Halise AKÇA, Cüneyt TAYMAN, Emin METE, Ferhat ÇATAL, Nurdan URAS, Fatih ÖZKARAGÖZ, Mustafa ULUKANLIGİL

Comparison of human milk, cow’s milk and infant formulas for their antifungal effects against environmental fungi

Amaç: Annesütü ile beslenme genel olarak bebek beslenmesinin en uygun şekli olarak kabul edilir. Sunulan çalışmanın amacı anne sütünün antifungal etkisinin araştırılması ve inek sütü ile formül mamalarla karşılaştırılmasıdır. Yöntem ve Gereç: Seksen adet Sabouraud agar besiyeri alınarak 8 gruba bölündü. Tüm petri kutuları kapakları açık durumda 15 dakika bekletildikten sonra kapatılarak inkubasyona bırakıldı. İlk grup çevre havasının fungal florasını tesbit etmek için kullanıldı. İkinci gruptaki besiyerlerine anne sütü uygulandı. Üçüncü gruba 0.9% NaCl solüsyonu uygulandı. Dördüncü gruba inek sütü uygulandı. Beş, 6, 7 ve 8. gruplara sırasıyla probiyotik formüla, prebiyotik formula, pro-prebiyotik formula ve standart formula uygulandı. İstatistiksel değerlendirmeler için SPSS programı uygulandı. Bulgular: Anne sütü uygulanan besiyerlerinde üreyen fungal koloni sayıları diğer gruplardan 7.grup hariç anlamlı derecede düşüktü (grup 1, 3, 4, 5, 6, 7 ve 8) (sırasıyla; P < 0.001, P < 0.001, P < 0.001, P < 0.001, P < 0.05, P > 0.05, P < 0.0001). Sonuç: Anne sütünün in vitro antifungal etkisi inek sütü ve formül mamalardan anlamlı derecede üstündür. Anne sütü çocuklar için yalnızca en iyi besin maddesi olmakla kalmaz, aynı zamanda antifungal etkisi ile onları fungal infeksiyonlardan ve fungal allerjilerden korur. Prebiyotik ve pre-probiyotik formulalar da antifungal etkiye sahiptir

Anne sütünün antifungal etkisinin inek sütü ve formül mamalarla karşılaştırılması

Aim: Breast-feeding is generally accepted as the optimal method of feeding infants. The purpose of the present study was to investigate and compare the antifungal effect of human milk with infant formula and cow’s milk. Materials and Methods: Eighty Sabouraud agar petri dishes were separated into eight groups. All petri dish lids were closed and incubated, after 15 minutes uncovered. The first group was used to detect the fungal flora of the environmental air. The second group was rubbed with human milk. The third group was rubbed with 0.9% NaCl solution. Fourth group was rubbed with cow’s milk. Fifth, 6, 7 and 8. groups were rubbed with probiotic formula, prebiotic formula, pro-prebiotic formula and standard infant formula respectively. All statistical analyses were performed using the SPSS. Results: The number of fungal colonies grown in human milk–rubbed dishes was significantly less than the other groups except group 7 (groups 1, 3, 4, 5, 6, 7 and 8) (P < 0.001, P < 0.001, P < 0.001, P < 0.001, P < 0.05, P > 0.05, P < 0.0001, respectively). Conclusions: In vitro antifungal effect of human milk is significantly superior than cow’s milk and formulas tested. Human milk is not only the best food for infants but also it protects infants from fungal infections and fungal allergies by means of antifungal effect. Prebiotic and pre-probiotic formula also have antifungal effect.

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