Comparison of different extraction solvents used in GC-MS analysis for detecting volatile odor compounds in heat cow sweat

Abstract: The accurate estrus detection is essential for successful reproduction. Many scientists reported the importance of pheromones for accurate detection of estrus. In addition, the choice of the appropriate device and the appropriate extraction solvent is important for obtaining quickly and more estrus-specific pheromones. In this study, sweat samples were collected in heat cows and volatile organic compounds (VOCs) in the gas chromatography mass spectrometry were evaluated using three different solvents (diethyl ether, dichloromethane, and hexane) for extraction. VOCs with different numbers and different ratios were identified in all three extraction solvents. As the solvent, 9 compounds were detected in diethyl ether, 8 compounds in dichloromethane, and 12 compounds in hexane. Volatile odor compounds common to all three extraction solvents were L-Proline, 1-[O-(1-oxohexyl)–N-[N-[N6-(1-oxohexyl)-N2- [N-(1-oxohexyl)–L-valyl]–L-lysyl]–L-valyl]–L-tyrosyl]-, methyl ester/Tetradecane; 1,2-Benzenedicarboxylic acid, bis (2-methylpropyl) ester; Phenol, 2,2’–methylenebis [6-(1,1-dimethylethyl)–4–methyl-; Palmitin,1,2–di-. According to the results of the analysis, the most volatile compounds were detected when using hexane as the extraction solvent.Key words: GC-MS, estrus, solvent, sweat, volatile compounds

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