The effects of formaldehyde intoxication on the inducible nitric oxide synthase expression and nitric oxide level in the liver tissue of rats

To investigate inductive activity of formaldehyde (FA) on the expression of inducible nitric oxide synthase (iNOS) and level of nitric oxide (NO) in the liver of rats. Materials and methods: Sixteen male Wistar albino rats of the same age (3 months old) were divided into 2 experimental groups, with 8 rats as the control and 8 rats formaldehyde-treated (FAt). The FAt group received intraperitoneal injection of 10 mg/kg FA for 10 days. Activity of iNOS was shown with immunohistochemical stain kits, and the concentration of NO level was measured by spectrophotometric method in the liver tissue. Results: The FAt group had increased iNOS expression and NO levels in liver tissue compared to controls. Conclusion: FA increased iNOS expression and NO production in liver tissue of rats.

The effects of formaldehyde intoxication on the inducible nitric oxide synthase expression and nitric oxide level in the liver tissue of rats

To investigate inductive activity of formaldehyde (FA) on the expression of inducible nitric oxide synthase (iNOS) and level of nitric oxide (NO) in the liver of rats. Materials and methods: Sixteen male Wistar albino rats of the same age (3 months old) were divided into 2 experimental groups, with 8 rats as the control and 8 rats formaldehyde-treated (FAt). The FAt group received intraperitoneal injection of 10 mg/kg FA for 10 days. Activity of iNOS was shown with immunohistochemical stain kits, and the concentration of NO level was measured by spectrophotometric method in the liver tissue. Results: The FAt group had increased iNOS expression and NO levels in liver tissue compared to controls. Conclusion: FA increased iNOS expression and NO production in liver tissue of rats.

___

  • Cheng G, Shi Y, Sturla SJ, Jalas JR, McIntee EJ, Villalta PW et al. Reactions of formaldehyde plus acetaldehyde with deoxyguanosine and DNA: formation of cyclic deoxyguanosine adducts and formaldehyde cross-links. Chem Res Toxicol 2003; 16: 145–52.
  • Metz B, Kersten GF, Hoogerhout P, Brugghe HF, Timmermans HA, De Jong A et al. Identification of formaldehyde-induced modifications in proteins: reactions with model peptides. J Biol Chem 2004; 279: 6235–43.
  • Conaway CC, Whysner J, Verna LK, Williams GM. Formaldehyde mechanistic data and risk assessment: endogenous protection from DNA adduct formation. Pharmacol Ther 1996; 71: 29–55.
  • Trezl L, Hullan L, Szarvas T, Csiba A, Szende B. Determination of endogenous formaldehyde in plants (fruits) bound to L-arginine and its relation to the folate cycle, photosynthesis and apoptosis. Acta Biol Hung 1998; 49: 253–63.
  • Smith AE. Formaldehyde. Occup Med 1992; 42: 83–8.
  • Soffritti M, Maltoni C, Maffei F, Biagi R. Formaldehyde: an experimental multipotential carcinogen. Toxicol Ind Health 1989; 5: 699–30.
  • Kilburn KH. Neurobehavioural impairment and seizures from formaldehyde. Arch Env Health 1994; 49: 37–44.
  • Chowdhury AR, Gautam AK, Patel KG, Trivedi HS. Steroidogenic inhibition in testicular tissue of formaldehyde exposed rats. Indian J Physiol Pharmacol. 1992; 36: 162–8.
  • Kaplan İ, Aydın Y, Bilen Y, Genç F, Keleş MS, Eroğlu A. The evaluation of plasma arginine, arginase, and nitric oxide levels in patients with esophageal cancer. Turk J Med Sci 2012; 42: 403–9.
  • Akar İ, Rahman A, Çolak MC, Üstündağ B, Özercan İH, Uysal A. Immunohistochemical evaluation of the effects of nebivolol on intimal hyperplasia following endothelial injury. Turk J Med Sci 2011; 41: 53–63.
  • Maffei CM, Mirels LF, Sobel RA, Clemons KV, Stevens DA. Cytokine and inducible nitric oxide synthase mRNA expression during experimental murine cryptococcal meningoencephalitis. Infect Immun 2004; 72: 2338–49.
  • Wen J, Rirbeiro R, Zhang Y. Specific PKC isoforms regulate LPS stimulated iNOS induction in murine microglial cells. J Neuroinflammation 2011; 8: 38–40.
  • Cortas NK, Wakid NW. Determination of inorganic nitrate in serum and urine by a kinetic cadmium reduction method. Clin Chem 1990; 36: 1440–3.
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 183: 265–75.
  • McNaughton L, Puttagunta L, Martinez-Cuesta MA, Kneteman N, Mayers I, Moqbel R et al. Distribution of nitric oxide synthase in normal and cirrhotic human liver. Proc Natl Acad Sci U S A 2002; 99: 1716–7.
  • Gurel A, Coskun O, Armutcu F, Kanter M, Ozen OA. Vitamin E against oxidative damage caused by formaldehyde in frontal cortex and hippocampus: biochemical and histological studies. J Chem Neuroanat 2005; 29: 173–8.
  • Gulec M, Gurel A, Armutcu F. Vitamin E protects against oxidative damage caused by formaldehyde in the liver and plasma of rats. Mol Cell Biochem 2006; 290: 61–7.
  • Franklin P, Dingle P, Stick S. Raised exhaled nitric oxide in healthy children is associated with domestic formaldehyde levels. Am J Respir Crit Care Med 2000; 161: 1757–9.
  • Speit G. The implausibility of systemic genotoxic effects measured by the comet assay in rats exposed to formaldehyde. J Proteome Res 2006; 5: 2523–4.
  • Förstermann U, Kleinert H. Nitric oxide synthase: expression and expressional control of the three isoforms. Naunyn Schmiedebergs Arch Pharmacol 1995; 352: 351–64.
  • Horie A, Nagai K, Ohkura S, Ohama T, Komatsu H, Sato K. Proinflammatory cytokines suppress the expression level of protease-activated receptor-2 through the induction of iNOS in rat colon. J Vet Med Sci 2009; 71: 1609–15.
  • Yabuuchi K, Maruta E, Minami M, Satoh M. Induction of interleukin-1 beta mRNA in the hypothalamus following subcutaneous injections of formalin into the rat hind paws. Neurosci Lett 1996; 207: 109–12.
  • Persoz C, Achard S, Leleu C, Momas I, Seta N. An in vitro model to evaluate the inflammatory response after gaseous formaldehyde exposure of lung epithelial cells. Toxicol Lett 2010; 195: 99–105.
  • Cikmaz S, Kutoglu T, Kanter M, Mesut R. Effect of formaldehyde inhalation on rat livers: a light and electron microscopic study. Toxicol Ind Health 2010; 26: 113–9.
  • Strubelt O, Younes M, Pentz R, Kühnel W. Mechanistic study on formaldehyde-induced hepatotoxicity. J Toxicol Environ Health 1989; 27: 351–66.
  • Ku RH, Billings RE. Relationships between formaldehyde metabolism and toxicity and glutathione concentrations in isolated rat hepatocytes. Chem Biol Interact 1984; 51: 25–36.
  • Dumont M, D’Hont C, Moreau A, Mbape H, Feldmann G, Erlinger S. Retrograde injections of formaldehyde into the biliary tree induce alterations of biliary epithelial function in rats. Hepatology 1996; 24: 1217–23.
  • Lino-Dos-Santos-Franco A, Domingos HV, de Oliveira AP, Breithaupt-Faloppa AC, Peron JP, Bolonheis S et al. Differential effects of formaldehyde exposure on the cell influx and vascular permeability in a rat model of allergic lung inflammation. Toxicol Lett 2010; 197: 211–8.  
  • Kamata E, Nakadate M, Uchida O, Ogawa Y, Suzuki S, Kaneko T et al. Results of a 28-month chronic inhalation toxicity study of formaldehyde in male Fisher-344 rats. J Toxicol Sci 1997; 22: 239–54.
  • Beall JR, Ulsamer AG. Formaldehyde and hepatotoxicity: a review. J Toxicol Environ Health 1984; 14: 11–21.
Turkish Journal of Medical Sciences-Cover
  • ISSN: 1300-0144
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

