Comparative study of virulence factors among ESβL-producing and nonproducing Pseudomonas aeruginosa clinical isolates

b-Lactamase production is considered one of the most important resistance mechanisms among virulent Pseudomonas aeruginosa isolates. The aim of this study was to compare the production and antimicrobial resistance patterns of some virulence factors in extended spectrum b-lactamase (ESbL)-producing and nonproducing P. aeruginosa clinical isolates. Materials and methods: Out of 183 different clinical specimens, 104 Pseudomonas aeruginosa isolates were recovered. The isolates were screened for ESbL production using the double disk diffusion test and phenotypic confirmatory disk diffusion test. All isolates were tested for susceptibility to 25 antimicrobials, as well as for expression of various virulence factors including pigment, hemolysin, gelatinase, protease, lipase, rhamnolipids, biofilm, and cell surface hydrophobicity. The results of ESbL producers and nonproducers were statistically compared. Results: All isolates showed a high frequency of multiple resistance to at least 14 and up to 25 of the tested antimicrobials. Nevertheless, most virulence factors were produced at higher rates in ESbL-producing than in ESbL-nonproducing Pseudomonas aeruginosa isolates. Conclusion: The results of this study suggest a correlation between ESbL phenotype and the production of some factors that are reported to be involved in the virulence of P. aeruginosa.

Comparative study of virulence factors among ESβL-producing and nonproducing Pseudomonas aeruginosa clinical isolates

b-Lactamase production is considered one of the most important resistance mechanisms among virulent Pseudomonas aeruginosa isolates. The aim of this study was to compare the production and antimicrobial resistance patterns of some virulence factors in extended spectrum b-lactamase (ESbL)-producing and nonproducing P. aeruginosa clinical isolates. Materials and methods: Out of 183 different clinical specimens, 104 Pseudomonas aeruginosa isolates were recovered. The isolates were screened for ESbL production using the double disk diffusion test and phenotypic confirmatory disk diffusion test. All isolates were tested for susceptibility to 25 antimicrobials, as well as for expression of various virulence factors including pigment, hemolysin, gelatinase, protease, lipase, rhamnolipids, biofilm, and cell surface hydrophobicity. The results of ESbL producers and nonproducers were statistically compared. Results: All isolates showed a high frequency of multiple resistance to at least 14 and up to 25 of the tested antimicrobials. Nevertheless, most virulence factors were produced at higher rates in ESbL-producing than in ESbL-nonproducing Pseudomonas aeruginosa isolates. Conclusion: The results of this study suggest a correlation between ESbL phenotype and the production of some factors that are reported to be involved in the virulence of P. aeruginosa.

