 14 day (Group II, n = 15) intervals. Blood samples were collected from the jugular vein on the day of the second injection of PGF2a, 4 days post-injection and 16 days after insemination to determine plasma progesterone levels. The oestrus and lambing rates were also determined. Findings showed that double injections of PGF2a at 10 or 14 day intervals did not significantly affect either the concentrations of plasma progesterone or the rates of oestrus and lambing. Plasma progesterone concentrations on the day of the second injection, 4 days post-injection and 16 days after insemination were 4.29 ± 1.96, 0.27 ± 0.19 and 7.39 ± 3.5 ng/ml in group I and 3.45 ± 1.45, 0.10 ± 0.02 and 9.21 ± 4.29 ng/ml in group II, respectively. The oestrus and lambing rates were 86.7% and 60.2% in group I, and 100% and 73.3% in group II, respectively. In conclusion, it appears that for oestrus synchronisation, shortening the interval of double injections of PGF2a by 4 days has little effect on plasma progesterone concentrations, oestrus or lambing rates in Tuj ewes during the breeding season.

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  • Laçin, E, Aksoy, A.R.: Kars bölgesinde yetifltirilen Morkaraman ve Tuj koyunlarının döl verimi özelliklerinin karflılafltırılması. Kafkas Univ. Vet. Fak. Derg., 2003; 9: 5-7.
  • Taylor, R.E., Field, T.G.: Scientific Farm Animal Production. Prentice Hall, Upper Saddle River, NJ, USA. 2001; 198-212.
  • Öztürkler, Y., Çolak, A., Baykal, A., Güven, B.: Combined effect of a prostaglandin analogue and a progestagen treatment for 5 days on oestrus synchronisation in Tushin ewes. Indian Vet. J., 2003; 80: 917-920.
  • Haresign, W.: Manipulation of reproduction in sheep. J. Reprod. Fertil. Suppl., 1992; 45: 127-139.
  • Yıldız, S., Saatci, M., Uzun, M., Guven, B.: Effects of ram introduction after the second prostaglandin F2alpha injection on day 11 on the LH surge characteristics in fat-tailed ewes. Reprod. Domest. Anim., 2003; 38: 54-57.
  • Henderson, D.C., Downing, J.M., Beck, N.F.G., Lees, J.L.: Estrus synchronization in ewes: a comparison of prostaglandin F2αTham salt with a progestagen pessary. Anim. Product., 1984; 39: 229- 233.
  • Beck, N.F.G., Davies, M.C.G., Davies, B., Lees, L.: Estrus
  • synchronization and fertility in ewes: A comparison of three methods. Anim. Product., 1987; 44: 251-254.
  • Beck, N.F.G., Jones, M., Davies, B., Peters, A.R., Williams, S.P.: Estrus synchronization in ewes: the effect of combining a prostaglandin analogue with a GnRH agonist (buserelin). J. Anim. Sci., 1996; 62: 85-87.
  • Kaya, İ, Öncüer, A, Ünal, Y, Yıldız, S.: Nutritive value of pastures in Kars district I. Botanical and nutrient composition at different stages of maturity. Turk. J. Vet. Anim. Sci., 2004; 28: 275-280.
  • Katz, L.S., McDonald, T.J.: Sexual behaviour of farm animals. Theriogenology, 1992; 38: 239-253.
  • Ptaszynska, M.: Compendium of Animal Reproduction. 6th Revised edn. Intervet Int. bv (The Netherlands). 2001; 125-147.
  • Prakash, B.S., Meyer, H.H.D., Schallenberger, E., van de Wiel, D.F.M.: Development of sensitive EIA for progesterone determination in unextracted bovine plasma using the second antibody technique. J. Steroid. Biochem., 1987; 28: 623-627.
  • Meyer, H.H.D., Guven, B., Karg, H.: Enzymimmuntest (EIA) auf mikrotitration Platten zur progesteronbestimmung in Magermilchproben. Wien. Tierarztl. Monat., 1986; 73: 86-94.
  • Meyer, H.D.D., Guven, B.: Improvement of microtitration plate enzymeimmunoassay for steroid determination by a “second antibody technique”. J. Steroid. Biochem., 1986; 25: 139-143.
  • İleri, İ.K.: Koyunlarda bir prostoglandin F2αanaloğu olan Tiaprost (İliren) ile östrus sinkronizasyonu ve suni tohumlama çalıflmaları. İstanbul Üniv. Vet. Fak. Derg., 1985; 11: 15-30.
  • Jainudeen, M.R., Hafez, E.S.E.: Reproductive failure in females. In Hafez E.S.E., Ed. Reproduction in Farm Animals. 5th edn. Lea & Febiger, Philadelphia, USA. 1987; 399-422.
  • Tekeli, T., Aksoy, M., Semecan, A., Karaca, F., Ayar, A.: Estrus and pregnancy rates of Konya Merino ewes treated with a double injection of cloprostenol at different intervals. Arch. Tierz. Dummerstorf, 1997; 40: 57-60.
  • Horoz, H., Ak, K., Kaflıkçı, G., Baran, A., Sönmez, C., fienünver, A., İleri, İ.K.: Üreme mevsiminde östrus sinkronizasyon yöntemleri uygulanan kıvırcık koyunlarında serum progesteron, östradiol 17βve LH seviyeleri. Kafkas. Üniv. Vet. Fak. Derg., 1997; 3: 85-92.
  • Godfrey, R.W., Collins, J.R., Hensley, E.L., Wheaton, J.E.: Estrus synchronization and artificial insemination of hair sheep ewes in the tropics. Theriogenology, 1999; 51: 985-997.
  • Hackett, A.J., Robertson, H.A.: Effect of dose and time of injection of prostaglandin F2αin cycling ewes. Theriogenology, 1980; 13: 347-351.
  • Gökçen, H., Ünal, E.F., Tümen, H., Deligözoğlu, F., Soylu, M.K., Çelik, İ.: Kızgınlıkları değiflik yöntemler ile sinkronize edilerek tohumlanan Merinos koyunlarında döl verimi üzerinde arafltırmalar. Uludağ Üniv. Vet. Fak. Derg., 1992; 2: 81-112.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
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