Farzad ASADI, Malihe POURKABIR, Robin MACLAREN, Ali SHAHRIARI

Alterations to Lipid Parameters in Response to Fig Tree (Ficus carica) Leaf Extract in Chicken Liver Slices

Lipid storage, particularly in the abdominal cavity, is a major concern in poultry breeding because it affects the net meat yield. The major contributors to lipid storage in the abdominal cavity are hepatic fatty acid synthesis and VLDL secretion. These factors are strongly correlated with the body fatness in the chicken. The aim of our study was to determine if a traditional food supplement, the extract from fig tree leaves (fig tree leaf extract (FTE)), can be used to decrease hepatic triglyceride (TG) content and secretion of TG and cholesterol (TC) from the liver. Livers from 8-week-old roosters (n = 24) with high abdominal fat pad ratios were extracted, sliced, and cultured with increasing concentrations of FTE, insulin and both of them. While insulin significantly increased TG secretion (0.190 ± 0.013 mmol/l), TG content (0.523 ± 0.093 mmol/l) and TC secretion (1.727 ± 0.412 mmol/l) above the basal levels (P < 0.001), when FTE was added these effects were drastically reduced to the basal levels in a concentration-dependent manner (P < 0.001). Furthermore, we showed that in response to 1.7, 2.5 and 3.3 µl of FTE/ml of media hepatic TG content and TG secretion values from the liver significantly decreased in a concentration-dependent manner when compared to the control (P < 0.001). Our results suggest that Ficus carica leaf extract could be a beneficial supplement to modulate TG and TC secretion from the poultry liver.

Anahtar Kelimeler:

Fig tree leaf, chicken, lipid, liver

Alterations to Lipid Parameters in Response to Fig Tree (Ficus carica) Leaf Extract in Chicken Liver Slices

Keywords:

Fig tree leaf, chicken, lipid, liver,

PDF

___

Hemmatzadeh, F., Fatemi, A., Amini, F.: Therapeutic effects of fig tree latex on bovine papillomatosis. J. Vet. Med. B Infect. Dis. Vet. Public Health, 2003; 50: 473-476.
Torres, M.D., Dominguez, E., Romero, A., Campillo, J.E., Perez, C.: Hypoglycemic and hypolipidemic activity of an aqueous extract from Ficus carica in streptozotocin diabetic rats. Diabetologia, 1993; 36 (Suppl. 1): A-181.
Pullen, D.L., Liesman, J.S., Emery, R.S.: A species comparison of liver slice synthesis and secretion of triacylglycerol from nonesterified fatty acids in media. J. Anim. Sci., 1990; 68: 1395- 1399.
Hara, A., Radin, N.S.: Lipid extraction of tissues with a low toxicity solvent. Anal. Biochem., 1978; 90: 420-26.
Neri, B.P., Fring, C.S.: Improved methods for determination of triglyceride in serum. Clin. Chem., 1973; 19: 1201-1203.
Loeffler, H.H., McDougald, C.H.: Estimation of Cholesterol in Serum by means of Improved Techniques. In: Clinical Chemistry Principles and Procedures. J.S. Annino and R.W. Giese editors. Little Brown and Company Boston Press, 1963; 279-282.
Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 1976; 72: 248-254.
Whitehead, C.C., Griffin, H.D.: Plasma lipoprotein concentration as an indicator of fatness in broilers: effect of age and diet. Br. Poult. Sci., 1982; 23: 299-305.
Tarlow, D.M., Watkins, P.A., Reed, R.E., Miller, R.S., Zwergel, E.E., Lane, M.D.: Lipogenesis and the synthesis and secretion of very-low-density lipoprotein by avian liver cells in nonproliferating monolayer culture. Hormonal effects. J. Cell Biol., 1977; 73: 332-353.
Siuta-Mangano, P., Janero, D.R., Lane, M.D.: Association and assembly of triglyceride and phospholipid with glycosylated and unglycosylated apoproteins of very-low-density lipoprotein in the intact liver cell. J. Biol. Chem., 1982; 257: 11463-11467.