Mohamed-Laid HADDI, Leonardo SULAS, Lucia BAILONI, Maroua LIFA, Franco TAGLIAPIETRA, Mirko CATTANI, Matteo GUADAGNIN, Rosella MURESU, Stefano SCHIAVON, Andrea SQUARTINI

Chemical and fermentative characteristics of agricultural byproducts and their mixtures with roughages incubated with rumen fluid from slaughtered dromedaries

In pursuit of novel strategies aimed to promote animal production while exploiting agricultural residues, using the dromedarycamel rumen fluid degradation capabilities combined with a gas production (GP) technique approach, we investigated the in vitrokinetic parameters and some physical and chemical characteristics of three agro-industrial byproducts (AIBP) and their mixtureswith three roughages. Chemical composition, buffering capacity, apparent degradability, pH, and in vitro GP kinetic parameters wereevaluated for citrus pulp, tomato peels, apple pomace, their mixtures (50:50 % DM basis) with milk thistle, crown daisy, and polyphytehay. GP of AIBP ranged from 200 to 240 mL/g DM. Roughages produced significantly less gas whereas mixtures gave intermediateamounts of gas. Gas from AIBP evolved with significantly (P < 0.05) shorter lag time (0.33 h) compared with roughages and mixtures.Mixing roughages with AIBP significantly (P < 0.05) increased the initial and final pH. The buffering capacity significantly increasedwith some mixtures and decreased with some others. In conclusion, ruminal fluid from slaughtered dromedaries, like the conventionalbovine inoculum, fermented the major plant polymers and can be used in in vitro degradability trials. Rapidly fermentable AIPB mixedwith roughages can improve some nutritional aspects, providing dietary fiber and correcting some in vitro kinetic parameters.

