İbrahim PİRİM

8394

Sandwich-Type Enzyme Immunoassay for Amyloid A4 Protein in Cerebrospinal Fluid From Patients with Head Trauma

The aim of this study was to design a simple method for detecting the amyloid A4 protein in the cerebrospinal fluid (CSF) of patients who had severe brain trauma caused by traffic or other accidents and to evaluate its diagnostic and prognostic value. For this purpose, a sandwich type ELISA assay was used. A4, a 4kDa protein of 39-43 amino acids, is a metabolic product of a membrane-spanning, 695-770 aa, precursor molecule, the b-amyloid precursor protein (b-APP). The polyclonal rabbit antibody against synthetic A4 protein (1-40 residues) was used as an immobilized antibody. A mouse monoclonal antibody against synthetic A4 protein (1-28 residues) was used as a different immunoglobulin to attach the A4. Enzyme-labelled anti-immunoglobulin (peroxidase rabbit anti-mouse antibody, PRAM) was used for reaction with mouse immunoglobulin. The assay was highly specific for A4, demonstrating no cross reactivity between polyclonal anti A4 and monoclonal A4 antibodies (

Anahtar Kelimeler:

Beta-Amyloid protein (A4), Head Trauma, ELISA

Sandwich-Type Enzyme Immunoassay for Amyloid A4 Protein in Cerebrospinal Fluid From Patients with Head Trauma

The aim of this study was to design a simple method for detecting the amyloid A4 protein in the cerebrospinal fluid (CSF) of patients who had severe brain trauma caused by traffic or other accidents and to evaluate its diagnostic and prognostic value. For this purpose, a sandwich type ELISA assay was used. A4, a 4kDa protein of 39-43 amino acids, is a metabolic product of a membrane-spanning, 695-770 aa, precursor molecule, the b-amyloid precursor protein (b-APP). The polyclonal rabbit antibody against synthetic A4 protein (1-40 residues) was used as an immobilized antibody. A mouse monoclonal antibody against synthetic A4 protein (1-28 residues) was used as a different immunoglobulin to attach the A4. Enzyme-labelled anti-immunoglobulin (peroxidase rabbit anti-mouse antibody, PRAM) was used for reaction with mouse immunoglobulin. The assay was highly specific for A4, demonstrating no cross reactivity between polyclonal anti A4 and monoclonal A4 antibodies (

Keywords:

Beta-Amyloid protein (A4), Head Trauma, ELISA,

Turkish Journal of Medical Sciences-Cover

ISSN: 1300-0144
Yayın Aralığı: 6
Yayıncı: TÜBİTAK

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