Correlations of Serum IL-6 Levels and Prolidase Activity Between Bone Turnover Markers and Bone Mineral Density in Postmenopausal Women With and Without Osteoporosis
Aim: The purpose of this study was to measure serum prolidase activity and interleukin-2 and 6 (IL-2, IL-6) levels and to investigate the relationship of these parameters with bone mineral density (BMD) and bone turnover markers in postmenopausal women with and without osteoporosis. Materials and Methods: 59 postmenopausal women were included in this study (21 women with osteoporosis and 38 without). Serum IL-2 and IL-6 levels, prolidase activity, osteocalcin (OSC), calcium (Ca), alkaline phosphatase (ALP), urinary deoxypyridinoline (DPD) and BMD were measured in postmenopausal women with and without osteoporosis. Results: Serum prolidase activity was slightly higher in postmenopausal nonosteoporotic women than in postmenopausal osteoporotic women, but the difference did not reach a statistical significance. Mean values of OSC and IL-6 levels were significantly higher in postmenopausal osteoporotic than in nonosteoporotic women (P < 0.05). There were significant correlations between OSC with IL-6 (r = 0.545, p = 0.01) and urinary DPD (r = -0.513, p = 0.01) in postmenopausal osteoporotic women. Conclusions: Serum prolidase activity in postmenopausal osteoporotic women was not correlated with the bone turnover markers and BMD. Elevated levels of IL-6 in postmenopausal osteoporosis might have an important role in the pathogenesis of postmenopausal osteoporosis.
Correlations of Serum IL-6 Levels and Prolidase Activity Between Bone Turnover Markers and Bone Mineral Density in Postmenopausal Women With and Without Osteoporosis
Aim: The purpose of this study was to measure serum prolidase activity and interleukin-2 and 6 (IL-2, IL-6) levels and to investigate the relationship of these parameters with bone mineral density (BMD) and bone turnover markers in postmenopausal women with and without osteoporosis. Materials and Methods: 59 postmenopausal women were included in this study (21 women with osteoporosis and 38 without). Serum IL-2 and IL-6 levels, prolidase activity, osteocalcin (OSC), calcium (Ca), alkaline phosphatase (ALP), urinary deoxypyridinoline (DPD) and BMD were measured in postmenopausal women with and without osteoporosis. Results: Serum prolidase activity was slightly higher in postmenopausal nonosteoporotic women than in postmenopausal osteoporotic women, but the difference did not reach a statistical significance. Mean values of OSC and IL-6 levels were significantly higher in postmenopausal osteoporotic than in nonosteoporotic women (P < 0.05). There were significant correlations between OSC with IL-6 (r = 0.545, p = 0.01) and urinary DPD (r = -0.513, p = 0.01) in postmenopausal osteoporotic women. Conclusions: Serum prolidase activity in postmenopausal osteoporotic women was not correlated with the bone turnover markers and BMD. Elevated levels of IL-6 in postmenopausal osteoporosis might have an important role in the pathogenesis of postmenopausal osteoporosis.
___
- Myara I, Myara A, Mangeot M, Fabre M, Charpentier C, Lemon- nier A. Plasma prolidase activity: a possible index of collagen catabolism in chronic liver disease. Clin Chem 1984; 30: 211- 215.
- Miltyk W, Annchim T, Wolcyynski S, Pakla J. Estrogen-dependent regulation of prolidase activity in breast cancer MCF-7 cells. Gynecol Endocrinol 1999; 13: 166-174.
- Chiu KM, Arnaud CD, Ju J, Mayes D, Bacchetti P, Weitz S et al. Correlation of estradiol, parathyroid hormone, interleukin-6 and soluble interleukin-6 receptor during the normal menstrual cycle. Bone 2000; 26: 79-85.
- Chaudhary LR, Spelsbert TC, Rggs BL. Production of various cytokines by normal human osteoblast-like cells in response to interleukin-1 beta and tumor necrosis factor alpha: lack of regu- lation by 17-beta estradiol. Endocrinology 1992; 130: 2528- 2534.
