Suhail Salam MIR, Farooz Ahmad LONE, Mohammad Zaffarullah KHAN, Asloob Ahmad MALIK, Rafiqul ISLAM, Khursheed Ahmad SOFI

90112

Effect of cold storage period on the quality of ram cauda epididymal spermatozoa recovered postmortem

Postmortem spermatozoa recovery is an important tool for harvesting germplasm from genetically meritorious animals and endangered species. The principal motive of this study was to find out whether ram spermatozoa within epididymides kept at 4 °C for 48 h retain their quality in terms of progressive motility, viability, and acrosomal status. Twenty-four testicles with attached epididymides were collected with 8 samples each processed at 0, 24, and 48 h of storage. A good number of motile spermatozoa could be recovered from epididymides by incision method with up to 48 h of storage, although their quality declined significantly (P < 0.05) as postmortem storage time increased (motility: 83.33 ± 1.05%, 63.33 ± 2.47%, and 44.17 ± 1.53%; and epididymal sperm output (millions): 3500.00 ± 73.97, 1220.00 ± 1.01, and 666.67 ± 19.78 for 0 (control), 24, and 48 h, respectively). Viability and intact acrosomes (%) were also acceptable, although they decreased significantly with up to 24 h of storage. In conclusion, ram epididymides could be stored at 4 °C for 48 h when epididymal spermatozoa cannot be immediately harvested and cryopreserved.
Anahtar Kelimeler:

Key words: Cauda epididymides, spermatozoa, ram, cold storage

Effect of cold storage period on the quality of ram cauda epididymal spermatozoa recovered postmortem

Postmortem spermatozoa recovery is an important tool for harvesting germplasm from genetically meritorious animals and endangered species. The principal motive of this study was to find out whether ram spermatozoa within epididymides kept at 4 °C for 48 h retain their quality in terms of progressive motility, viability, and acrosomal status. Twenty-four testicles with attached epididymides were collected with 8 samples each processed at 0, 24, and 48 h of storage. A good number of motile spermatozoa could be recovered from epididymides by incision method with up to 48 h of storage, although their quality declined significantly (P < 0.05) as postmortem storage time increased (motility: 83.33 ± 1.05%, 63.33 ± 2.47%, and 44.17 ± 1.53%; and epididymal sperm output (millions): 3500.00 ± 73.97, 1220.00 ± 1.01, and 666.67 ± 19.78 for 0 (control), 24, and 48 h, respectively). Viability and intact acrosomes (%) were also acceptable, although they decreased significantly with up to 24 h of storage. In conclusion, ram epididymides could be stored at 4 °C for 48 h when epididymal spermatozoa cannot be immediately harvested and cryopreserved.
Keywords:

Key words: Cauda epididymides, spermatozoa, ram, cold storage,

PDF
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: 6
  • Yayıncı: TÜBİTAK
Arşiv
Sayıdaki Diğer Makaleler

Craniometric analysis of Early Medieval horses Equus przewalskii f. caballus (Linnaeus, 1758) from chosen areas in Poland

Edyta PASICKA, Aleksander CHRÓSZCZ, Maciej JANECZEK, Anna MUCHA

Ultrasonographic evaluation of Cricoarytenoideus lateralis muscle in Standardbred horse

Fulvio LAUS, Emanuele PAGGI, Matteo CERQUETELLA, Beniamino TESEI

Determination of egg consumption and consumer habits in Turkey

Cengizhan MIZRAK, İsmail DURMUŞ, Serdar KAMANLI, Şahnur ERDOĞAN DEMİRTAŞ, Süleyman KALEBAŞI, Ender KARADEMİR, Murat DOĞU

Ultrasound imaging, biochemical blood analyses, and weight investigations of dissectible fat depots in New Zealand white rabbits

Penka YONKOVA, Anton RUSENOV, Dean KANAKOV, Dimitrinka ZAPRYANOVA, Ekaterina VACHKOVA, Ayşe SERBEST, Rosen DIMITROV, Dimitar KOSTOV

Reproductive performance and serum concentrations of progesterone of heat-stressed Holstein heifers subjected to timed artificial insemination and progesterone supplementation

Abelardo CORREA CALDERÓN, Juan Augusto HERNÁNDEZ RIVERA, Leonel AVENDAÑO REYES, Ulises MACIAS CRUZ, Raúl DIAZ MOLINA, Francisco Daniel ALVAREZ VALENZUELA, Arnoldo GONZALEZ-REYNA

Prevention of predicted neonatal isoerythrolysis with jaundice foal agglutination test in a newborn foal

Aykut Göktürk ÜNER, Güney KAYA, Aydın OLTU

Prevalence and patterns of antimicrobial resistant of fecal Escherichia coli isolates among pigs on eight farrow-to-finish farms and their production environments in Shandong Province, China

Hong-chao ZHOU, Lin LIU, Zhao-fang TIAN, Wen-juan ZHANG

Histopathological changes in uncomplicated sole ulcers in dairy cattle

Ali BELGE, İbrahim AKIN, Recai TUNCA, Erdal ÖZMEN

Evaluation of the possibility of using oligosaccharide-free pea in Japanese quail nutrition

Danuta SZCZERBINSKA, Zofia TARASEWICZ, Piotr GULEWICZ

Effect of cold storage period on the quality of ram cauda epididymal spermatozoa recovered postmortem

Suhail Salam MIR, Farooz Ahmad LONE, Mohammad Zaffarullah KHAN, Asloob Ahmad MALIK, Rafiqul ISLAM, Khursheed Ahmad SOFI