Namık DELİBAŞ, Mehmet AKDOĞAN, Murat KANBUR, Gökhan ERASLAN, Bilal Cem LİMAN

Effects of dietary aflatoxin and hydrate sodium calcium aluminosilicate on triiodothyronine, thyroxine, thyrotrophin and testosterone levels in quails

Çalışma, 80 adet, erkek Coturnix coturnix japonica ırkı, 14 günlük bıldırcınlar üzerinde gerçekleştirildi. Bıldırcınlar, biri kontrol diğer yedisi deneme olmak üzere sekiz gruba ayrıldı ve her bir grupta 10 hayvan bulunduruldu. Kontrol grubuna ticari rasyon verilirken deneme gruplarından Grup 2, 3, 4, 5, 6, 7 ve 8'in yemlerine sırasıyla 2,5 g/kg yem HSCAS; 5,0 g/kg yem HSCAS; 10,0 g/kg yem HSCAS; 2,50 ppm aflatoksin (Af B1 % 78,30, Af B2 % 14,60, Af G1 % 4,50, Af G2 % 2,60); 2,50 ppm AF+2,50 g/kg yem HSCAS; 2,50 ppm AF+5,0 g/kg yem HSCAS; 2,50 ppm AF+10,0 g/kg yem HSCAS katıldı ve 21 gün süreyle verildi. Denemenin sonunda, hayvanlardan kan alındı ve kanda triiodotronin (T3), tiroksin (T4), tirotropin (TSH) ve testosteron düzeyleri ölçüldü. Sonuçta, kontrol grubuna göre istatistiksel olarak, T3 düzeyinde Grup 3 ve 4'de önemli bir artış, Grup 5 ve 6'da önemli bir düşüş; T4 düzeyinde Grup 2, 4, 5, 7 ve 8'de önemli bir artış; testosteron düzeyinde bütün deneme gruplarında (Grup 2-8) önemli bir düşüş tespit edildi.

Bıldırcınlarda triiodotronin, tiroksin, tirotropin ve testosteron düzeyleri üzerine yemle verilen aflatoksin ve hidrate sodyum kalsiyum alüminosilikatın etkisi

This study was performed on 80 male 14-day-old Coturnix coturnix japonica breed quails. The quails were divided into 8 groups with 10 animals in each as 1 control and 7 trial groups. While the control group was fed a commercial basal ration, groups 2-8 received, respectively, 2.5 g/kg feed hydrate sodium calcium aluminosilicate (HSCAS), 5.0 g/kg feed HSCAS, 10.0 g/kg feed HSCAS, 2.5 ppm aflatoxin (AF B1 78.30%, AF B2 14.60%, AF G1 4.50%, AF G2 2.60%); 2.5 ppm AF with 2.5 g/kg feed HSCAS, 2.5 ppm AF with 5.0 g/kg feed HSCAS, and 2.5 ppm AF with 10.0 g/kg feed HSCAS, respectively, for 21 days. At the end of the trial, blood samples were taken from the animals and triiodothyronine (T3), thyroxine (T4), thyrotrophin (TSH) and testosterone levels in the blood were measured. Statistically significant increases were detected in T3 levels in groups 3 and 4 and significant decreases in groups 5 and 6, while there were significant increases in T4 levels in groups 2, 4, 5, 7 and 8 and significant decreases in blood testosterone levels in all trial (groups 2 to 8) groups compared to the control (group 1).

