Abdullah DOĞAN, Salih OTLU, Özgür ÇELEBİ, Pınar AKSU KILIÇLE, Aliye GÜLMEZ SAĞLAM, Ali Nazmi Can DOĞAN, Neslihan MUTLU

An investigation of antibacterial effects of steroids

In this study, the effects of steroid substances such as corticosterone, progesterone, estrone, beta-sitosterol, and stigmasterol on some bacteria were investigated. In the research, standard strains of the gram-positive bacteria Bacillus subtilis (lab isolate) and Staphylococcus aureus (ATCC 6538) and the gram-negative bacteria Escherichia coli (ATCC 25922), Pasteurella multocida (ATCC 12945), and Pseudomonas aeruginosa (ATCC 9027) were used. A modified broth dilution method was applied for determining the minimal inhibitory concentration (MIC) of the steroids. Mueller Hinton broth (Becton Dickinson, 211443) was used to prepare dilutions and as a medium. At the end of the study, the MIC of corticosterone for P. multocida and the MIC of beta-sitosterol for S. aureus were both determined as 32 μg/mL. However, other steroids showed no effects on the bacteria. These steroids did not appear to have widespread antibacterial properties.

Keywords:

Corticosterone, progesterone, estrone, beta-sitosterol, stigmasterol, antibacterial,

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