Muaz BELVİRANLI, Nilsel OKUDAN, Hakkı GÖKBEL, Aysel KIYICI, Mehmet ÖZ, Ayşe KUMAK

Cytokeratin 18 and h-FABP levels in intestinal ischemia–reperfusion injury: role of coenzyme Q10

The objective of this study was to investigate whether coenzyme Q10 (CoQ10) supplementation affects M30 and heart-type fatty acid binding protein (h-FABP) levels in intestinal ischemia-reperfusion injury-induced rats. Materials and methods: Thirty-two male Wistar rats were randomly divided into 4 groups. 1) Sham: Animals exposed to laparotomy without clamping the superior mesenteric artery (SMA); 2) ischemia-reperfusion (IR): rats exposed to laparotomy with occlusion of the SMA for 45 min, followed by 120 min of reperfusion period; 3) CoQ10 plus sham: 10 mg kg body weight-1 CoQ10 was administrated via intraperitoneal injection for 20 days, and thereafter animals were exposed to laparotomy without clamping the SMA; 4) CoQ10 plus IR: 10 mg kg body weight-1 CoQ10 was administrated via intraperitoneal injection for 20 days and thereafter animals were exposed to laparotomy with occlusion of the SMA for 45 min, followed by 120 min of reperfusion period. Blood samples were taken by cardiac puncture for the analysis of serum h-FABP and M30 levels. Results: There was no difference among the groups for serum h-FABP and M30 antigen levels. Conclusion: Neither intestinal ischemia–reperfusion injury nor CoQ10 supplementation affect serum h-FABP and M30 levels in rats.

Anahtar Kelimeler:

Key words: Ischemia–reperfusion injury, intestine, coenzyme Q10, M30, h-FABP

Cytokeratin 18 and h-FABP levels in intestinal ischemia–reperfusion injury: role of coenzyme Q10

Keywords:

Key words: Ischemia–reperfusion injury, intestine, coenzyme Q10, M30, h-FABP,

PDF

___

Pelsers MM, Hermens WT, Glatz JF. Fatty acid-binding proteins as plasma markers of tissue injury. Clin Chim Acta 2005; 352: 15–35.
Schaap FG, Binas B, Danneberg H, van der Vusse GJ, Glatz JF. Impaired long-chain fatty acid utilization by cardiac myocytes isolated from mice lacking the heart-type fatty acid binding protein gene. Circ Res 1999; 85: 329–37.
Nakata T, Hashimoto A, Hase M, Tsuchihashi K, Shimamoto K. Human heart-type fatty acid-binding protein as an early diagnostic and prognostic marker in acute coronary syndrome. Cardiology 2003; 99: 96–104.
Moll R, Franke WW, Schiller DL, Geiger B, Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982; 31: 11–24.
Linder S, Havelka AM, Ueno T, Shoshan MC. Determining tumor apoptosis and necrosis in patient serum using cytokeratin 18 as a biomarker. Cancer Lett 2004; 214: 1–9.
Yaman E, Coskun U, Sancak B, Buyukberber S, Ozturk B, Benekli M. Serum M30 levels are associated with survival in advanced gastric carcinoma patients. Int Immunopharmacol 2010; 10: 719–22.
Galluzzi L, Maiuri MC, Vitale I, Zischka H, Castedo M, Zitvogel L et al. Cell death modalities: classification and pathophysiological implications. Cell Death Differ 2007; 14: 1237–43.
Crane FL. Biochemical functions of coenzyme Q10. J Am Coll Nutr 2001; 20: 591–8.
Ahmed HH, Shousha WG, Hussien RM, Farrag ARH. Potential role of some nutraceuticals in the regression of Alzheimer’s disease in an experimental animal model. Turk J Med Sci 2011; 41: 455–66.
Juan YS, Chuang SM, Mannikarottu A, Huang CH, Li S, Schuler C et al. Coenzyme Q10 diminishes ischemia-reperfusion induced apoptosis and nerve injury in rabbit urinary bladder. Neurourol Urodyn 2009; 28: 339–42.
Bolcal C, Yildirim V, Doganci S, Sargin M, Aydin A, Eken A et al. Protective effects of antioxidant medications on limb ischemia reperfusion injury. J Surg Res 2007; 139: 274–9.
Ostrowski RP. Effect of coenzyme Q(10) on biochemical and morphological changes in experimental ischemia in the rat brain. Brain Res Bull 2000; 53: 399–407.
Portakal O, Inal-Erden M. Effects of pentoxifylline and coenzyme Q10 in hepatic ischemia/reperfusion injury. Clin Biochem 1999; 32: 461–6.
Horton JW, White DJ. Cardiac contractile injury after intestinal ischemia-reperfusion. Am J Physiol 1999; 261: 1164–70.
Stefanutti G, Vejchapipat P, Williams SR, Pierro A, Eaton S. Heart energy metabolism after intestinal ischaemia and reperfusion. J Pediatr Surg 2004; 39: 179–83.
Pierro A, Eaton S. Intestinal ischemia reperfusion injury and multisystem organ failure. Semin Pediatr Surg 2004; 13: 11–7.
Robinson JW, Mirkovitch V. The recovery of function and microcirculation in small intestinal loops following ischaemia. Gut 1972; 13: 784–9.
Gollin G, Marks C, Marks WH. Intestinal fatty acid binding protein in serum and urine reflects early ischemic injury to the small bowel. Surgery 1993; 113: 545–51.
Marks WH, Gollin G. Biochemical detection of small intestinal allograft rejection by elevated circulating levels of serum intestinal fatty acid binding protein. Surgery 1993; 114: 206–10.
Akbal E, Özbek M, Güneş F, Akyürek Ö, Üreten K, Delibaşı T. Serum heart type fatty acid binding protein levels in metabolic syndrome. Endocrine 2009; 36: 433–7.
Juan YS, Hydery T, Mannikarottu A, Kogan B, Schuler C, Leggett RE et al. Coenzyme Q10 protect against ischemia- reperfusion induced biochemical and functional changes in rabbit urinary bladder. Mol Cell Biochem 2008; 311: 73–80.
Ausch C, Buxhofer-Ausch V, Olszewski U, Hinterberger W, Ogris E, Schiessel R et al. Caspase-cleaved cytokeratin 18 fragment (M30) as marker of postoperative residual tumor load in colon cancer patients. Eur J Surg Oncol 2009; 35: 1164–8.
Ozturk B, Coskun U, Sancak B, Yaman E, Buyukberber S, Benekli M. Elevated serum levels of M30 and M65 in patients with locally advanced head and neck tumors. Int Immunopharmacol 2009; 9: 645–8.
Ueno T, Toi M, Bivén K, Bando H, Ogawa T, Linder S. Measurement of an apoptotic product in the sera of breast cancer patients. Eur J Cancer 2003; 39: 769–74.
Schumer M, Colombel MC, Sawczuk IS, Gobé G, Connor J, O’Toole KM et al. Morphologic, biochemical, and molecular evidence of apoptosis during the reperfusion phase after brief periods of renal ischemia. Am J Pathol 1992; 140: 831–8.
Topaloglu S, Abbasoglu O, Ayhan A, Sokmensuer C, Kilinc K. Antiapoptotic and protective effects of roscovitine on ischemia–reperfusion injury of the rat liver. Liver Int 2003; 23: 300–7.
Grootjans J, Lenaerts K, Derikx JP, Matthijsen RA, de Bruïne AP, van Bijnen AA et al. Human intestinal ischemia-reperfusion– induced inflammation characterized: experiences from a new translational model. Am J Pathol 2010; 176: 2283–91.
Ulukaya S, Ulukaya E, Alper I, Yilmaztepe-Oral A, Kilic M. Soluble cytokeratin 18 biomarkers may provide information on the type of cell death during early ischemia and reperfusion periods of liver transplantation. Clin Transplant 2010; 24: 848–54.