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1. Gugnani, H.C.: Ecology and taxonomy of pathogenic aspergilli. Front. Biosci., 2000; 8: 346-357.
2. Robens, J.F., Richard, J.L.: Aflatoxins in animal and human health. Rev. Environ. Contam. Toxicol., 1992; 127: 69-94.
3. Ostrowski-Meissner, H.T.: Effect of contamination of diets with aflatoxins on growing ducks and chickens. Trop. Anim. Health Prod., 1983; 15: 161-168.
4.Parlat, S.S., Ozcan, M., Oğuz, H.: Biological suppression of aflatoxicosis in Japanese quail (Coturnix coturnix japonica) by dietary addition of yeast (Saccharomyces cerevisiae). Res. Vet. Sci., 2001; 71: 207-211.
5.Parlat, S.S., Yildiz, A.O., Oguz, H.: Effect of clinoptilolite on performance of Japanese quail {Coturnix coturnix japonica) during experimental aflatoxicosis. Br. Poult. Sci., 1999; 40: 495-500.
6. Dalvi, R.R.: An overview of aflatoxicosis of poultry: its characteristics, prevention and reduction. Vet. Res. Commun., 1986; 10:429-443
7. Kaya, S.: Mikotoksinler. İçinden: Kaya, S., Pirincci, I., Bilgili, A. ed: Veteriner Hekimliğinde Toksikoloji. Medisan Yayın Serisi 53, Ankara, 544-573, 2002.
8. Pier, A.C., Richard, J.L., Cysewski, S.J.: Implications of mycotoxins in animal disease. J. Am. Vet. Med. Assoc, 1980; 176:719-724.
9. Ortatatli, M., Oguz, H.: Ameliorative effects of dietary clinoptilolite on pathological changes in broiler chickens during aflatoxicosis. Res. Vet. Sci., 2001; 71: 59-66.
10. Keçeci, T., Oguz, H., Kurtoglu, V., Demet; 0.: Effects of polyvinylpolypyrrolidone, synthetic zeolite and bentonite on serum biochemical and haematological characters of broiler chickens during aflatoxicosis. Br. Poult. Sci., 1998; 39: 452-458.
11. Harvey, R.B., Kubena, L.F., Phillips, T.D.: Evaluation of aluminosilicate compounds to reduce aflatoxin residues and toxicity to poultry and livestock: a review report. Sci. Total Environ., 1993; Suppl. Pt. 2: 1453-1457.
12. Marquez Marquez, R.N., Tejada de Hernandez, I.: Aflatoxin adsorbent capacity of two Mexican aluminosilicates in experimentally contaminated chick diets. Food Addit. Contam., 1995; 12: 431-433.
13. Diaz, D.E., Hagler, W.M. Jr, Hopkins, B.A., Whitlow, LW.: Aflatoxin binders I: in vitro binding assay for aflatoxin Bl by several potential sequestering agents. Mycopathologia, 2002; 156:223-226. 14. Demet, Ö., Oğuz, H., Çelik, L, Nizamlıoğlu, F.: Pirinçte aflatoksin üretilmesi. Vet. Bil. Derg., 1995; 11: 19-23.
15. Shotwell, O.L., Hesseltine, C.W., Stubblefield, R.D., Sorenson, W.G.: Production of aflatoxin on rice. Appl. Microbiol., 1966; 14: 425-428.
16. Roberts, B.A., Patterson, D.S.P.: Detection of twelve mycotoxins in mixed animal feedstuffs, using a novel membrane cleanup procedure. J. Assoc. Off. Anal. Chem., 1975; 58: 1178-1181.
17. Şanlı, Y., Ceylan, S., Kaya, S.: Karma yemlerde aflatoksin analizi. Ankara Üniv. Vet. Fak. Derg., 1982; 29: 50-70.
18. Nabney, j., Nesbit, B.F.: A spectrophotometric method for determination of the aflatoxins. Analyst, 1965; 3: 155-159.
19. Nakamura, H., Nakao, K.: Mechanism of regulation of TSH-biosynthesis and secretion. Nippon Rinsho., 1993; 51: 2611-2617.
20. Rose, S.R.: Disorders of thyrotropin synthesis, secretion, and function. Curr. Opin. Pediatr., 2000; 12: 375-381.
21. Oguz, H., Kurtoglu, V.: Effect of clinoptilolite on performance of broiler chickens during experimental aflatoxicosis. Br. Poult. Sci., 2000;41:512-517.
22. Wiersinga, W.M.: Nuclear thyroid hormone receptors. Neth. J. Med., 1985:28:74-82.
23. Noyan, A.: Yaşamda ve Hekimlikte Fizyoloji. Hormonlar, 977-1033, 1993, Ankara.
24. Markou, K., Georgopoulos, N., Kyriazopoulou, V., Vagenakis, A.G.: Iodine-induced hypothyroidism. Thyroid, 2001; 11: 501-510.
25. Dunn, J.T., Dunn, A.D.: The importance of thyroglobulin structure for thyroid hormone biosynthesis. Biochimie, 1999; 81: 505-509.
26. Rastogi, R., Srivastava, A.K., Rastogi, A.K.: Long term effect of aflatoxin Bl on lipid peroxidation in rat liver and kidney: effect of picroliv and silymarin. Phytother. Res., 2001; 15: 307-310.
27. Grosicki, A., Kowalski, B.: Influence of bentonite on trace element kinetics in rats. Bull. Vet. Inst. Pulawy, 2003; 47: 555-558.
28.Aydiner, A., Aytekin, Y., Topuz, E.: Effects of cisplatin on testicular tissue and the Leydig cell-pituitary axis. Oncology, 1997; 54: 74-78.
29. Ortatatli, M., Ciftci, M.K., Tuzcu, M., Kaya, A.: The effects of aflatoxin on the reproductive system of roosters. Res. Vet. Sci., 2002; 72: 29-36.