An immunohistochemical study on the distribution of endocrine cells in the digestive tract of gray goose (Anser anser)

The objective of this study was to investigate the morphology and the distribution of 5-hydroxytryptamine (5-HT), somatostatin (SS), gastrin (Gas), glucagon (Glu), and substance P immunoreactive (IR) cells in the digestive tract of gray goose by the immunohistochemical streptavidin-peroxidase method. The samples were taken from 10 healthy adult gray geese. The results showed that 5 kinds of IR cells were mainly distributed between the mucous epithelium and intestinal gland. The number of 5-HT-IR cells was highest in the rectum and duodenum, but none were observed in the pylorus. SS-IR cells appeared in great numbers in the pylorus, duodenum, and cecum; however, they were not found in esophagus. Gas-IR cells were mainly distributed in the glandular stomach and jejunum. Glu-IR cells appeared in small numbers in the glandular stomach, duodenum, and jejunum, but were not detected in other tissues. Substance P-IR cells were located in the jejunum, cecum, and rectum. Analysis of the present study showed that the distribution and morphological features of these 5 different endocrine cells were related to the feeding habits and metabolism in the digestive tract of the gray goose.

An immunohistochemical study on the distribution of endocrine cells in the digestive tract of gray goose (Anser anser)

The objective of this study was to investigate the morphology and the distribution of 5-hydroxytryptamine (5-HT), somatostatin (SS), gastrin (Gas), glucagon (Glu), and substance P immunoreactive (IR) cells in the digestive tract of gray goose by the immunohistochemical streptavidin-peroxidase method. The samples were taken from 10 healthy adult gray geese. The results showed that 5 kinds of IR cells were mainly distributed between the mucous epithelium and intestinal gland. The number of 5-HT-IR cells was highest in the rectum and duodenum, but none were observed in the pylorus. SS-IR cells appeared in great numbers in the pylorus, duodenum, and cecum; however, they were not found in esophagus. Gas-IR cells were mainly distributed in the glandular stomach and jejunum. Glu-IR cells appeared in small numbers in the glandular stomach, duodenum, and jejunum, but were not detected in other tissues. Substance P-IR cells were located in the jejunum, cecum, and rectum. Analysis of the present study showed that the distribution and morphological features of these 5 different endocrine cells were related to the feeding habits and metabolism in the digestive tract of the gray goose.

