Prevalence of blaIMP and blaVIM gene carriage in metallo-β lactamase-producing burn isolates of Pseudomonas aeruginosa in Tehran

Background/aim: To study the prevalence of blaVIM and blaIMP genes in metallo-β-lactamase (MBL) producing burn isolates of Pseudomonas aeruginosa in relation with AmpC and extended-spectrum β-lactamase (ESBL) production. Materials and methods: Thirty-two carbapenem-resistant MBL-producing P. aeruginosa burn isolates from Shahid Motahari Burn Hospital in Tehran were employed. Antibiotic susceptibility was determined to 13 antibiotics including imipenem and meropenem by disk diffusion. AmpC and ESBL production was detected by the AmpC disk test and combined disk diffusion assay, respectively. blaIMP and blaVIM gene carriage was shown by polymerase chain reaction and type-specific primers. Results: AmpC production was observed in 81% and ESBL production was detected in 12.5% of the isolates. blaIMP carriage was observed in 56.25% and blaVIM gene in 46.8% of the isolates. Surprisingly, 43.5% of the isolates carried both blaIMP and blaVIM genes. Conclusion: We think that this is the first report on the cocarriage of blaIMP and blaVIM in P. aeruginosa. There was also a strong association between MBL gene carriage and AmpC β-lactamase production

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1. Chakraborty D, Basu S, Das S. A study on infections caused by metallo-β-lactamase producing Gram negative bacteria in intensive care unit patients. Am J Infect Dis 2010; 6: 3439.
2. Kouda S, Kuwahara R, Ohara M, Shigeta M, Fujiwara T, Komatsuzawa H, Usui T, Sugai M. First isolation of blaIMP-7 in a Pseudomonas aeruginosa in Japan. J Infect Chemother 2007; 13: 276277.
3. Kohler T, Michea‐Hamzehpour M, Epp SF, Pechere JC. Carbapenem activities against Pseudomonas aeruginosa: respective contributions of OprD and efflux systems. Antimicrob Agent Chemother 1999; 43: 424427.
4. Livermore DM. Interplay of impermeability and chromosomal β-lactamase activity in imipenem-resistant Pseudomonas aeruginosa. Antimicrob Agent Chemother 1992; 36: 2046 2048.
5. Nordmann P, Poirel L. Emerging carbapenemases in Gramnegative aerobes. Clin Microbial Infect 2002; 8: 321331.
6. Pitout JDD, Gregson DB, Poirel L, McClure JA, Le P, Church DL. Detection of Pseudomonas aeruginosa producing metallo- β-lactamases in a large centralized laboratory. J Clin Microbiol 2005; 43: 31293135.
7. Rossolini GM. Acquired metallo-β-lactamases: an increasing clinical threat. Clin Infect Dis 2005; 41: 15571558.
8. Castanheira M, Toleman MA, Jones RN, Schmidt, FJ, Walsh TR. Molecular characterization of a β-lactamase gene, blaGIM-1, encoding a new subclass of metallo-β-lactamase. Antimicrob Agent Chemother 2004; 48: 46544661.
9. Walsh TR, Toleman MA, Poirel L, Nordmann P. Metallo-β- lactamases: the quiet before the storm? J Clin Microbiol Rev 2005; 18: 306325.
10. Salimi F, Eftekhar F. Coexistence of AmpC and extendedspectrum β-lactamases in metallo-β-lactamase producing Pseudomonas aeruginosa burn isolates in Tehran. Jundishapure J Microbiol 2013; 6: e7178.
11. Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-First Informational Supplement. Wayne, PA, USA: CLIS; 2011.
12. Thomson KS. Controversies about extended-spectrum and AmpC β-lactamases. Emerg Infect Dis 2002; 7: 333336.
13. Lee K, Kim CK, Hong SG, Choi J, Song S, Koh E, Yong D, Jeong SH, Yum JH, Docquier JD. Characteristics of clinical isolates of Acinetobacter genomospecies 10 carrying two different metallo-β-lactamases. Int J Antimicrob Agent 2010; 36: 259 253.