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1. FAO. Global food losses and food waste. Extent, causes and prevention. Rome, Italy, 2011.
2. Smith S. Special issue information. Agriculture 2015; 5: 1.
3. Playne MJ, McDonald P. The buffering constituents of herbage and of silage. J Sci Food Agr 1966; 17:264-268.
4. Tagliapietra F, Cattani M, Guadagnin M, Haddi ML, Sulas L, Muresu R, Squartini A, Schiavon S, Bailoni L. Associative effects of poor-quality forages combined with food industry byproducts determined in vitro with an automated gasproduction system. Anim Prod Sci 2015; 55: 1117-1122.
5. Sulas L, Re GA, Bullitta S, Piluzza G. Chemical and productive properties of two Sardinian milk thistle (Silybum marianum L. Gaertn.) populations as sources of nutrients and antioxidants. Genet Resour Crop Ev 2016; 63: 315-326.
6. Haddi ML, Filacorda S, Susmel P. Comparison of different inocula to describe some halophyte shrubs using the gas-test. In: Proceedings of the 8th ASPA Congress, Recent Progress in Animal Production 1. Piacenza, Italy; 1999. pp. 360-362.
7. Valente ME, Borreani G, Caredda S, Cavallarin L, Sulas L. Ensiling forage garland (Chrysanthemum coronarium L.) at two stages of maturity and at different wilting levels. Anim Feed Sci Tech 2005; 108: 181-190.
8. Rainone F. Milk thistle. Am Fam Physician 2005; 7: 1285-1288.
9. Cabiddu A, Addis M, Pinna G, Spada S, Fiori M, Sitzia M, Pirisi A, Piredda G, Molle G. The inclusion of a daisy plant (Chrysanthemum coronarium) in dairy sheep diet. 1. Effect on milk and cheese fatty acid composition with particular reference to C18:2 cis-9, trans-11. Livest Sci 2006; 101: 57-67.
10. Marongiu B, Piras A, Porcedda S, Tuveri E, Laconi S, Deidda D, Maxia A. Chemical and biological comparisons on supercritical extracts of Tanacetum cineraiifolium (Trevir) Sch. Bip. with three related species of chrysanthemums of Sardinia (I). Nat Prod Res 2008; 23: 190-199.
11. Tagliapietra F, Cattani M, Bailoni L, Schiavon S. In vitro rumen fermentation: effect of headspace pressure on the gas production kinetics of corn meal and meadow hay. Anim Feed Sci Tech 2010; 158: 197-201.
12. AOAC. Official Methods of Analysis of the Association of Official Agricultural Chemists, 17th ed. (2nd revision). AOAC International, Gaithersburg, MD, USA, 2003.
13. Mertens DR. Gravimetric determination of amylase treated neutral detergent fiber in feeds with refluxing in beakers or crucibles: collaborative study. J AOAC Int 2002; 85: 1217-1240.
14. Robertson JB, Van Soest PJ. The detergent system of analysis and its application to human foods. In: Tames WPT, Theander O, editors. The Analysis of Dietary Fiber in Food. New York, NY, USA: Marcel Dekker Inc.; 1981. pp. 123-158.
15. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem 1956; 28: 350-356.
16. Tilley GMA, Terry RA. A two stage technique for in vitro digestion of forage crops. J Brit Grassland Soc 1963; 18: 104.
17. Giger-Reverdin S, Duvaux-Ponter C, Sauvant D, Martin O, Prado IN, Muller R. Intrinsic buffering capacity of feedstuffs. Anim Feed Sci Tech 2002; 96: 83-102.
18. Menke KH, Steingass H. Estimation of the energetic feed value obtained from chemical analysis and gas production using rumen fluid. Anim Res Dev 1988; 28: 7-55.
19. Cone JW, van Gelder AH, Visscher GJW, Oudshoorn L. Influence of rumen fluid and substrate concentration on fermentation kinetics with a fully automated time related gas production apparatus. Anim Feed Sci Tech 1996; 61: 113-128.
20. Van Gelder AH. Gas Production Technique (GPT) Instructions. Lelystad, the Netherlands: Animal Sciences Group, Wageningen University, 2005.
21. Ørskov ER, McDonald I. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J Agr Sci 1979; 92: 499- 503.
22. Dhanoa MS. On the analysis of the dracon bag data for low degradability feeds. Grass Forage Sci 1988; 43: 441-444.
23. SAS User’s Guide: Basics. SAS Inst. Inc., Cary, NC, USA, 2005.
24. Besharati M, Taghizadeh A, Janmohammadi H, Moghadam GA. Evaluation of some byproducts using in situ and in vitro gas production technique. Am J Anim Vet Sci 2008; 3: 7-12.
25. Moharrery A. The determination of buffer capacity of some ruminant’s feedstuffs and their cumulative effects on TRM ration. Am J Anim Vet Sci 2007; 2:72-78.
26. Abbeddou S, Hess HD, Rischkowski B, Kreuzer M. Effects of local crop residues and agro-industrial by-products on milk yield and quality of Syrian Awassi ewes. In: Proceedings of Options Méditerranéennes: Série A, Séminaires Méditerranéens; 2011. 99: pp. 365-369.
27. Cribbs JT. Dehydrated citrus pulp alters feedlot performance of crossbred heifers during the receiving period and modulates physiological and acute phase responses and metabolite concentration. MSc, Texas Tech University, Texas, USA, 2012.
28. Villareal M, Cochran RC, Rojas-Bourrillon A, Murillo O, Poore M. Effect of supplementation with pelleted citrus pulp on digestibility and intake in beef cattle fed a tropical grassbased diet (Cynodon nlemfuensis). Anim Feed Sci Tech 2006; 125: 163-167.
29. Abdollahzadeh F, Pirmohammadi R, Fatehi F, Bernousi I. Effects of feeding ensiled mixed tomato and apple pomace on performance of Holstein dairy cows. Slovak J Anim Sci 2010; 43: 31-35.
30. Ahn JH, Jo IH, Lee JS. The use of apple pomace in rice straw based diets in Korean native goats (Capra hircus). Asian Austral J Anim 2002; 15: 1599-1605.
31. Gharechahi J, Zahiri HS, Noghabi KA, Salekdeh GH. In-depth diversity analysis of the bacterial community resident in the camel rumen. Syst Appl Microbiol 2014; 38: 67-76.