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  • Larsson, L.I., Sundler, F., Hakanson, R., Rehfi eld, J.F., Stadil, F.: Distribution and properties of gastrin cells in the gastrointestinal tract of chicken. Cell Tissue Res., 1974; 154: 409-422.
  • Deng, Z.P., Zhou, Z.X., Yamada, J.: Immunohistochemical localization of endocrine cells in the gastrointestinal tract of goose. Acta Anatomica Sinica, 1996; 27: 314-317. 9. El-Salhy, M., Wilander, E., Lundquist, M.: Comparative studies of serotonin-like immunoreactive cell in the digestive tract of vertebrates. Biomed. Res., 1985; 6:371-375.
  • Tang, X.W., Deng, Y.S.: Distribution of 5-hydroxytryptamine immunostaining cells in digestive tracts of diff erent kind vertebrates. Zoological Research, 1991; 12: 293-297.
  • Liu, B., Zeng, S.J., Lin, Y.D., Zhang, C.L., Li, F.L.: Location of endocrine cells in the gastrointestinal tract of White Ibis. Zoological Research, 2004; 25: 442-446.
  • Li, S.L., Leng, C., Zhao, W.Y.: Observation of 5-HT immunoreactive cells in digestive tract of Gallus domestius. Chinese Journal of Anatomy, 2005; 28: 292-294. 13. Gülmez,  N.,  Kocamış,  H.,  Immunohistochemical distribution of cells containing insulin, glucagon and somatostatin in the goose (Anser anser) pancreas. Turk. J. Vet. Anim. Sci., 2004; 28: 403-407. S.,  Nazlı,  M.:
  • Wang, X.L., Fang, H.L., Zhou, F., Qin, J., Nong, C.T.: Immunohistochemical location and morphological characteristics of gastrointestinal endocrine cell of Gorsachius magnifi cus. Chinese Journal of Veterinary Science and Technology, 2005; 35: 570-573.
  • Huang, X.G., Wu, X.B.: Immunohistochemical localization of endocrine cells in the digestive tract of Sphenomorphus indicus. Chinese Journal of Histochemistry and Cytochemistry, 2006; 15: 233-238.
  • Shi, Z.Y., Chen, X.W., Gu, Y.F.: Histological studies on gastrointestinal tract of Paralichthys olivaceus and endocrine cells distribution within it. Journal of Fishery Sciences of China, 2006; 13: 851-854.
  • Raybould, H.E., Glatzle, J., Robin, C., Meyer, J.H., Phan, T., Wong, H., Sternini, C.: Expression of 5-HT3 receptor by extrinsic duodenal aff erent to intestinal inhibition of gastric emptying. Am. J. Physiol., 2003; 284: 367-372.
  • Cooke, H.J.: Neurotransmitters in neuronal refl exes regulating intestinal secretion. Ann. NY Acad. Sci., 2000; 915: 77-80.
  • Yang, G.B, Lackner, A.A.: Proximity between 5-HT secreting enteroendocrine cells and lymphocytes in the gut mucosa of rhesus macaques (Macaca mulatta) is suggestive of a role for enterochromaffin cell 5-HT in mucosal immunity. J. Neuroimmunol., 2004; 146: 46-49.
  • Jiang, Y.L., Feng, J., Wang, L.M.: Zoology. 1st ed., Higher Education Publishing, Beijing. 2006; 301.
  • Zhang, L., Liu, X.J., Dong, W., Zhang, Y.J., Yang, J.: Expression of 5-HT in the gastrointestinal tract of Zi goose in diff erent ages. Chinese Journal of Anatomy, 2009; 32: 626-628.
  • Liu, X.J., Yang, J., Zhang, L., Zhang, L.: Study on the endocrine cells in the digestive tract of Arctic fox (Alopex lagopus) using immunohistochemical methods. Acta Th eriologica Sinica, 2009; 29: 338-342.
  • Li, S.L., Yu, Y.Z., Gao, X., Liu, C., Zhao, W.G., Sun, Y.G.: Immunohistochemical studies on endocrine cells in the digestive tract of Takydromus wolteri. Chinese Journal of Zoology, 2008; 43: 43-50.
  • Li, S.L., Han, L.X., Zhen, L.J., Yu, H.B., Yu, Y.Z., Qu, L.D.: Immunohistochemical localization of 5-HT cells in gastrointestinal tract of HBK-SPF ducks of 40 weeks old. Chinese Journal of Comparative Medicine, 2008; 18: 27-36.
  • Gulmez, N., Nazli, M., Aslan, S., Liman, N.: Immunolocalisation of serotonin, gastrin, somatostatin and glucagon in entero- endocrine cells of the goose (Anser anser). ACTA Vet Hung. 2003; 51: 439-449.
  • Morescalchi, A.M., Gaccioli, M., Faraldi, G., Tagliafi erro, G.: Th e gastro-enteric-pancreatic neuroendocrine system in two reptilian species: Chalcides chalcides and Zoonosaurus madascariensis (Sauridae). Eur. J. Histochem., 1997; 41: 29-34.
  • Nilsson, A.H.: Th e gut as the largest endocrine organ in the body. Ann. Oncol., 2001; 12: 63-68.
  • Chen, L., Zhang, H.H.: Immunohistochemical study of endocrine cell in the digestive track of wolves and domestic dogs. Acta Anatomica Sinica, 2008; 39: 413-419.
  • Yang, Q., Chen, S.H., Zhao, R.X., Chen, J.: Ontogeny of somatostatin positive-cells and immunocytes in the porcine duodenum. Chinese Journal of Animal and Veterinary Sciences, 2004; 35: 60-63.
  • Goto, T., Yamaza, T., Kido, M.A., Tanaka, T.: Light- and electron- microscopic study of the 0,20 thought d it isdistribution of axons containing substance p and the localization of neurokinin-1 receptor in bone. Cell Tissue Res., 1998; 293: 87-93.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
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