Konstantsa TOSHEVA-DASKALOVA, Tanya Vasileva STRATEVA, İvan Gergov MITOV, Raina Tzvetanova GERGOVA

Multiplex PCR detection of problematic pathogens of clinically heterogeneous bacterial vaginosis in Bulgarian women

Öz Background/aim: This study aimed to investigate the correlation between the prevalence of problematic pathogens and the clinical status of women with bacterial vaginosis (BV). Materials and methods: Gardnerella vaginalis, Atopobium vaginae, and Mobiluncus spp. were detected using a multiplex PCR assay, and their role in the infection of Bulgarian women with clinically heterogeneous BV was evaluated. Results: The predominant BV-associated pathogen identified was G. vaginalis with an incidence of 98.39%, followed by A. vaginae (68.05%) and Mobiluncus spp. at 17.01%. The coexistence of A. vaginae and G. vaginalis was more common in women with discharge (in 72.04%) and in patients with chronic recurrent BV than among asymptomatic or newly diagnosed BV cases (P < 0.05). Mobiluncus spp. was detected mostly in coinfections, in association with Trichomonas vaginalis. The coinfections were predominantly related to recurrent BV and with complications (P < 0.05). Conclusion: This is the first study about the correlation between problematic pathogens and clinically heterogeneous BV in Bulgarian women. High frequency of infection with key BV-related pathogens was observed in childbearing women. The incidence was shown to often correlate with coexistent T. vaginalis, with severity of infection, and with complicated and recurrent BV after unsuccessful treatments. Screening should be considered in reproductive health programs.

Anahtar Kelimeler:

Bacterial vaginosis, PCR, Gardnerella vaginalis, Atopobium vaginae, Mobiluncus spp.

Tam Metin

Kaynak Göster

Bibtex	`@ { tbtkmedical576378, journal = {Turkish Journal of Medical Sciences}, issn = {1300-0144}, eissn = {1303-6165}, address = {}, publisher = {TÜBİTAK}, year = {2017}, volume = {47}, pages = {1492 - 1499}, doi = {}, title = {Multiplex PCR detection of problematic pathogens of clinically heterogeneous bacterial vaginosis in Bulgarian women}, key = {cite}, author = {Tosheva-daskalova, Konstantsa and Strateva, Tanya Vasileva and Mıtov, İvan Gergov and Gergova, Raina Tzvetanova} }`
APA	Tosheva-daskalova, K , Strateva, T , Mıtov, İ , Gergova, R . (2017). Multiplex PCR detection of problematic pathogens of clinically heterogeneous bacterial vaginosis in Bulgarian women . Turkish Journal of Medical Sciences , 47 (5) , 1492-1499 . Retrieved from https://dergipark.org.tr/tr/pub/tbtkmedical/issue/45689/576378
MLA	Tosheva-daskalova, K , Strateva, T , Mıtov, İ , Gergova, R . "Multiplex PCR detection of problematic pathogens of clinically heterogeneous bacterial vaginosis in Bulgarian women" . Turkish Journal of Medical Sciences 47 (2017 ): 1492-1499 <https://dergipark.org.tr/tr/pub/tbtkmedical/issue/45689/576378>
Chicago	Tosheva-daskalova, K , Strateva, T , Mıtov, İ , Gergova, R . "Multiplex PCR detection of problematic pathogens of clinically heterogeneous bacterial vaginosis in Bulgarian women". Turkish Journal of Medical Sciences 47 (2017 ): 1492-1499
RIS	TY - JOUR T1 - Multiplex PCR detection of problematic pathogens of clinically heterogeneous bacterial vaginosis in Bulgarian women AU - Konstantsa Tosheva-daskalova , Tanya Vasileva Strateva , İvan Gergov Mıtov , Raina Tzvetanova Gergova Y1 - 2017 PY - 2017 N1 - DO - T2 - Turkish Journal of Medical Sciences JF - Journal JO - JOR SP - 1492 EP - 1499 VL - 47 IS - 5 SN - 1300-0144-1303-6165 M3 - UR - Y2 - 2021 ER -
EndNote	%0 Turkish Journal of Medical Sciences Multiplex PCR detection of problematic pathogens of clinically heterogeneous bacterial vaginosis in Bulgarian women %A Konstantsa Tosheva-daskalova , Tanya Vasileva Strateva , İvan Gergov Mıtov , Raina Tzvetanova Gergova %T Multiplex PCR detection of problematic pathogens of clinically heterogeneous bacterial vaginosis in Bulgarian women %D 2017 %J Turkish Journal of Medical Sciences %P 1300-0144-1303-6165 %V 47 %N 5 %R %U
ISNAD	Tosheva-daskalova, Konstantsa , Strateva, Tanya Vasileva , Mıtov, İvan Gergov , Gergova, Raina Tzvetanova . "Multiplex PCR detection of problematic pathogens of clinically heterogeneous bacterial vaginosis in Bulgarian women". Turkish Journal of Medical Sciences 47 / 5 (Ekim 2017): 1492-1499 .
AMA	Tosheva-daskalova K , Strateva T , Mıtov İ , Gergova R . Multiplex PCR detection of problematic pathogens of clinically heterogeneous bacterial vaginosis in Bulgarian women. Turkish Journal of Medical Sciences. 2017; 47(5): 1492-1499.
Vancouver	Tosheva-daskalova K , Strateva T , Mıtov İ , Gergova R . Multiplex PCR detection of problematic pathogens of clinically heterogeneous bacterial vaginosis in Bulgarian women. Turkish Journal of Medical Sciences. 2017; 47(5): 1492-1499.
IEEE	K. Tosheva-daskalova , T. Strateva , İ. Mıtov ve R. Gergova , "Multiplex PCR detection of problematic pathogens of clinically heterogeneous bacterial vaginosis in Bulgarian women", Turkish Journal of Medical Sciences, c. 47, sayı. 5, ss. 1492-1499, Eki. 2017