The effects of antioxidants on testicular apoptosis and oxidative stress produced by cell phones

Akif KOÇ, Doğan ÜNAL, Reyhan BAYRAK, Ömer Faruk KARATAŞ, Ersin ÇİMENTEPE, Mehmet Erol YILDIRIM, Murat AYDIN, Ömer BAYRAK

Preliminary data from a surveillance study on surgical site infections and assessment of risk factors in a university hospital

Mustafa NAMIDURU, Rıza ÇAM, Ayşe Özlem METE, Vuslat BOŞNAK, İlkay KARAOĞLAN

Amount of lateral cortex loss in the femur while inserting a DHS-plate

Mahmut Nedim AYTEKİN, Ali Fırat ESMER, Necdet SAĞLAM, Fuat AKPINAR, Fatih KÜÇÜKDURMAZ

A small outbreak of tularemia in a rural area

İbak GÖNEN

The effects of an adaptive directional BEAM microphone on the mismatch negativity responses of cochlear implant users in noise

Ferda AKDAŞ, Asuman ERDOĞAN

Molecular characterization of Acanthamoeba isolated from Kayseri well water

Ülfet ÇETİNKAYA, Süheyla DOĞAN, Çağrı SAKALAR, Süleyman YAZAR, Salih KUK

Effects of obstructive sleep apnea syndrome on temperament and character

Ömer ÖZBULUT, Apdullah AYÇİÇEK, Ahmet Hakkı AŞIK, Mehmet ÜNLÜ, Fatma FİDAN

Intermittent pneumatic compression pump in upper extremity impairments of breast cancer-related lymphedema

Hülya UZKESER, Saliha KARATAY

Investigations of ALS1 and HWP1 genes in clinical isolates of Candida albicans

Burçin ÖZER, Nizami DURAN, Mustafa Altay ATALAY, Yüsüf ÖNLEN, Ayşe Nedret KOÇ, Ömer EVİRGEN, Melek İNCİ, Süleyman DURMAZ, Vicdan MOTOR KÖKSALDI, Çetin KILINÇ

Investigation of the protective effects of L-carnitine and L-arginine on cardiovascular changes induced by ACTH and dexamethasone in rabbits

Metehan UZUN, Birkan TOPÇU