Alper ÇAĞLAR, Cumhur SİPAHİ, Mehmet DALKIZ, Jülide ÖZEN

In vitro Cytotoxicity of Glass and Carbon Fiber-Reinforced Heat-Polymerized Acrylic Resin Denture Base Material

Acrylic resin dentures may have cytotoxic effects on oral tissues. However, there is sparse data about the cytotoxic effect of fiber-reinforced acrylic resin materials. The aim of this in vitro study was to determine the cytotoxicity of a heat-polymerized acrylic resin denture base material reinforced with glass, carbon fibers and unreinforced acrylic resin denture base material. 100 acrylic resin discs were assigned to 5 experimental groups (n=20) and 1 control group. Group NOF did not receive any fiber. Roving type glass (Vetrolex) and carbon fibers (Type Tenox J) were introduced into the acrylic resin specimens of Group RGF and Group RCF with the "embedding" technique. Particulate glass and carbon fibers were impregnated into the specimens of Group PGF and PCF with the "blending" technique. Gingival fibroblast cells were exposed onto the test specimens and the cytotoxicity was determined by succinic dehydrogenase activity (MTT method) after 24 and 72 hours incubation. Data were analyzed with the 1-way-Anova test. Cell proliferation rates after 24 and 72 hours incubation ranged as follows: NOF (89%-83%)> RGF (80%-77%), PGF (80%-76%), RCF (79%-75%), PCF (77%-73%). All groups displayed significant cytotoxicity compared to control cell culture. Fiber reinforced groups were significantly more cytotoxic than the unreinforced group. No significant difference of cytotoxicity was found between the fiber-reinforced groups.

Anahtar Kelimeler:

Cytotoxity, denture base materials, glass fiber, carbon fiber, cell culture

In vitro Cytotoxicity of Glass and Carbon Fiber-Reinforced Heat-Polymerized Acrylic Resin Denture Base Material

Keywords:

Cytotoxity, denture base materials, glass fiber, carbon fiber, cell culture,

PDF

___

Brown LR, Flavin C, French H. A new economy for a new century. In: State of the World. New York: W.W. Norton & Company, 5- 7, 1999.
Lassila LVJ, Vallittu PK. Denture base polymer Alldent Sinomer: mechanical properties, water sorption and release of residual compounds. J Oral Rehabil 28: 607-613, 2001. 3. Danilewitz Z. Experimental investigations on the cytotoxic nature of methyl methacrylate. J Prosthet Dent 44: 13-16, 1980. 4. Tsuchiya H, Hoshino Y, Tajima K et al. Leaching and cytotoxicity
of formaldehyde and methyl methacrylate from acrylic resin
denture base materials. J Prosthet Dent 71: 618-624, 1994. 5. Sadamori S, Kotani H, Hamada T. The usage period of dentures and their residual monomer contents. J Prosthet Dent 68: 374- 376, 1992.
Baker S, Brooks SC, Walker DM. The release of residual monomeric methyl methacrylate from acrylic appliances in the human mouth: An assay for monomer in saliva. J Dent Res 67:1295-1299, 1988.
Koda T, Tsuchiya H, Yamauchi M et al. Leachability of denture base acrylic resins in artificial saliva. Dent Mater 6: 13-16, 1990.
Vallittu PK, Ruyter IE, Buykuilmaz S. Effect of polymerization temperature and time on the residual monomer content of denture base polymers. Eur J Oral Sci 106: 588-93, 1998.
Cimpan MR, Matre R, Cressey LI et al. The effect of heat and auto-polymerized denture base polymers on clonogenicity, apoptosis, and necrosis in fibroblasts: denture base polymers induce apoptosis and necrosis. Acta Odont Scand 58: 217-228, 2000.
Sadamori S, Ganefiyanti T, Hamada T et al. Influence of thickness and location on the residual monomer content of denture base cured by three processing methods. J Prosthet Dent 72: 19-22, 1994.
Sheridan PJ, Koka S, Ewoldsen NO et al. Cytotoxicity of denture base resins. Int J Prosthodont 10: 73-77, 1997.
Lefebvre CA, Schuster GS, Marr JC et al. The effect of pH on the cytotoxicity of eluates from denture base resins. Int J Prosthodont 8: 122-128, 1995.
Huang FM, Tai KW, Hu CC et al. Cytotoxic effects of denture base materials on a permanent human oral epithelial cell line and on primary human oral fibroblasts in vitro. Int J Prosthodont 14: 439-443, 2001.
Miettinen VM, Vallittu PK. Release of residual methyl methacrylate into water from glass fiber-poly-methl methacrylate composite used in dentures. Biomaterials. 11: 340- 350, 1997.
Yilmaz H, Aydin C, Caglar A et al. The effect of glass fiber reinforcement on the residual monomer content of two denture base resins. Quintessence Int 34: 148-153, 2003.
Vallittu PK, Ekstrand K. In vitro cytotoxicity of fibre-polymethyl methacrylate composite used in dentures. J Oral Rehabil 26: 666- 671, 1999.
Ekstrand K, Ruyter IE, Wellendorf H. Carbon graphite fiber reinforced poly(methyl methacrylate): properties under dry and wet conditions. J Biomed Mater Res 21: 1065, 1987.
Vallittu PK. Acrylic resin-fiber composite- Part II: The effect of polymerization shrinkage of polymethyl methacrylate applied to fiber roving on transverse strength. J Prosthet Dent 71: 613- 617, 1994.
Vallittu PK. The effect of void space and polymerization time on transverse strength of acrylic-glass fiber composite. J Oral Rehabil 22: 257-261, 1995.
Vallittu PK. Impregnation of glass fiber with polymethyl methacrylate by using a powder coating method. Appl Composite Mater 2: 51-58, 1995.
Vallittu PK, Lassila VP, Lappalainen R. Transverse strength and fatigue of denture acrylic-glass fiber composite. Dent Mater 10: 116-121, 1994.
Ladizesky NH, Ho CF, Chow TW. Reinforcement of complete denture bases with continuous high performance polyethylene fibers. J Prosthet Dent 63: 934-939, 1992.
Vallittu PK. Flexural properties of acrylic resin polymers reinforced with unidirectional or woven glass fibers. J Prosthet Dent 81; 318-326, 1999.
Vallittu PK, Ruyter IE, Ekstrand K. Effect of water storage on the flexural properties of E-glass and silica fibers acrylic resin composite. Int J Prosthodont 11:340-350, 1998.
Bumgardner JD, Lucas LC, Tilden AB. Toxicity of copper-based dental alloys in cell culture. J Biomed Mater Res 23: 1103-14, 1989.
Tardif F, Ross G, Rouabhia M. Gingival and dermal fibroblasts produce interleukin-1βconverting enzyme and interleukin-1βbut not interleukin-18 even after stimulation with lipopolysaccharide. J Cell Physiol 198: 125-132, 2004.
Ozen J, Atay A, Beydemir B et al. In vitro IL-1βrelease from gingival fibroblasts in response to pure metals, dental alloys and ceramic. J Oral Rehabil 31: 1-7, 2004.
Wataha JC, Craig RG, Hanks CT. Precision of and new methods for testing in vitro alloy cytotoxicity. Dent Mater 8: 65, 1992.
Edmondson JM, Armstrong LS, Martinez AO. A rapid and single MTT based spectrophotometric assay for determination of drug sensitivity in monolayer cultures. J Tissue Culture Methods 11: 15-17, 1988.
Nakamura M, Kawahara H. Long-term biocompatibility test of denture base resins in-vitro. J Prosthet Dent 52: 694-699, 1984;.
Harrison A, Hugget R. Effect of curing cycle on residual monomer levels of acrylic resin denture base polymers. J Prosthet Dent 20: 370-374, 1992.
Gough JE, Downes S, Osteoblast cell death on methacrylate polymers involves apoptosis. J Biomed Mater Res 57; 497-505, 2001.
Yunus N, Rashid AA, Azmi LL et al. Some flexural properties of a nylon denture base polymer. J Oral Rehabil 32:65-71, 2005.