- Rickard D, Russel G, Gowen M. Oestradiol inhibits the release of tumor necrosis factor but not interleukin-6 from adult human osteoblasts in vitro. Osteoporos Int 1992; 2: 94-102.
- Rifas L, Kenney JS, Marcelli M, Pacifici R, Cheng SL, Dawson LL et al. Production of interleukin-6 in human osteoblasts and human bone marrow stromal cells: evidence that induction by interleukin- 1 and tumor necrosis factor alpha is not regulated by ovarian steroids. Endocrinology 1995; 136: 4056-4067.
- McKane WR, Khosla S, Peterson JM, Egan K, Riggs BL. Circulat- ing levels of cytokines that modulate bone resorption: effects of age and menopause in women. J Bone Miner Res 1994; 9: 1313- 1318.
- Rachon D, Mysliwska J, Suchecka-Rachon K, Wieckiewicz J, Mys- liwski A. Effect of oestrogen deprivation on interleukin-6 produc- tion by peripheral blood mononuclear cells of postmenopausal women. J Endocrinol 2002; 172: 387-395.
- Straub RH, Hense HW, Andus T, Scholmerich J, Riegger GA, Schunkert H. Hormone replacement therapy and interrelation between serum interleukin-6 and body mass index in post- menopausal women: a population based study. J Clin Endocrinol Metab 2000; 85: 1340-1344.
- Scheidt-Nave C, Bismar H, Leidig-Bruckner G, Woitge H, Seibel MJ, Ziegler R et al. Serum interleukin-6 is a major predictor of bone loss in women specific to the first decade past menopause. J Clin Endocrinol Metab 2001; 86: 2032-2042.
- Pacifici R. Estrogen, cytokines and pathogenesis of post- menopausal osteoporosis. J Bone Miner Res 1996; 11: 1043- 1051.
- Gür A, Denli A, Cevik R, Nas K, Karakoc M, Sarac AJ. The effects of alendronate and calcitonin on cytokines in postmenopausal osteoporosis. A 6-month randomized and controlled study. Yon- sei Med J 2003; 44: 99-109.
- Verit FF, Geyikli I, Yazgan P, Celik A. Correlations of serum pro- lidase activity between bone turnover markers and mineral densi- ty in postmenopausal osteoporosis. Arch Gynecol Obstet 2006; 274 (3): 133-137.
- Sumino H, Ichikawa S, Abe M, Endo Y, Nakajima Y, Minegishi T et al. Effects of aging and postmenopausal hypoestrogenism on skin elasticity and bone mineral density in Japaenese women. Endocr J 2004; 51: 159-164.
- Surazynski A, Jarzabek K, Haczynski J, Laudanski P, Palka J, Wolczynski S. Differential effects of estradiol and raloxifene on collagen biosynthesis in cultured human skin fibroblasts. Int J Mol Med 2003; 12: 803-809.
- Pacifici R. Editorial. Cytokines, estrogen and postmenopausal osteoporosis - the second decade. Endocrinology 1998; 139: 2659-2561.
- Horowitz MC. Cytokines and estrogen in bone: antiosteoporotic effects. Science 1993; 260: 626-627.
- Rachon D, Mysliwska J, Suchecka-Rachon K, Semetkowska- Jurkiewicz B, Zorena K, Lysiak-Szydlowska W. Serum interleukin- 6 levels and bone mineral density at the femoral neck in post- menopausal women with type 1 diabetes. Diabetic Med 2003; 20: 475-480.
- Kim JG, Ku SY, Lim KS, Jee BC, Suh CS, Kim SH et al. Cytokine production by whole blood cells: relationship to interleukin gene polymorphism and bone mass. J Korean Med Sci 2005; 20: 1017-1022.
- Kania DM, Brinkley N, Checovich M, Havighurst T, Schilling M, Ershler WB. Elevated plasma levels of interleukin-6 in post- menopausal women do not correlate with bone density. J Am Geri Soc 1995; 43: 236-239.