Analysis of Fluids of Hydatid Cysts from Sheep by SDS-PAGE, and Determination of Specific Antigens in Protein Structure by Western Blotting

Protein bands of hydatid fluids of sheep liver were determined by SDS-PAGE and Western blotting, and this was followed by the determination of specific bands of hydatid cysts for sheep and humans. For diagnostic purposes, 30 positive and 20 negative sheep sera, 1 commercially obtained non-infected sheep serum, and 10 posi- tive and 10 negative human sera were used in this experiment. According to our results, the specific protein band for hydatid dis- ease in sheep was determined as 116 kDa, while the specific protein bands for hydatid disease in humans were determined as 68 and 8 kDa. We determined that the diagnosis of hydatid disease in sheep and humans is possible with the use of the purified specific proteins obtained in this study. After putting these research results into practice, there will be no need to import expensive ready-made kits for the diagnosis of hydatid disease in humans.

Analysis of Fluids of Hydatid Cysts from Sheep by SDS-PAGE, and Determination of Specific Antigens in Protein Structure by Western Blotting

Protein bands of hydatid fluids of sheep liver were determined by SDS-PAGE and Western blotting, and this was followed by the determination of specific bands of hydatid cysts for sheep and humans. For diagnostic purposes, 30 positive and 20 negative sheep sera, 1 commercially obtained non-infected sheep serum, and 10 posi- tive and 10 negative human sera were used in this experiment. According to our results, the specific protein band for hydatid dis- ease in sheep was determined as 116 kDa, while the specific protein bands for hydatid disease in humans were determined as 68 and 8 kDa. We determined that the diagnosis of hydatid disease in sheep and humans is possible with the use of the purified specific proteins obtained in this study. After putting these research results into practice, there will be no need to import expensive ready-made kits for the diagnosis of hydatid disease in humans.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Growth Performance and Feed Conversion Efficiency of Siberian Sturgeon Juveniles (Acipenser baeri) Reared in Concrete Raceways

Gülten KÖKSAL, Ferit RAD, Mevlüt KINDIR

A Survey of Hypodermosisin Cattle Slaughtered in Thrace (Trakya) Turkey

Aynur GÜLANBER, Erkut TÜZER, Ayşen GARGILI, Müfit TOPARLAK

Trakya' da mezbahada kesilen sığırlardaHypodermosis

Aynur GÜLANBER, Müfit TOPARLAK, Vedat KELEŞ, Meltem ULUTAŞ, Erkut TÜZER, İlker EFİL, Ayşen GARGILI

Effects of Environmental Temperature and Dietary Ascorbic Acid on the Diurnal Feeding Pattern of Broilers

Hasan Rüştü KUTLU

The Effects of Weaning Age on the Growth, Milk and Milk Fat Characteristics of Brown Swiss Cattle

Mete YANAR, Recep AYDIN

Düşük ve yüksek subklinik mastitis problemiyle karşı karşıya olan İskoçya süt sığırcılık işletmelerinde mastitisten kaynaklanan finansal kayıplar

Cengiz YALÇIN

Epidermal Growth Factor Receptor (EGFR) Immunolocalization in the Male Rat Reproductive Tract During Pre-and Postnatal Periods

Celal KALOĞLU, Hüseyin Eray BULUT, Bilge ONARLIOĞLU

Analysis of Fluids of Hydatid Cysts from Sheep by SDS-PAGE, and Determination of Specific Antigens in Protein Structure by Western Blotting

Ayşe BURGU, Ahmet DOĞANAY, Bahadır GÖNENÇ, H. Oğuz SARIMEHMETOĞLU, Funda KALINBACAK

Escherichia coli 0157:H7 serotipinin izolasyonunda selektif broth ve agar besiyerleri üzerine bir çalışma

Malihe R. NOVEIR, A. Kadir HALKMAN

Cost of Mastitis in Scottish Dairy Herds with Low and High Subclinical Mastitis Problems

Cengiz YALÇIN