Ali ATAŞ, Fuat DİLMEÇ

8381

Characterization of the human GYS2 gene and its product using bioinformatic tools

Aim: The GYS2 gene, which encodes for glycogen synthase 2 (liver) (GS), is an enzyme responsible for the synthesis of 1,4-linked glucose chains in glycogen. The present study aimed to investigate the homology, conserved domain, and promoter and expression profiles of the human GYS2 gene among various vertebrate species using bioinformatic tools. Materials and Methods: We analyzed the homology with NCBI blast, the conserved domain with EBI ClustalW and Mega4, the promoter with Genomatix, and the expression profiles with DigiNorthern software. Results: GS proteins and their conserved domains (Glycogen-syn) were more conserved in all the organisms investigated. There was 1 fully conserved domain (Glycogen-syn) and several truncated sub-domains. Comparative screening of the promoters showed that GYS2 genes did not have any common conserved transcription factor binding sites. Conclusions: This study shows that the GS molecules in various species, except Ornithorhynchus anatinus and Danio rerio, were well conserved throughout evolution.

Anahtar Kelimeler:

Glycogen synthase, genomics, evolution, promoter, expression

Characterization of the human GYS2 gene and its product using bioinformatic tools

Aim: The GYS2 gene, which encodes for glycogen synthase 2 (liver) (GS), is an enzyme responsible for the synthesis of 1,4-linked glucose chains in glycogen. The present study aimed to investigate the homology, conserved domain, and promoter and expression profiles of the human GYS2 gene among various vertebrate species using bioinformatic tools. Materials and Methods: We analyzed the homology with NCBI blast, the conserved domain with EBI ClustalW and Mega4, the promoter with Genomatix, and the expression profiles with DigiNorthern software. Results: GS proteins and their conserved domains (Glycogen-syn) were more conserved in all the organisms investigated. There was 1 fully conserved domain (Glycogen-syn) and several truncated sub-domains. Comparative screening of the promoters showed that GYS2 genes did not have any common conserved transcription factor binding sites. Conclusions: This study shows that the GS molecules in various species, except Ornithorhynchus anatinus and Danio rerio, were well conserved throughout evolution.

Keywords:

Glycogen synthase, genomics, evolution, promoter, expression,

Turkish Journal of Medical Sciences-Cover

ISSN: 1300-0144
Yayın Aralığı: 6
Yayıncı: TÜBİTAK

Arşiv

Sayıdaki Diğer Makaleler

Lack of association between TLR4 polymorphism and severe gram-negative bacterial infection in neonates

İlhan TEZCAN, Leman YEL, Şule YİĞİT, Gülsevin TEKİNALP, Lütfiye MESCİ, Özden SANAL, Turul Tuba ÖZGÜR, Fügen ERSOY

HIV/AIDS knowledge among a group of youngsters under social protection in Turkey

Veli DUYAN, Çamur Gülsüm DUYAN

Survival in esophageal cancer patients with hematogenous distant organ metastases

Ömer YILMAZ, Atila EROĞLU, Atila TÜRKYILMAZ, Yener AYDIN, Nurettin KARAOĞLANOĞLU

Disseminated herpes zoster infection in a patient with lymphoma

Semra PAYDAŞ, Süheyla KÖMÜR, Aslihan CANDEVİR, Hasan Salih Zeki AKSU, Behice KURTARAN

Acute effect of speed exercise on nitric oxide (NO) level of footballers

Comparison of light emitting diode phototherapy and double standard conventional phototherapy for nonhemolytic neonatal hyperbilirubinemia

M. Mansur TATLI, Alparslan TONBUL, Nurdan URAŞ, Müsemma KARABEL, güzide DOĞAN, Ahmet KARADAĞ

A rare cause of neonatal hypocalcemia: malignant infantile osteopetrosis

Kenan KOCABAY, Hakan UZUN, Bünyamin DİKİCİ, Yılmaz Ebru KESKİN, Ayhan ERDEM, Dursun Ali ŞENSES

Fibromyalgia syndrome in patients with chronic sinusitis

M. Akif ÖZTÜRK, Adnan ÜNAL, Mesut S. TEZER, Zeynep ÖZBALKAN, Güçlü Damla GÜVEN, Yaşar KARAARSLAN

Isolated fungal sinusitis of the sphenoid sinus

Halit AKMANSU, Muharrem DAĞLI, Özge HAN, Adil ERYILMAZ, Ayşe İRİZ, Bengi ARSLAN

The relation between gleason score, and nuclear size and shape factors in prostatic adenocarcinoma

Şükrü Oğuz ÖZDAMAR, Doğan Banu GÜN, Sibel BEKTAŞ, Burak BAHADIR, Gürkan KERTİŞ