Barbara KOT, Jolanta WICHA, Malgorzata PIECHOTA, Katarzyna WOLSKA, Agata GRUZEWSKA

Antibiofilm activity of trans-cinnamaldehyde, p-coumaric, and ferulic acids on uropathogenic Escherichia coli

Background/aim: Biofilm on urinary catheters results in persistent infections that are resistant to antibiotics. In this study, phytochemicals were assessed as alternative antimicrobials in preventing and inactivating E. coli biofilm on urinary catheters. Materials and methods: Biofilm prevention was tested using catheter fragments inoculated with E. coli and treated with trans-cinnamaldehyde, p-coumaric, and ferulic acids (0%, 0.1%, 0.25%, and 0.5%) for 0, 1, 3, and 5 days. Inactivation of E. coli biofilm with the same agents at concentrations of 0%, 1%, 1.25%, or 1.5% used for 0, 1, 3, or 5 days was also evaluated. Results: All used concentrations of trans-cinnamaldehyde prevented and effectively inactivated E. coli biofilm formed on urinary catheter fragments. p-Coumaric (0.25% and 0.5%) and ferulic acids (0.5%) had preventive action on E. coli biofilm formation in urinary catheter fragments. The number of uropathogenic E. coli cells in biofilm formed in the lumen of a urinary catheter was significantly reduced in the presence of p-coumaric and ferulic acids, but complete inactivation of the biofilm formed was not observed, as opposed to the use of trans-cinnamaldehyde. Conclusion: The obtained results indicate that phytochemicals may be an important source of antibiofilm agents that have preventive action on E. coli biofilm formation on urinary catheters.

Anahtar Kelimeler:

Uropathogenic Escherichia coli, biofilm, cinnamic aldehyde, coumaric acid, ferulic acid

Antibiofilm activity of trans-cinnamaldehyde, p-coumaric, and ferulic acids on uropathogenic Escherichia coli

Keywords:

Uropathogenic Escherichia coli, biofilm, cinnamic aldehyde, coumaric acid, ferulic acid,

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1. Jacobsen SM, Stickler DJ, Mobley HLT, Shirtliff ME. Complicated catheter-associated urinary tract infections due to Escherichia coli and Proteus mirabilis. Clin Microbiol Rev 2008; 21: 26–59.
2. Foxman B. Epidemiology of urinary tract infections: incidence, morbidity, and economic costs. Dis Mon 2003; 49: 53–70. 3. Nicolle LE. Catheter-related urinary tract infection. Drugs Aging 2005; 22: 627–639.
4. Trautner BW, Darouiche RO, Hull RA, Hull S, Thornby JI. Preinoculation of urinary catheters with Escherichia coli 83972 inhibits catheter colonization by Enterococcus faecalis. J Urol 2002; 167: 375–379.
5. Tambyah PA. Catheter-associated urinary tract infections: diagnosis and prophylaxis. Int J Antimicrob Agents 2004; 24S: 44–48.
6. Ha US, Cho YH. Catheter-associated urinary tract infections: new aspects of novel urinary catheters. Int J Antimicrob Agents 2006; 28: 458–490.
7. Desai DG, Liao KS, Cevallos ME, Trautner BW. Silver or nitrofurazone impregnation of urinary catheters has a minimal effect on uropathogen adherence. J Urol 2010; 184: 2565–2571.
8. Dixon RA. Natural products and plant disease resistance. Nature 2001; 411: 843–847.
9. Russell W, Duthie G. Plant secondary metabolites and gut health: the case for phenolic acids. Proc Nutr Soc 2011; 70: 389–396.
10. Dai J, Mumper RJ. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 2010; 15: 7313–7352.
11. Mussatto SI, Dragone G, Roberto IC. Ferulic and p-coumaric acids extraction by alkaline hydrolysis of brewer’s spent grain. Ind Crop Prod 2007; 25: 231–237.
12. Burt S. Essential oils: their antibacterial properties and potential application in foods–a review. Int J Food Microbiol 2004; 94: 223–253.
13. D’Archivio M, Filesi C, Di Benedetto R, Gargiulo R, Giovannini C, Masella R. Polyphenols, dietary sources and bioavailability. Ann Ist Super Sanita 2007; 43: 348–361.
14. Amalaradjou MAR, Narayanan A, Baskaran SA, Venkitanarayanan K. Antibiofilm effect of transcinnamaldehyde on uropathogenic Escherichia coli. J Urol 2010; 184: 358–363.
15. Amalaradjou MAR, Narayanan A, Venkitanarayanan K. Trans-cinnamaldehyde decreases attachment and invasion of uropathogenic Escherichia coli in urinary tract epithelial cells by modulating virulence gene expression. J Urol 2011; 185: 1526–1531.
16. Koneman EW, Allen SD, Janda WM, Schreckenberger PC, Winn WC. Color Atlas and Textbook of Diagnostic Microbiology. 5th ed. Philadelphia, PA, USA: Lippincott-Raven; 1997.
17. Trautner BW, Hull RA, Darouiche RO. Escherichia coli 83972 inhibits catheter adherence by a broad spectrum of uropathogenes. Urology 2003; 61: 1059–1062.
18. Dellimore KH, Helyer AR, Franklin SE. A scoping review of important urinary catheter induced complications. J Mater Sci: Mater Med 2013; 24: 1825–1835.
19. Lee SJ, Kim SW, Cho YH, Shin WS, Lee SE, Kim CS, Hong SJ, Chung BH, Kim JJ, Yoon MS. A comparative multicentre study on the incidence of catheter-associated urinary tract infection between nitrofurazone-coated and silicone catheters. Int J Antimicrob Agents 2004; 24S: 65–69.
20. Regev-Shoshani G, Ko M, Crowe A, Av-Gay Y. Comparative efficacy of commercially available and emerging antimicrobial urinary catheters against bacteriuria caused by Escherichia coli in vitro. Urology 2011; 78: 334–339.
21. Trautner BW, Darouiche RO. Role of biofilm in catheterassociated urinary tract infection. Am J Infect Control 2004; 32: 177–183.
22. Domadia P, Swarup S, Bhunia A, Sivaraman J, Dasgupta D. Inhibition of bacterial cell division protein FtsZ by cinnamaldehyde. Biochem Pharmacol 2007; 832: 831–840.
23. Lou Z, Wang H, Rao S, Sun J, Ma C, Li J. p-Coumaric acid kills bacteria through dual damage mechanisms. Food Control 2012; 25: 550–554.
24. Alves MJ, Ferreira ICFR, Froufe HJC, Abreu RMV, Martins A, Pintado M. Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies. J Appl Microbiol 2013; 115: 346–357.
25. Borges A, Saavedra MJ, Simões M. The activity of ferulic and gallic acids in biofilm prevention and control of pathogenic bacteria. Biofouling 2012; 28: 755–767.
26. Borges A, Ferreira C, Saavedra MJ, Simões M. Antibacterial activity and mode of action of ferulic and gallic acids against pathogenic bacteria. Microb Drug Resist 2013; 19: 256–265.