Akif KOÇ, Doğan ÜNAL, Reyhan BAYRAK, Ömer Faruk KARATAŞ, Ersin ÇİMENTEPE, Mehmet Erol YILDIRIM, Murat AYDIN, Ömer BAYRAK

The effects of antioxidants on testicular apoptosis and oxidative stress produced by cell phones

The aim of this study was to determine the probable effects of cellular phone usage on apoptosis and oxidative/antioxidative balance in testicular tissue, and the probable protective role of vitamin C and E on these processes. Materials and methods: Forty-two male rats were divided into 6 groups: control, antioxidant, calling, stand-by, calling plus antioxidant, and stand-by plus antioxidant. Oxidant and antioxidant parameters and apoptotic indexes were studied. Two identical cell phones were used in the calling and stand-by groups. Results: Cell phone usage significantly increased apoptosis in call, but not in the stand-by position, in testicular tissue. Apoptosis produced in the calling mode was significantly more excessive than in the stand-by mode. Oxidative stress was found as high to some extent in both calling and stand-by positions. However, no ameliorating effect of antioxidants could be demonstrated on either apoptosis or oxidative stress. Conclusion: The effects of cell phones on testicular apoptosis and oxidative stress in the calling and stand-by positions and the effects of antioxidants on both injuries have been investigated for the first time. In addition, increased apoptosis by cell phones has been shown for the first time in this report.

Anahtar Kelimeler:

Key words: Cellular phone, apoptosis, oxidative stress, vitamin C, vitamin E

The effects of antioxidants on testicular apoptosis and oxidative stress produced by cell phones

Keywords:

Key words: Cellular phone, apoptosis, oxidative stress, vitamin C, vitamin E,

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GSMA Head Office. Five rules for creating the perfect mobile storm. Available at http://www.gsma.com/membership/wp- content/uploads/2012/03/Velti_white_paper_Perfect_Mobile_ Storm.pdf. 2.
Oral B, Guney M, Ozguner F, Karahan N, Mungan T, Comlekci
S et al. Endometrial apoptosis induced by a 900-MHz mobile
phone: preventive effects of vitamins E and C. Adv Ther 2006; 23: 957–73. 3. Dasdag S, Akdag MZ, Ulukaya E, Uzunlar AK, Ocak AR. Effect of mobile phone exposure on apoptotic glial cells and status of oxidative stress in rat brain. Electromagn Biol Med 2009; 28: 342–54.
Khaki AA, Tubbs RS, Shoja MM, Rad JS, Khaki A, Farahani RM et al. The effects of an electromagnetic field on the boundary tissue of the seminiferous tubules of the rat: a light and transmission electron microscope study. Folia Morphol (Warsz) 2006; 65: 188–94.
Lee JS, Ahn SS, Jung KC, Kim YW, Lee SK. Effects of 60 Hz electromagnetic field exposure on testicular germ cell apoptosis in mice. Asian J Androl 2004; 6: 29–34. 6. Saygin M, Caliskan S, Karahan N, Koyu A, Gumral N, Uguz A. Testicular apoptosis and histopathological changes induced by a 2.45 GHz electromagnetic field. Toxicol Ind Health 2011; 27: 455–63.
Sarvalues.com, Inc. SAR Values. Available at http://www. sarvalues.com/eu-complete.html. Accessed 16 April 2012. 8. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34: 497–500. 9. Aebi H. Catalase in vitro. Methods Enzymol 1984; 105: 121–6.
Mihara M, Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 1978; 86: 271–8.
Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70: 158–69.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265–75.
Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol 2007; 35: 495–516.
Özdemir B, Kaya A, Söğüt Ö, Kaya H, Gökdemir MT, Celebiş O. Oxidative stress status of individuals involved in traffic accidents. Turk J Med Sci 2012; 42: 507–14.
Zhao TY, Zou SP, Knapp PE. Exposure to cell phone radiation up-regulates apoptosis genes in primary cultures of neurons and astrocytes. Neurosci Lett 2007; 412: 34–8.
Dasdag S, Akdag MZ, Ulukaya E, Uzunlar AK, Yegin D. Mobile phone exposure does not induce apoptosis on spermatogenesis in rats. Arch Med Res 2008; 39: 40–4.
Yilmaz F, Dasdag S, Akdag MZ, Kilinc N. Whole-body exposure of radiation emitted from 900 MHz mobile phones does not seem to affect the levels of anti-apoptotic bcl-2 protein. Electromagn Biol Med 2008; 27: 65–72.
Malaric K, Bartolic J, Malaric R. Measurement of GSM phone emission. IEEE Instrumentation and Measurement Technology Conference Proceedings 2004; 1: 263–6.
Dasdag S, Zulkuf Akdag M, Aksen F, Yilmaz F, Bashan M, Mutlu Dasdag M et al. Whole body exposure of rats to microwaves emitted from a cell phone does not affect the testes. Bioelectromagnetics 2003; 24: 182–8.
Gutteridge JM. Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem 1995; 41: 1819–28.
Mailankot M, Kunnath AP, Jayalekshmi H, Koduru B, Valsalan R. Radio frequency electromagnetic radiation (RF-EMR) from GSM (0.9/1.8GHz) mobile phones induces oxidative stress and reduces sperm motility in rats. Clinics (Sao Paulo) 2009; 64: 561–5.
Ilhan A, Gurel A, Armutcu F, Kamisli S, Iraz M, Akyol O et al. Ginkgo biloba prevents mobile phone-induced oxidative stress in rat brain. Clin Chim Acta 2004; 340: 153–62.
Meral I, Mert H, Mert N, Deger Y, Yoruk I, Yetkin A et al. Effects of 900-MHz electromagnetic field emitted from cellular phone on brain oxidative stress and some vitamin levels of guinea pigs. Brain Res 2007; 1169: 120–4.
Ozguner F, Bardak Y, Comlekci S. Protective effects of melatonin and caffeic acid phenethyl ester against retinal oxidative stress in long-term use of mobile phone: a comparative study. Mol Cell Biochem 2006; 282: 83–8.