Zülfikar Kadir SARITAŞ, Nusret APAYDIN, Eser ÖZGENCİL, Kamuran PAMUK, A. Tulga ULUS

Effects of ATP-MgCl2 on Myocardial Ischemia-Reperfusion Injury: An In Vivo Experimental Study

We investigated the effect of ATP-MgCl2 on myocardial hemodynamics and metabolism. The study included 12 mongrel dogs that were randomly divided into 2 groups: the control and the ATP-MgCl2 (study) groups. Following a right thoracotomy, the left anterior descending artery was ligated for 15 min and then reperfusion was performed. After the ischemic period, cardiopulmonary bypass was initiated and the aorta was cross-clamped. ATP-MgCl2 (70+70 mmol/l) 0.25 ml/kg per h was given to the study group at the start of the ischemic period. Hemodynamic and metabolic measurements were obtained at basal, and then after 60, 120, and 180 min of reperfusion Cardiac output values were significantly higher in the study group; at the end of the third hour of reperfusion they were 2370 ± 31 ml/min in the study group and 900 ± 45 ml/min in the control group (P < 0.05). Myocardial Oxygen Extraction (MOE) and Myocardial Lactate Extraction (MLE) values were significantly higher in the control group; at the end of the third hour of the reperfusion MOE values were 50 ± 2 ml in the study group and 55 ± 3 ml in the control group, while MLE values were -0.06 ± 0.02 mmol/ml in the study group and -0.12 ± 0.07 mmol/ml in the control group (P < 0.05). Hemodynamic and metabolic parameters revealed that the use of ATP-MgCl2 might be beneficial in reducing ischemia-reperfusion damage.

Anahtar Kelimeler:

In vivo experimental study, ischemia-reperfusion injury, myocardium, ATP-MgCl2

Effects of ATP-MgCl2 on Myocardial Ischemia-Reperfusion Injury: An In Vivo Experimental Study

Keywords:

In vivo experimental study, ischemia-reperfusion injury, myocardium, ATP-MgCl2,

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Kirklin, J.W., Conti, V.R., Blackstone, E.H.: Prevention of myocardial damage during cardiac operations. N. Engl. J. Med., 1979; 301: 135-141.
Jennings, R.B., Reimer, K.A.: Lethal myocardial ischemic injury. Am. J. Pathol., 1981; 102: 241-255.
Demopoulos, H.B., Flamm, E.S., Pietronigro, D.D., Seligman, M.L.: The free radical pathology and the microcirculation in the major central nervous system disorders. Acta Physiol. Scand. Suppl., 1980; 492: 91-119.
McCord, J.M.: Oxygen derived free radicals in postischemic tissue injury. N. Engl. J. Med., 1985; 312: 159-163.
Katırcıoğlu, S.F., Ulus, A.T., Sarıtafl, Z., Gökçe, P.: Effects of ATP-MgCl2administration in hypovolemic dogs. Panminerva Med., 1999; 41: 323-330.
Ulus, A.T., Sarıtafl, Z., Yamak, B., Sürücü, S., Tuncer, M., Katırcıoğlu, S.F.: ATP-MgCl2utilization for spinal cord protection during experimental thoracic aortic occlusion. J. Cardiovasc. Surg., 1999; 40: 495-499.
Cuevas, P., Reimers, D., Carceller, F., Jimenez, A.: Ischemic reperfusion injury in rabbit spinal cord: protective effect of superoxide dismutase on neurological recovery and spinal infarction. Acta. Anat., 1990; 137: 303-310.
Agee, J.M., Flanagan, T., Blackbourne, L.H., Kron, I.L., Tribble, C.G.: Reducing postischemic paraplegia using conjugated superoxide dismutase. Ann. Thorac. Surg., 1991; 51: 911-914.
Krause, G.S., White, B.C., Aust, S.D., Nayini, N.R., Kumar, K.: Brain cell death following ischemia and reperfusion: a proposed biochemical sequence. Crit. Care Med., 1988; 16: 714-726
Mauney, M.C., Blackbourne, L.H., Langenburg, S.E., Buchanan, S.A., Kron, I.L., Tribble, C.G.: Prevention of spinal cord injury after repair of the thoracic or thoracoabdominal aorta. Ann. Thorac. Surg., 1995; 59: 245-252.
Chaudry, I.H., Clemens, M.G., Baue, A.E.: The role of ATP- magnesium in ischemia and shock. Magnesium, 1986; 5: 211- 220.
Chaudry, I.H., Stephan, R.N., Dean, R.E., Clemens, M.G., Baue, A.E.: The use of magnesium-ATP following liver ischemia. Magnesium, 1988; 7: 68-77.
Simpson, J.I., Eide, T.R., Schiff, G.A., Clagnaz, J.F., Hossain, I., Tverskoy, A., Koski, G.: Intratechal magnesium sulfate protects the spinal cord from ischemic injury during thoracic aortic cross- clamping. Anesthesiology, 1994; 81: 1493-1499.