Does capsaicin have therapeutic benefits in human colon adenocarcinoma? Selection of the most reliable dose via AgNOR

Background/aim: To determine the effect of different doses of capsaicin on AgNOR protein synthesis in human colon adenocarcinoma derivate from colon cancer Caco-2 cell . Materials and methods: In this experimental study, after the cultured of Caco-2 cell line, the cells are divided into 4 groups as control and different capsaicin exposed doses 25uµ, 50uµ, and 75uµ . Mean AgNOR number and total AgNOR area/nuclear area TAA/NA were calculated. Results: A significant differences were detected between control and capsaicin 50uµ P = 0.001 , between control and capsaicin 75uµ P = 0.000 , between capsaicin 25uµ and capsaicin 50uµ P = 0.001 and between capsaicin 25uµ and capsaicin 75uµ P = 0.000 for TAA/NA. Also, there were significant differences between control and capsaicin 50uµ P = 0.001 , between control and capsaicin 75uµ P = 0.000 , between capsaicin 25uµ and capsaicin 50uµ P = 0.000 and between capsaicin 25uµ and capsaicin 75uµ P = 0.000 for mean AgNOR number. Conclusion: A certain amount of capsaicin has a protective effect against colon adenocarcinoma and the dose concentrations are important for the most reliable treatment.

Keywords:

AgNOR, capsaicin, human colon adenocarcinoma rDNA, caco-2 cell line,

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