Changes in serum biochemical and lipid profile, and fatty acid composition of breast meat of broiler chickens fed supplemental grape seed extract
Changes in serum biochemical and lipid profile, and fatty acid composition of breast meat of broiler chickens fed supplemental grape seed extract
A study was conducted to evaluate the changes in serum biochemical and lipid profile, and fatty acid composition of breastmeat of broiler chickens fed supplemental grape seed extract (GSE). A total of 240 1-day-old male broiler chickens were randomlyallocated to 4 groups, each consisting of 4 replicate pens having 15 birds/replicate pen. One group served as control group fed basaldiets and other groups received 100, 200, and 400 ppm GSE supplemented to the basal diets. Liver weight was greater in broilersfed 200 ppm GSE compared to the other groups (P = 0.004, L = 0.024, C = 0.010). Serum AST and ALT levels decreased (P < 0.001)whereas ALP levels increased with increasing dietary GSE levels (P < 0.001). Serum albumin and total protein levels were lower incontrol in comparison with other groups (P < 0.001). Serum cholesterol, triglyceride, and LDL levels declined (P < 0.001) while HDLlevels increased with increasing supplemental GSE levels (P < 0.001). MUFA levels were greater in breast meat of broiler chickens feddiets supplemented with 100 ppm GSE. On the other hand, PUFA (including n-3 and n-6) were greater in broiler chickens fed controldiets or 400 ppm GSE supplemented diets. In general, essential fatty acids such as α-linolenic acid (n-3 C18:3), arachidonic acid (n-6C20:4), eicosapentaenoic acid (n-3 C20:5), and docosahexaenoic acid (n-3 C22:6) were greater in breast meat of broiler chickens fedsupplemental GSE (100, 200, or 400 ppm) while overall UFA concentration in breast meat remained unaffected. In conclusion, findingsof this study suggest that dietary supplementation of up to 400 ppm GSE in broilers may improve serum biochemical and lipid profile. Itmay also improve the fatty acid composition of breast meat to varying extent despite no effect on overall UFA concentration.
___
- 1. Calabotta DF, Shermer WD. Controlling feed oxidation can be
rewarding. Feedstuffs 1985; 57: 24-33.
- 2. Augustyniak A, Bartosz G, Cipak A, Duburs G, Horáková
LU et al. Natural and synthetic antioxidants: an updated
review. Free Radical Research 2010; 44: 1216-1262. doi:
10.3109/10715762.2010.508495
- 3. Błaszczyk A, Augustyniak A, Skolimowski J. Ethoxyquin: an
antioxidant used in animal feed- a review. International Journal
of Food Science 2013; 2013: 1-12. doi: 10.1155/2013/585931
- 4. Salami SA, Guinguina A, Agboola JO, Omede AA, Agbonlahor
EM et al. In vivo and postmortem effects of feed antioxidants
in livestock: a review of the implications on authorization of
antioxidant feed additives. Animal 2016; 10: 1375-1390. doi:
10.1017/S1751731115002967
- 5. Scalbert A, Williamson G. Dietary intake and bioavailability
of polyphenols. The Journal of Nutrition 2000; 130 (8):
2073S-2085S. doi: 10.1093/jn/130.8.2073S
- 6. Chamorro S, Viveros A, Centeno C, Romero C, Arija I et al.
Effects of dietary grape seed extract on growth performance,
amino acid digestibility and plasma lipids and mineral content
in broiler chicks. Animal 2013; 7: 555-561. doi: 10.1017/
S1751731112001851
- 7. Abu-Hafsa SH, Ibrahim SA. Effect of dietary polyphenol-rich
grape seed on growth performance, antioxidant capacity and
ileal microflora in broiler chicks. Journal of Animal Physiology
and Animal Nutrition 2018; 102: 268-275. doi: 10.1111/
jpn.12688
- 8. Iqbal Z, Ali R, Sultan JI, Kamran Z, Khan SA et al. Impact
of replacing grape polyphenol with vitamin e on growth
performance, relative organs weight and antioxidant status of
broilers. Journal of Animal & Plant Science 2014; 24: 1579-
1583.
- 9. Iqbal Z, Kamran Z, Sultan JI, Ali A, Ahmad S et al. Replacement
effect of vitamin E with grape polyphenols on antioxidant
status, immune, and organs histopathological responses
in broilers from 1- to 35-d age. Journal of Applied Poultry
Research 2015; 24: 127-134. doi: 10.3382/japr/pfv009
- 10. Habibian M, Sadeghi G, Karimi A. Comparative effects
of powder, aqueous and methanolic extracts of purslane
(Portulaca oleracea L.) on growth performance, antioxidant
status, abdominal fat deposition and plasma lipids in broiler
chickens. Animal Production Science 2018; 59: 89-100. doi:
10.1071/AN17352
- 11. Singleton VL, Rossi JA. Colorimetry of total phenolics with
phosphomolybdic-phosphotungstic acid reagents. American
Journal of Enology and Viticulture 1965; 16: 144-158.
- 12. National Research Council. Nutrient Requirements of Poultry.
