Effects of organic and inorganic nitrogen sources on in vitro degradability of citrus byproduct and milk thistle
In order to evaluate rumen microbial activity, the effects of two nitrogen sources (organic and inorganic) were analyzed in vitro by inoculating dairy cow rumen fluid and using citrus byproduct (CBP) and milk thistle (Silybum marianum, MT) as energy source substrates. The experimental design was 2 × 3 where we used three different nitrogen sources (N1: no nitrogen added, N: ammonium bicarbonate, N3: sodium glutamate) with a basic concentration of nitrogen (100 mg/1 L). Culture pH, dry matter degradability %, ammonia-N (NH33_{3}-N), total volatile fatty acids (TVFAs), individual volatile fatty acid proportions, and acetate propionate ratio (A:P) were measured after 24 h of fermentation. Culture pH values were in the optimal range with CBP and MT in all media. Supplementing nitrogen sources had similar tendencies to increase dry matter degradability (P < 0.01) and NH33_{3}-N production in N2 and N3 with MT and CBP. Glutamate significantly (P < 0.01) increased TVFAs, which were twice as high than in N1 and N2 with MT. These results show that N supplementation had minor effects on in vitro rumen microbiota activity compared to N1; however, it could be proposed to improve microbial biomass production in mixed rations of agricultural byproducts with local forages.
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- Palomino O. Assessment Report on
Silybum marianum
(L.)
Gaertn., Fructus. London, UK: European Medicines Agency;
2015.
- Quézel P, Santa S. Nouvelle flore de l’Algérie et des régions
désertiques méridionale, Tome II. Paris, France: Centre
National de la Recherche Scientifique; 1963 (in French).
- Sindel BM. A review of the ecology and control of thistles in
Australia. Weed Res 1991; 31: 189-201.
- Martini V. Cinetica di degradazione microbica di residui agro-
industriali mediante tecnica GP (gas production). Thesis,
University of Padua, Padua, Italy, 2009 (in Italian).
- Strobel HJ, Russell JB. Effect of pH and energy spilling on
bacterial protein synthesis by carbohydrate-limited cultures of
mixed rumen bacteria. J Dairy Sci 1986; 69: 2941-2947.
- Miron J, Yosef E, Ben-Ghedalia D. Composition and in vitro
digestibility of monosaccharide constituents of selected
byproduct feeds. J Agr Food Chem 2001; 49: 2322-2326.
- Bampidis VA, Robinson PH. Citrus by-products as ruminant
feeds: a review. Anim Feed Sci Tech 2006; 128: 175-217.
- Wing JM, Van Horn HH, Sklare SD, Harris B Jr. Effects of
citrus molasses, distillers solubles and molasses on rumen
parameters and lactation. J Dairy Sci 1988; 71: 414-420.
- Getachew G, Blümmel M, Makkar HPS, Becker K. In vitro gas
measuring techniques for assessment of nutritional quality of
feeds: a review. Anim Feed Sci Tech 1998; 72: 261-281.
- Leng RA, Nolan JV. Nitrogen metabolism in the rumen. J Dairy
Sci 1984; 67: 1072-1089.
- Dryhurst N, Wood CD. The effect of nitrogen source and
concentration on in vitro gas production using rumen micro-
organisms. Anim Feed Sci Tech 1998; 71: 131-143.
- AOAC. Official Methods of Analysis. 17th ed. Gaithersburg,
MD, USA: Association of Official Agricultural Chemists; 2003.
- Mertens DR. Gravimetric determination of amylase-treated
neutral detergent fiber in feeds with refluxing in beakers or
crucibles: collaborative study. J AOAC Int 2002; 85: 1217-1240.
- Robertson JB. The detergent system of fiber analysis. In: Spiller
GA, editor. Topics in Dietary Fiber Research. New York, NY,
USA: Plenum Press; 1978. pp. 18-32.
- Weatherburn MW. Phenol-hypochlorite reaction for
determination of ammonia. Anal Chem 1967; 39: 971-974.
- Eun JS, Beauchemin KA. Enhancing in vitro degradation of
alfalfa hay and corn silage using feed enzymes. J Dairy Sci
2007; 90: 2839-2851.
- SAS Inc. SAS Statistic Software. Cary, NC, USA: SAS; 2007.
- Peixoto ELT, Morenz MJF, Da Fonseca CEM, Dos Santos Moura
E, De Lima KR, Lopes FCF, Da Silva Cabral L. Citrus pulp
in lamb diets: intake, digestibility, and ruminal parameters.
Semina Ciências Agrárias Londrina 2015; 36: 3421-3430.
- NRC. Nutrient Requirements of Dairy Cattle: 7th Revised
Edition. Washington, DC, USA: National Academic Press;
2001.
- Tagliapietra F, Cattani M, Guadagnin M, Haddi ML, Sulas
L, Muresu R, Squartini A, Schiavon S, Bailoni L. Associative
effects of poor-quality forages combined with food industry
byproducts determined in vitro with an automated gas-
production system. Anim Prod Sci 2015; 55: 1117-1122.
- Thivend P, Fonty G, Jouany JP, Durand M, Gouet P. Le
fermenteur rumen. Reproduction, Nutrition et Développement
1985; 25: 729-753 (in French).
- Hungate RE. The Rumen and its Microbes. New York, NY,
USA: Academic Press; 1966.
- Blümmel M, Lebzien P. Predicting ruminal microbial
efficiencies of dairy rations by in vitro techniques. Livestock
Prod Sci 2001; 68: 107-117.
- Grings EE, Blümmel M, Sudekum KH. Methodological
considerations in using gas production techniques for
estimating ruminal microbial efficiencies for silage-based
diets. Anim Feed Sci Tech 2005; 123-124: 527-545.
- Blümmel M, Aiple KP, Steingaβ H, Becker K. A note on
the stoichiometrical relationship of short chain fatty acid
production and gas formation
in vitro
in feedstuffs of widely
differing quality. J Anim Physiol An N 1999; 81: 157-167.
- Blümmel M, Orskov ER. Comparison of in vitro gas production
and nylon bag degradability of roughages in predicting feed
intake in cattle. Anim Feed Sci Tech 1993; 40: 109-119.
- Maeng WJ, Van Nevel CJ, Baldwin RL, Morris JG. Rumen
microbial growth rates and yields: effect of amino acids and
protein. J Dairy Sci 1976; 59: 86-79.
- Russell JB, Cook GM. Energetics of bacterial growth: balance
of anabolic and catabolic reactions. Microbiol Rev 1995; 59:
48-62.
- Tamminga S, Hof G. Feeding system for dairy cows. In:
Theodorou MK, France J, editors. Feeding Systems and Feed
Evaluation Models. Wallingford, UK: CAB International; 2000.
pp. 109-123.
- Kajikawa H, Mitsumori M, Ohmomo S. Stimulatory and
inhibitory effects of protein amino acids on growth rate and
efficiency of mixed ruminal bacteria. J Dairy Sci 2002; 58:
2015-2022.