Oya SAYIN, Zekiye Sultan ALTUN, Gül AKDOĞAN, Nur ARSLAN

In vitro eff ect of resveratrol against oxidative injury of human coronary artery endothelial cells

Amaç: Reaktif oksijen ürünleri endotel fonksiyon kaybına katkıda bulunabilen oksidatif stresi indüklemektedir. Endojen antioksidanların yanı sıra, diyetle antioksidanların alımı, endotel fonksiyon kaybını indükleyen oksidatif stresi azaltmaktadır. Resveratrol antioksidan aktiviteyi kapsayan birçok etkilere sahip doğal bir fi toaleksindir. Bu çalışmanın amacı resveratrolün in vitro oksidatif stresin aracılık ettiği hasara karşı insan koroner arter endotel hücrelerini koruyucu etkisi olup olmadığının araştırılmasıdır. Yöntem ve gereç: İnsan koroner arter endotel hücreleri farklı süre ve konsantrasyonlarda hidrojen peroksitle ve hidrojen peroksit + resveratrol ile muamele edildi. Daha sonra hücre ölümü laktat dehidrogenaz salınımı ile ölçüldü. İnsan koroner arter endotel hücreleri hidrojen peroksite ve hidrojen peroksit + resveratrole maruz bırakıldı. 15., 60. dakikada ve 6. saatte reaktif oksijen ürünleri fl orometrik ölçümle değerlendirildi. Bulgular: 10 ve 50 μM resveratrol ile farklı zaman noktalarında preinkübasyon, hidrojen peroksit ile indüklenen hücre ölümünün anlamlı olarak azalmasına neden olmaktadır. Resveratrol reaktif oksijen ürünleri oluşumunda anlamlı azalmaya neden olmaktadır. Sonuç: Bulgularımız, resveratrolün, in vitro insan koroner arter endotel hücrelerinde reaktif oksijen ürünleri oluşumunu ve hücre ölümünü azaltarak oksidatif stresin indüklediği hücre hasarına karşı koruyucu etkisinin olduğunu göstermektedir.

Resveratrolün insan koroner arter endotel hücrelerinde oksidatif hücre hasarina karşı in vitro etkisi

Aim: Reactive oxygen species induce oxidative stress that may contribute to endothelial dysfunction. In addition to endogen antioxidants, adding antioxidants to diet has been used to reduce oxidative-stress-induced endothelial dysfunction. Resveratrol is a natural phytoalexin which has many eff ects including antioxidant activity. Th e aim of this study was to investigate whether resveratrol could protect human coronary artery endothelial cells against in vitro oxidative-stress-mediated injury. Materials and methods: Human coronary artery endothelial cells were treated with varying concentrations of hydrogen peroxide for diff erent durations and hydrogen peroxide plus varying concentrations of resveratrol. Th en cell death was measured using lactate dehydrogenase release. Human coronary artery endothelial cells were exposed to hydrogen peroxide and resveratrol plus hydrogen peroxide for 15 min, 60 min, and 6 h. Reactive oxygen species was measured using a fl uorometric assay. Results: Preincubation of cells with 10 and 50 μM resveratrol for diff erent periods caused a signifi cantly decreased hydrogen-peroxide-induced cell death. Preincubation with resveratrol caused a signifi cant decrease in reactive oxygen species production. Conclusion: Our results demonstrate that resveratrol protects human coronary artery endothelial cells, in vitro, against oxidative-stress-induced injury by decreasing cell death and reactive oxygen species production.

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