14. Yousefi S, Nahaei MR, Farajnia S, Ghojazadeh M, Akhi MT, Sharifi Y, Milani M, Ghotaslou R. Class 1 integron and imipenem resistance in clinical isolates of Pseudomonas aeruginosa: prevalence and antibiotic susceptibility. Iran J Microbiol 2010; 2: 113119.
15. Yousefi S, Farajnia S, Nahaei MR, Akhi MT, Ghotaslou R, Soroush MH, Naghili B, Hosseini Jazani N. Detection of metallo-β-lactamase encoding genes among clinical isolates of Pseudomonas aeruginosa in northwest of Iran. Diagn Microbiol Infect Dis 2010; 68: 322325.
16. Upadhyay S, Sen MR, Bhattacharjee A. Presence of different β-lactamase classes among isolates of Pseudomonas aeruginosa expressing AmpC β-lactamase enzyme. J Infect Dev Ctries 2010; 4: 239242.
17. Khosravi Y, Tay ST, Vadivelu J. Metallo-β-lactamase producing imipenem-resistant Pseudomonas aeruginosa clinical isolates in a university teaching hospital in Malaysia: detection of IMP- 7 and first identification of IMP-4, VIM-2, and VIM-11. Diagn Microbiol Infect Dis 2010; 67: 294296.
18. Bahar MA, Jamali SH, Samadikuchaksaraei A. Imipenemresistant Pseudomonas aeruginosa strains carry metallo-β- lactamase gene blaVIM in a level 1 Iranian burn hospital. Burns 2010; 36: 826830.
19. Jamali SH, Bahar MA, Houshmand SM. Detection of blaVIM among imipenem-resistant Pseudomonas aeruginosa isolated from burn wounds from Tehran Shahid Motahari Hospital. J Microbiol Knowl 2009; 1: 1925.
20. Sadeghi A, Rahimi B, Shojapour M. Molecular detection of metallo-β-lactamase genes blaVIM-1, blaVIM-2, blaIMP-1, blaIMP-2 and blaSPM-1 in Pseudomonas aeruginosa isolated from hospitalized patients in Markazi province by Duplex-PCR. Afr J Microbiol 2012; 6: 29652969.
21. Saderi H, Lotfalipour H, Owlia P, Salimi H. Detection of metallo-β-lactamase producing Pseudomonas aeruginosa from burn patients in Tehran, Iran. Lab Medicine 2010; 41: 609612.
22. Shahcheraghi F, Nikbin VS, Feizabadi MM. Identification and genetic characterization of metallo-β-lactamase producing strains of Pseudomonas aeruginosa in Tehran, Iran. New Microbiol 2010; 33: 243248.
23. Dwivedi M, Mishra A, AzimA, Singh RK, Baronia AK, Prasad KN, Dhole TN, Dwivedi UN. Ventilator-associated pneumonia caused by carbapenem-resistant Enterobacteriaceae carrying multiple metallo-β-lactamase genes. Ind J Pathol Microbiol 2009; 52: 339342.
24. Lagatolla C, Edalucci E, Dolzani L, Riccio ML, De Luca F, Medessi E, Rossolini GM, Tonin EA. Molecular evolution of metallo-β-lactamase producing Pseudomonas aeruginosa in a nosocomial setting of high level endemicity. J Clin Microbiol 2006; 44: 23482353.
25. Aktaş Z, Kayacan ÇB. Investigation of metallo-β-lactamase producing strains of Pseudomonas aeruginosa and Acinetobacter baumannii by E-test, disk synergy, and PCR. Scand J Infect Dis 2008; 40: 320325.
26. Chu YW, Cheung TK, Ngan JY, Kam KM. EDTA susceptibility leading to false detection of metallo-β-lactamase in Pseudomonas aeruginosa by E-test and an imipenem-EDTA disk method. Int J Antimicrob Agent 2005; 26: 340341.