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  • Ullah A, Durrani R, Ullah I, Rafiq M. Antibiotic resistance profile of clinical gram negative bacteria. J Biologic Food Sci Res 2012; 1: 23–25.
  • Morales-Espinosa R, Soberon-Chavez G, Delgado-Sapien G, Sandner-Miranda L. Genetic and phenotypic characterization of a Pseudomonas aeruginosa population with high frequency of genomic islands. PLOS ONE 2012; 7: e37459.
  • Kindrachuk KN. Activity of and resistance to cationic antimicrobial agents in Pseudomonas aeruginosa. J Antimicrob Chemother 2010; 29: 427–433.
  • Timani RJ. Molecular typing and biofilm characterization of Pseudomonas aeruginosa isolated from clinical samples in Lebanon. MSc, Lebanese American University, Beirut, Lebanon, 2012.
  • Kapoor R, Wadman MW, Dohm MT, Czyzewski AM, Spormann AM, Barron AE. Antimicrobial peptoids are effective against Pseudomonas aeruginosa biofilms. Antimicrob Agents Chemother 2011; 55: 3054–3057.
  • Mansouri S, Norouzi F, Moradi M, Nakhaae N. Comparison of virulence factors among clinical isolates of Pseudomonas aeruginosa producing and non-producing extended spectrum beta-lactamases. Curr Res Bacteriol 2011; 4: 85–93.
  • Kalaivani R. Antibiotic resistance mechanisms in clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital. MD thesis, Pondicherry Institute of Medical Sciences, Puducherry, India; 2011.
  • Taslima Y. Prevalence of ESβL among E. coli and Klebsiella Spp. in a tertiary care hospital and molecular detection of important ESβL producing genes by multiplex PCR. PhD, Mymensingh Medical College, Mymensingh, Bangladesh; 2012.
  • Krieg NR, Holt JG, editors. Bergey’s Manual for Systematic Bacteriology, Vol. 2. Baltimore, MD, USA: Williams and Wilkins; 2001.
  • Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing, 20th Informational Supplement. Wayne, PA, USA: Clinical and Laboratory Standards Institute; 2010.
  • Brooks E, Melnick JL, Adelberg EA. Medical Microbiology. 24th ed. New York, NY, USA: McGraw-Hill; 2007.
  • Pavlov D, De Wet CME, Grabow WOK, Ehlers MM. Potentially pathogenic features of heterotrophic plate count bacteria isolated from treated and untreated drinking water. Int J Food Microbiol 2004; 92: 275–287.
  • MacFaddin JF. Biochemical Tests for Identification of Medical Bacteria. 2nded. Baltimore, MD, USA: Williams and Wilkins; 1980.
  • Madigan MT, Martinko JM, Parker J. Brock Biology of Microorganisms. 9th ed. Upper Saddle River, NJ, USA: Prentice Hall; 1999.
  • Zulianello L, Canard C, Kohler T, Caille D, Lacroix JS, Meda P. Rhamnolipids are virulence factors that promote early infiltration of primary human airway epithelia by Pseudomonas aeruginosa. Infect Immun 2006; 74: 3134–3147.
  • Christensen CD, Simpson WA, Younger JJ. Adherence of coagulase negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. J Clin Microbiol 1985; 22: 996–1006.
  • Rosenberg M, Gutnick D. Adherence of bacteria to hydrocarbons: a simple method for measuring cell-surface hydrophobicity. FEMS Microbiol Lett 1980; 9: 29–33.
  • Kader AA, Angamuthu KK, Kamath KA, Zaman MN. Modified double-disc test for detection of extended-spectrum beta-lactamases in Escherichia coli and Klebsiella pneumoniae. Br J Biomed Sci 2006; 63: 51–54.
  • Aouf A. Bacteriological and molecular study of Pseudomonas aeruginosa producing elastase. MSc, Al-Kufa University, Najaf, Iraq; 2001.
  • Gad GF, El-Domany RA, Zaki S, Ashour HM. Characterization of Pseudomonas aeruginosa isolated from clinical and environmental samples in Minia: prevalence, antibiogram and resistance mechanisms. J Antimicrob Chemother 2007; 60: 1010–1017.
  • Asaad AM, Al-Yousef S, Al-gharawi A. Metallo-β-lactamases producing Pseudomonas aeruginosa clinical isolates from a military hospital, Saudi Arabia. Egypt J Med Microbiol 2009; 18: 81–88.
  • Murray PR, Rosenthal KS, Pfaller MA. Medical Microbiology. 5th ed. Philadelphia, PA, USA: Elsevier Mosby; 2005.
  • Casadevall A, Pirofski L. Virulence factors and their mechanisms of action. Water Health J 2009; 7: S1–S18.
  • Davey ME, Caiazza NC, O’Toole GA. Rhamnolipid surfactant production affects biofilm architecture in Pseudomonas aeruginosa PAO1. J Bacteriol 2003; 185: 1027–1036.
  • Norouzi F, Mansouri S, Moradi M, Razavi M. Comparison of cell surface hydrophobicity and biofilm formation among ESβL- and non-ESβL-producing Pseudomonas aeruginosa clinical isolates. Afr J Microbiol Res 2010; 4: 1143–1147.
  • Srinivasa Rao R, Uma Karthika R, Singh SP, Prashanth K. Correlation between biofilm production and multiple resistance in imipenem resistant clinical isolates of Acinetobacter baumannii. Indian J Med Microbiol 2008; 26: 333–337.
  • Fazlul M, Zaini MZ, Rashid MA, Nazmul MHM. Antibiotic susceptibility profiles of clinical isolates of Pseudomonas aeruginosa from Selayang Hospital. Malay Biomed Res 2011; 22: 263–266.
  • Radha K, Mahima R, Ramanathan G, Thangapandian V. Survey on drug resistant pattern of clinical isolates and effect of plant extract on the drug resistant pattern. ISCA J Biolog Sci 2012; 1: 14–19.
  • Finlayson EA, Brown PD. Comparison of antibiotic resistance and virulence factors in pigmented and non-pigmented Pseudomonas aeruginosa. West Indian Med J 2011; 60: 24-32.
  • Eftekhar F, Hosseinkhan N, Asgharzadeh A, Tabatabaii A. Genetic profiling of Pseudomonas aeruginosa isolates from Iranian patients with cystic fibrosis using RAPD-PCR and PFGE. Iranian J Basic Med Sci 2009; 12: 126–132.
  • Sharma J, Sharma M, Roy P. Detection of TEM and SHV genes in Escherichia coli and Klebsiella pneumoniae isolates in a tertiary care hospital from India. Indian J Med Res 2010; 132: 332–336.
  • Yusha’u MM, Kumurya AS, Suleiman L. Prevalence of extended spectrum β-lactamases among Enterobacteriaceae in Murtala Mohammed specialist hospital, Kano, Nigeria. Bayero J Pure Appl Sci 2010; 3:169–172.
  • Kiratisin, P, Apisarnthanarak A, Laesripa C, Saifon P. Molecular characterization and epidemiology of extended- spectrum β-lactamase producing Escherichia coli and Klebsiella pneumoniae isolates causing health care-associated infections in Thailand, where the CTX-M family is endemic. Antimicrob Agents Chemother 2008; 52: 2818–2824.
  • Akhi MT, Khalili Y, Ghottaslou R, Aghazadeh M, Haghi MSB, Yousefi S. Prevalence of PER-1-type extended-spectrum beta- lactamases in clinical strains of Pseudomonas aeruginosa isolated from Tabriz, Iran. Iranian J Basic Med Sci 2012; 15: 678–682.
  • Johnson JR, Kushowski MA, Owens K, Gajewski A, Winokur PL. Phylogenetic origin and virulence genotype in relation to resistance to fluoroquinolones and/or extended spectrum cephalosporins and cephamycins among Escherichia coli isolates from animals and humans. J Infect Dis 2003; 188: 759–768.
Turkish Journal of Medical Sciences-Cover
  • ISSN: 1300-0144
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
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