Is there a role of interstitial cells of Cajal and mast cells and eosinophilsin appendicitis in children?
Background/aim: The aim of this study was to compare the distribution of interstitial Cajal cells, eosinophils, and mast cells in normal and inflamed appendices, and to evaluate the correlation of presence of these cells with severity of inflammation in appendicitis. Materials and methods: The appendicitis group (n = 30) was divided further into three groups according to the macroscopic description and the histological findings. Ten normal appendices served as controls. Tissue samples were processed for routine histological examination. Additionally, all sections were immunohistochemically stained with CD117 and mast cell tryptase antibodies. Results: When specimens were compared in terms of Cajal cells, the observed mean number for the appendicitis group was 4.9 and for the control group it was 8.3. In contrast, eosinophils and mast cells were significantly increased in the appendicitis group when compared with the control group. Conclusion: We detected that eosinophils and mast cells are increased in appendicitis, and correlate with the degree of inflammation of the appendix. The density of interstitial Cajal cells was significantly lower in patients with severe appendix inflammation compared to controls. The histopathological differences observed in this study may help elucidate the pathophysiology of appendicitis.
Is there a role of interstitial cells of Cajal and mast cells and eosinophilsin appendicitis in children?
Background/aim: The aim of this study was to compare the distribution of interstitial Cajal cells, eosinophils, and mast cells in normal and inflamed appendices, and to evaluate the correlation of presence of these cells with severity of inflammation in appendicitis. Materials and methods: The appendicitis group (n = 30) was divided further into three groups according to the macroscopic description and the histological findings. Ten normal appendices served as controls. Tissue samples were processed for routine histological examination. Additionally, all sections were immunohistochemically stained with CD117 and mast cell tryptase antibodies. Results: When specimens were compared in terms of Cajal cells, the observed mean number for the appendicitis group was 4.9 and for the control group it was 8.3. In contrast, eosinophils and mast cells were significantly increased in the appendicitis group when compared with the control group. Conclusion: We detected that eosinophils and mast cells are increased in appendicitis, and correlate with the degree of inflammation of the appendix. The density of interstitial Cajal cells was significantly lower in patients with severe appendix inflammation compared to controls. The histopathological differences observed in this study may help elucidate the pathophysiology of appendicitis.
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- Ergul E. Heredity and familial tendency of acute appendicitis. Scand J Surg 2007; 96: 290–302. 2. Bettolli M, De Carli C, Cornejo-Palma D, Jolin-Dahel K, Wang XY, Huizinga J, Krantis A, Rubin S, Staines WA. Interstitial cell of Cajal loss correlates with the degree of inflammation in the human appendix and reverses after inflammation. J Pediatr Surg 2012; 47: 1891–1899.
- Richter A, Wit C, Vanderwinden JM, Wit J, Barthlen W. Interstitial cells of Cajal in the vermiform appendix in childhood. Eur J Pediatr Surg 2009; 19: 30–33.
- Singh UR, Malhotra A, Bhatia A. Eosinophils, mast cells, nerves and ganglion cells in appendicitis. Indian J Surg 2008; 70: 231–234.
- Ruffolo C, Fiorot A, Pagura G, Antoniutti M, Massani M, Caratozzolo E, Bonariol L, Calia di Pinto F, Bassi N. Acute appendicitis: what is the gold standard of treatment? World J Gastroenterol 2013; 19: 8799–8807.
- Kim JS. Acute abdominal pain in children. Pediatr Gastroenterol Hepatol Nutr 2013; 16: 219–224. 7. Lee H, Park JH, Park DI, Kim HJ, Cho YK, Sohn CI, Jeon WK, Kim BI, Chae SW. Mucosal mast cell count is associated with intestinal permeability in patients with diarrhea predominant irritable bowel syndrome. J Neurogastroenterol Motil 2013; 19: 244–250.
- Jakate S, Demeo M, John R, Tobin M, Keshavarzian A. Mastocytic enterocolitis: increased mucosal mast cells in chronic intractable diarrhea. Arch Pathol Lab Med 2006; 130: 362–367. 9. Mysorekar VV, Chanda S, Dandeka CP. Mast cells in surgically resected appendices. Indian J Pathol Microbiol 2006; 49: 229– 333.
- Coşkun N, Indel SM, Elpek GO. Mast cell density and neuronal hypertrophy in patients with acute appendicitis. Turk J Gastroenterol 2003; 14: 54–58.