Bünyamin MUSLU, Hüseyin SERT, Muhammet GÖZDEMİR, Rüveyda İrem DEMİRCİOĞLU, Burhanettin USTA

Taurine is protective against oxidative stress during cold ischemia in the rat kidney

Recent studies in rats have shown that taurine can prevent oxidative changes induced in renal tissue by ischemia and reperfusion. The aim of the present study was to investigate whether taurine can prevent oxidative changes that occur in the renal tissue during a long period of cold ischemia. Materials and methods: Oxidative changes were evaluated histologically and biochemically in kidneys from a total of 40 rats, which were assigned to 1 of 4 groups of 10 rats each: control group (no taurine given, kidneys evaluated immediately after removal), taurine group (as in the control group, except that taurine was given orally at a dose of 150 mg/kg per day for 3 days preceding kidney removal), ischemia group (no taurine given, kidneys evaluated after 72 h of ischemia at 4 °C in University of Wisconsin solution), and taurine plus ischemia group (as in the taurine group, except that kidneys subsequently underwent cold ischemia as in the ischemia group). Results: Compared to the ischemia group, the taurine plus ischemia group showed significantly lower levels of malondialdehyde and significantly higher activities of copper and zinc dependent superoxide dismutase, catalase, and glutathione peroxidase. Conclusion: Orally administered taurine appears to ameliorate the oxidative stress that occurs in kidneys during cold ischemia. Compared to the ischemia group, the taurine plus ischemia group had significantly less tubular necrosis, and glomerular and epithelial injury.

Anahtar Kelimeler:

Key words: Antioxidants, ischemia, kidney, taurine, transplantation

Taurine is protective against oxidative stress during cold ischemia in the rat kidney

Keywords:

Key words: Antioxidants, ischemia, kidney, taurine, transplantation,

PDF

___

Weinberg JM. Th e cell biology of ischemic renal injury. Kidney Int 1991; 39: 476-500.
Kosieradzki M, Rowiński W. Ischemia/reperfusion injury in kidney transplantation: mechanisms and prevention. Transplant Proc 2008; 40: 3279-88.
Paller MS. Free radical-mediated postischemic injury in renal transplantation. Ren Fail 1992; 14: 257-60.
Halliwell B, Whiteman M. Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean? Br J Pharmacol 2004; 142: 231-55.
Halliwell B. Biochemistry of oxidative stress. Biochem Soc Trans 2007; 35: 1147-50.
Michalk DV, Hoff mann B, Minor T. Taurine reduces renal ischemia/reperfusion injury in the rat. Adv Exp Med Biol 2003; 526: 49-56.
Guz G, Oz E, Lortlar N, Ulusu NN, Nurlu N, Demirogullari B et al. Th e eff ect of taurine on renal ischemia/reperfusion injury. Amino Acids 2007; 32: 405-11.
Guan X, Dei-Anane G, Liang R, Gross ML, Nickkholgh A, Kern M et al. Donor preconditioning with taurine protects kidney graft s from injury aft er experimental transplantation. J Surg Res 2008; 146: 127-34.
J Das, J Ghosh, P Manna, PC Sil. Taurine protects acetaminophen-induced oxidative damage in mice kidney through APAP urinary excretion and CYP2E1 inactivation. Toxicology 2010; 269: 24-34.
Bulucu F, Vural A, Aydın A, Sayal A. Oxidative stress status in adults with nephrotic syndrome. Clin Nephrol 2000; 53: 169- 173.
Yucel O, Uçar E, Tozkoparan E, Gunal A, Akay C, Sahin MA et al. Proanthocyanidin to prevent formation of the reexpansion pulmonary edema. J Cardiothor Surg 2009; 28: 40-8.
Aebi H. Catalase in vitro. In: Packer L (ed). Methods Enzymol. Orlando: Academic Press 1984; 105-26.
Aydın A, Orhan H, Sayal A, Özata M, Şahin G, Isımer A. Oxidative stress and nitric oxide related parameters in type II diabetes mellitus: eff ects of glycemic control. Clin Biochem 2001; 34: 65-70.
Norio K, Wikström M, Salmela K, Kyllönen L, Lindgren L. Ascorbic acid against reperfusion injury in human renal transplantation. Transpl Int 2003; 16: 480-5.
Green CJ, Healing G, Lunec J, Fuller BJ, Simpkin S. Evidence of free-radical induced damage in rabbit kidneys aft er simple hypothermic preservation and autotransplantation. Transplantation 1986; 41: 161-5.
Gower J, Fuller B, Green C. Lipid peroxidation in the cortex and medulla of rabbit kidneys subjected to cold ischaemia and the value of protective agents. Free Radic Res Commun 1987; 3: 107-15.
Gower JD, Healing G, Fuller BJ, SimpkinS, GreenCJ. Protection against oxidative damage in cold-stored rabbit kidneys by desferrioxamine and indomethacin. Cryobiology 1989; 26: 309-17.
Cotterill LA, Gower JD, Fuller BJ, Green CJ. Oxidative damage to kidney membranes during cold ischemia. Evidence of a role for calcium. Transplantation 1989; 48: 745-51.
Salahudeen AK, Huang H, Patel P, Jenkins JK. Mechanism and prevention of cold storage-induced human renal tubular cell injury. Transplantation 2000; 70: 1424-31.
Salahudeen AK. Cold ischemic injury of transplanted kidneys: new insights from experimental studies. Am J Physiol Renal Physiol 2004; 287: 181-7.
Aruoma OI, Halliwell B, Hoey BM, Butler J. Th e antioxidant action of taurine, hypotaurine and their metabolic precursors. Biochem J 1988; 256: 251-5.
Gaté L, Paul J, Ba GN, Tew KD, Tapiero H. Oxidative stress induced in pathologies: the role of antioxidants. Biomed Pharmacother 1999; 53: 169-80.
Mozaff ari M, Abdelsayed R, Patel C, Wimborne H, Liu JY, Schaff er SW. Diff erential eff ects of taurine treatment and taurine defi ciency on the outcome of renal ischemia reperfusion injury. J Biomed Sci 2010; 17(Suppl 1): 32.
Hansen SH, Andersen ML, Birkedal H, Cornett C, Wibrand F. Th e important role of taurine in oxidative metabolism. Adv Exp Med Biol 2006; 583: 129-35.
Salahudeen AK, Huang H, Joshi M, Moore NA, Jenkins JK. Involvement of the mitochondrial pathway in cold storage and rewarming-associated apoptosis of human renal proximal tubular cells. Am J Transplant 2003; 3: 273-80.