Handan ANKARALI, Mehmet YAŞAR, Murat OKTAY, Havva ERDEM, Ümran YILDIRIM, Ali Kemal UZUNLAR

AEG-1 expression in invasive ductal and lobular breast cancers and its relationship with prognostic parameters

The astrocyte-elevated gene-1 (AEG-1, also known as metadherin) is associated with various aspects of tumour malignancy; however, little knowledge is available related to the role of AEG-1 in ductal and lobular carcinomas. The aim of this study was to investigate the relationship of AEG-1 with the prognostic parameters in invasive ductal and lobular carcinomas. Materials and methods: This study was conducted on a total of 72 paraffin-embedded breast tumour samples. They consisted of 61 ductal and 11 lobular carcinomas. Breast tumour samples were stained for AEG-1. The prognostic parameters were compared with the results of AEG-1 stains. Results: We observed that more ductal carcinoma types than lobular carcinoma types resulted in high AEG-1 staining, whereas low AEG-1 staining occurred more in lobular carcinomas than in ductal carcinomas (P = 0.05). A significant negative correlation was also found between HER-2 (r = -0.30, P = 0.019) and AEG-1 (r = -0.804, P = 0.003) in lobular carcinomas. A significant relationship was found between increasing numbers of positive lymph nodes and AEG-1 in ductal carcinoma cases (P = 0.05). Conclusion: These results are consistent with previous reports of the role of AEG-1 in tumour progression. AEG-1 could be a useful marker for the development of new treatments and resistant hormonal therapy.

Anahtar Kelimeler:

Key words: Ductal carcinomas, lobular carcinomas, AEG-1, prognostic parameters

AEG-1 expression in invasive ductal and lobular breast cancers and its relationship with prognostic parameters

Keywords:

Key words: Ductal carcinomas, lobular carcinomas, AEG-1, prognostic parameters,

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Jemal A, Siegel R, Ward E, Hao Y, Xu J, Thun MJ. Cancer statistics 2009. CA Cancer J Clin 2009; 59: 225–49.
Berkowitz N, Gupta S, Silberman G. Estimates of the lifetime direct costs of treatment for metastatic breast cancer. Value Health 2000; 3: 23–30.
Rampaul RS, Pinder SE, Elaston CW, Ellis IO. Prognostic and predictive factors in primary breast cancer and their role in patient management; The Nottingham Breast Team. Eur J Surg Oncol 2001; 27: 229–38.
Mori I, Yang Q, Kakudo K. Predictive and prognostic markers for invasive breast cancer. Pathol Int 2002; 52: 186–94.
Gancberg D, Di Leo A, Cardoso F, Rouas G, Pedrocchi M, Paesmans M et al. Comparison of HER-2 status between primary breast cancer and corresponding distant metastatic sites. Ann Oncol 2002; 13: 1036–43.
Simmons C, Miller N, Geddie W, Gianfelice D, Oldfield M, Dranitsaris G et al. Does confirmatory tumor biopsy alter the management of breast cancer patients with distant metastases? Ann Oncol 2009; 20: 1499–504.
Tanner M, Jarvinen P, Isola J. Amplification of HER-2/neu and topoisomerase II alpha in primary and metastatic breast cancer. Cancer Res 2001; 61: 5345–8.
Zidan J, Dashkovsky I, Stayerman C, Basher W, Cozacov C, Hadary A. Comparison of HER-2 overexpression in primary breast cancer and metastatic sites and its effect on biological targeting therapy of metastatic disease. Br J Cancer 2005; 93: 552–
Brown DM, Ruoslahti E. Metadherin, a cell surface protein in breast tumors that mediates lung metastasis. Cancer Cell 2004; 5: 365–74.
Su ZZ, Kang DC, Chen Y, Pekarskaya O, Chao W, Volsky DJ et al. Identification and cloning of human astrocyte genes displaying elevated expression after infection with HIV-1 or exposure to HIV-1 envelope glycoprotein by rapid subtraction hybridization, RaSH. Oncogene 2002; 21: 3592–602.
Hu G, Chong RA, Yang Q, Wei Y, Blanco MA, Li F et al. MTDH activation by 8q22 genomic gain promotes chemoresistance and metastasis of poor prognosis breast cancer. Cancer Cell 2009; 15: 9–20.
Sarkar D, Emdad L, Lee SG, Yoo BK, Su ZZ, Fisher PB. Astrocyte elevated gene-1: far more than just a gene regulated in astrocytes. Cancer Res 2009; 69: 8529–35.
Hu G, Wei Y, Kang Y. The multifaceted role of MTDH/AEG-1 in cancer progression. Clin Cancer Res 2009; 15: 5615–20. Emdad L, Lee SG, Su ZZ, Jeon HY, Boukerche H, Sarkar D et al. Astrocyte elevated gene-1 (AEG-1) functions as an oncogene and regulates angiogenesis. Proc Natl Acad Sci USA 2009; 106: 21300–5.
Li J, Yang L, Song L, Xiong H, Wang L, Yan X et al. Astrocyte elevated gene-1 is a proliferation promoter in breast cancer via suppressing transcriptional factor FOXO1. Oncogene 2009; 28: 3188–96.
Su P, Zhang Q, Yang Q. Immunohistochemical analysis of metadherin in proliferative and cancerous breast tissue. Diagn Pathol 2010; 5: 38.
Arpino G, Weiss H, Lee AV, Schiff R, De Placido S, Osborne CK et al. Estrogen receptor-positive, progesterone receptornegative breast cancer: association with growth factor receptor expression and tamoxifen resistance. J Natl Cancer Inst 2005; 97: 1254–61.
Piccart-Gebhart MJ, Procter M, Leyland-Jones B, Goldhirsch A, Untch M, Smith I et al. Trastuzumab after adjuvant chemotherapy in HER2- positive breast cancer. N Engl J Med 2005; 353: 1659–72.