9th revised ed. Washington, DC: National Academies Press;
1994.
- 13. Karadagoglu Ö, Şahin T, Ölmez M, Özsoy B, Yakan A. Broyler
rasyonlarına farklı düzeylerde ilave edilen üzüm çekirdeği
ekstraktının performans ve bazı karkas özellikleri üzerine
etkisi. In: 2nd International Animal Nutrition Congress;
Antalya, Turkey; 2018. pp. 60-64.
- 14. Sari M, Onk K, Sisman T, Tilki M, Yakan A. Effects of different
fattening systems on technological properties and fatty acid
composition of goose meat. European Poultry Science 2015;
79: doi: 10.1399/eps.2015.79
- 15. Brenes A, Viveros A, Goni I, Centeno C, Sayago-Ayerdy SG et al.
Effect of grape pomace contrate and vitamin E on digestibility
of polyphenols and antioxidant activity in chickens. Poultry
Science 2008; 87: 307-316. doi: 10.3382/ps.2007-00297
- 16. Hajati H, Hassanabada I, Golian AG, Nassiri-Moghaddam H,
Nassiri MR. The effect of grape seed extract and vitamin C feed
supplements carcass characteristics, gut morphology and ileal
microflorain broiler chickens exposed to chronic heat stress.
Iranian Journal of Applied Animal Science 2015; 5: 155-165.
- 17. Brenes A, Viveros A, Goni I, Centeno C, Saura-Calixto F et al.
Effect of grape seed extract on growth performance, protein
and polyphenol digestibilities, and antioxidant activity in
chickens. Spanish Journal of Agricultural Research 2010; 8:
326-333.
- 18. Han N, Htoo Htoo KS, Aung H. Determinants of abnormal liver
function tests in diabetes patients in Myanmar. International
Journal of Diabetes Research 2012; 1 (3): 36-41. doi: 10.5923/j.
diabetes.20120103.02
- 19. Meluzzi A, Primiceri G, Giordani R, Fabris G. Determination
of blood constituents reference values in broilers. Poultry
Science 1992; 71: 337-345. doi: 10.3382/ps.0710337
- 20. Melillo A. Applications of serum protein electrophoresis in
exotic pet medicine. The Veterinary Clinics of North America.
Exotic Animal Practice 2013; 16: 211–225. doi: 10.1016/j.
cvex.2012.11.002
- 21. Farahat MH, Abdallah FM, Ali HA, Hernandez-Santana A.
Effect of dietary supplementation of grape seed extract on
the growth performance, lipid profile, antioxidant status and
immune response of broiler chickens. Animal 2017; 11: 771–
777. doi: 10.1017/S1751731116002251
- 22. Bladé C, Arola L, Salvadó MP. Hypolipidemic effect of
proanthocyanidins and their underlying biochemical and
molecular mechanisms. Molecular. Nutrition & Food Research
2010; 54: 37-59. doi: 10.1002/mnfr.200900476
- 23. Stein JH, Keevil JG, Wiebe DA, Aeschlimann S, Folts JD.
Purple grape juice improves endothelial function and reduces
the susceptibility of LDL cholesterol to oxidation in patients
with coronary artery disease. Circulation 1999; 100: 1050-
1055. doi: 10.1161/01.CIR.100.10.1050
- 24. Tebib K, Besancon P, Rouanet JM. Dietary grape seed tannins
affect lipoproteins, lipoprotein lipases and tissue lipids in rats
fed hypercholesterolemic diets. Journal of Nutrition 1994; 124:
2451-2457. doi: 10.1093/jn/124.12.2451
- 25. Jin LZ, Ho YW, Abdullah N, Jalaludin S. Growth performance,
intestinal microbial populations and serum cholesterol of
broilers diets containing Lactobacillus cultures. Poultry
Science 1998; 77: 1259-1265. doi: 10.1093/ps/77.9.1259
- 26. Roy DM, Schneeman BO. Effect of soy protein, casein and
trypsin inhibitor on cholesterol, bile acids and pancreatic
enzymes in mice. Journal of Nutrition 1981; 111: 878-885. doi:
10.1093/jn/111.5.878
- 27. Chen Q, Wang E, Ma L, Zhai P. Dietary resveratrol increases
the expression of hepatic 7α-hydroxylase and ameliorates
hypercholesterolemia in high-fat fed C57BL/6J mice. Lipids in
Health and Disease 2012; 11: 56. doi: 10.1186/1476-511X-11-
56
- 28. Cuccioloni M, Mozzicafreddo M, Spina M, Tran CN, Falconi
M et al. Epigallocatechin-3-gallate potently inhibits the in vitro
activity of hydroxy-3- methyl-glutaryl-CoA reductase. Journal
of Lipid Research 2011; 52: 897-907. doi: 10.1194/jlr.M011817
- 29. Howes NL, Ahmed Bekhit AE, Burritt DJ, Campbell AW.
Opportunities and implication of pasture-based lamb fattening
to enhance the long-chain fatty acid composition in meat.
Comprehensive Reviews in Food Sciences and Food Safety
2015; 14: 22-36. doi:10.1111/1541-4337.12118