Cytomorphological findings in diagnosis of Warthin tumor
Cytomorphological findings in diagnosis of Warthin tumor
Background/aim: To define the cytomorphologic findings leading to difficulties in diagnosis of Warthin tumors (WTs).Materials and methods: Forty-eight histopathologically diagnosed WT patients who had fine needle aspiration cytology preoperativelywere reevaluated for defining the presence or absence of lymphocytes, oncocytic cell layer, oncocytic cell papillae, granular debrisbackground, mucoid background, macrophages, polymorphonuclear cells, mast cells, squamous-like cells, atypical vacuolatedcytoplasmic cells, and giant cells.Results: Forty-seven tumors were in the parotid gland and one in the submandibular gland. There were 37 (77%) male and 11 (23%)female patients. Cytopathologically in 36 patients the diagnosis was benign neoplasm (WT); in 6, other benign entities; and in 6,suspicious for malignancy. The main characteristic cytomorphologic features of WTs were as follows: 92% lymphoid cells, 83% oncocyticcell layers, and 67% granular debris background. These percentages were 67%, 17%, and 17% in the benign cytology group and 67%,50%, and 17% in the suspicious for malignancy group, respectively.Conclusion: Absence or lack of main features of WTs with or without presence of squamous-like cells, vacuolated cytoplasmic cells,and inflammatory reaction may cause diagnostic dilemma. The presence of the mast cells accompanied by epithelial tissue was strikingfor WT diagnosis.
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- 1. Jung SM, Hao SP. Warthin’s tumor with multiple granulomas: a
clinicopathologic study of six cases. Diagnostic Cytopathology
2006; 34 (8): 564-567. doi: 10.1002/dc.20506
- 2. Bajaj P, Garg D, Sabharwal R, Gautam S. Fine needle
aspiration cytology in Warthin’s tumor: a diagnostic tool.
Diagnostic Pathology: Open Access 2015; 1 (1): 102-106. doi:
10.4172/2476-2024.1000102.
- 3. Mukunyadzi P. Review of fine-needle aspiration cytology
of salivary gland neoplasms, with emphasis on differential
diagnosis. Pathology Patterns Reviews 2002; 118 (1): 100-115.
doi: 10.1309/WVVR-30E4-13TW-494D
- 4. Fležar M, Pogačnik A. Warthin’s tumour: unusual vs. common
morphological findings in fine needle aspiration biopsies.
Cytopathology 2002; 13 (4): 232-241. doi: 10.1046/j.1365-
2303.2002.00415.x
- 5. Pantanowitz L, Thompson LDR, Rossi ED. Diagnostic
approach to fine needle aspirations of cystic lesions of the
salivary gland. Head Neck Pathology 2018; 12 (4): 548-561.
doi: 10.1007/s12105-018-0904-8
- 6. Parwani AV, Syed A. Diagnostic accuracy and pitfalls in fineneedle
aspiration interpretation of Warthin tumor. Cancer
2003; 99 (3): 166-171. doi: 10.1002/cncr.11207
- 7. Sood N, Borah P. Warthins tumor: Cytohistological spectrum
with emphasis on diagnostic difficulties. Diagnostic
Cytopathology 2018; 46 (7): 613-619. doi: 10.1002/dc.23896
- 8. Shetty A, Geethamani V. Role of fine-needle aspiration
cytology in the diagnosis of major salivary gland tumors: a
study with histological and clinical correlation. Journal of
Oral and Maxillofacial Pathology 2016; 20 (2): 224-229. doi:
10.4103/0973-029X.185899
- 9. Kuzenko YV, Romanuk AM, Dyachenko OO, Hudymenko O.
Pathogenesis of Warthin’s tumors. Interventional Medicine &
Applied Science 2016; 8 (2): 41-48. doi: 10.1556/1646.8.2016.2.2
- 10. Stelow EB. Updates in salivary gland fine needle aspiration
biopsy the use of the Milan system and ancillary testing.
Surgical Pathology 2018; 11 (3): 489-500. doi: 10.1016/j.
path.2018.04.003
- 11. Viguer JM, Vicandi B, Heffernan JAJ, Ferrer PL, Peramato PG
et al. Role of fine needle aspiration cytology in the diagnosis
and management of Warthin’s tumour of the salivary glands.
Cytopathology 2010; 21 (3): 164-169. doi: 10.1111/j.1365-
2303.2009.00667.x
- 12. Kim JY, Yoo YS, Kwon JE, Kim HE, Park K. Fine-needle
aspiration cytology with c-kit immunocytochemical staining
in the diagnosis of Warthin’s tumor. Acta Cytologica 2012; 56
(5): 474-480. doi: 10.1159/000339195
- 13. Klijanienko J, Vielh P. Fine needle sampling of salivary
gland lesions II. Cytology and histology correlation of 71
cases of Warthin’s tumor (adenolymphoma). Diagnostic
Cytopathology 1997; 16 (3): 221-225. doi: 10.1002/(sici)1097-
0339(199703)16:3<221::aid-dc5>3.0.co;2-i
- 14. Ballo MS, Shin HJC. Sources of diagnostic error in the fine
needle aspiration diagnosis of Warthin’s tumor and clues to
a correct diagnosis. Diagnostic Cytopathology 1997; 17 (3):
230-234. doi: 10.1002/(sici)1097-0339(199709)17:3<230::aiddc12>
3.0.co;2-g
- 15. Chakrabarti I, Basu A, Ghosh N. Oncocytic lesion of parotid
gland: a dilemma for cytopathologists. Journal of Cytology
2012; 29 (1): 80-82. doi: 10.4103/0970-9371.93236
- 16. Lubin D, Song S, Zafar HM, Baloch Z. The key radiologic and
cytomorphologic features of oncocytic and oncocytoid lesions
of the salivary gland. Diagnostic Cytopathology 2019; 47 (6):
617-636. doi: 10.1002/dc.24175
- 17. Verma K, Kapila K. Salivary gland tumors with a prominent
oncocytic component: cytologic findings and differential
diagnosis of oncocytomas and Warthin’s tumor on fine
needle aspirates. Acta Cytologica 2003; 47 (2): 221-226. doi:
10.1159/000326508
- 18. Ashkavandi ZJ, Ashraf MJ. Increased mast cell counts in
benign and malignant salivary gland tumors. Journal of Dental
Research, Dental Clinics, Dental Prospects 2014; 8 (1): 15-20.
doi: 10.5681/joddd.2014.003
- 19. Kobayashi TK, Ueda M, Nishino T, Kushima R, Nakajima S et
al. Association of mast cells with Warthin’s tumor in fine needle
aspirates of the salivary gland. Acta Cytologica 1999; 43 (6):
1052-1058. doi: 10.1159/000331353
- 20. Bottles K, Löwhagen T, Miller TR. Mast cells in the aspiration
cytology differential diagnosis of adenolymphoma. Acta
Cytologica 1985; 29 (4): 513-515.
- 21. Song SJ, Shafique K, Wong LQ, LiVolsi VA, Montone KT et
al. The utility of the Milan System as a risk stratification tool
for salivary gland fine needle aspiration cytology specimens.
Cytopathology 2019; 30 (1): 91-98. doi: 10.1111/cyt.12642
- 22. Bellevicine C, Iaccarino A, Malapelle U, Troncone G. Warthin
tumor with signet-ring cell features as a pitfall in salivary gland
cytopathology. Acta Cytologica 2013; 57 (3): 309-312. doi:
10.1159/000348248