An investigation of genes coding fi bronectin binding proteins in Staphylococcus aureus isolated from carriers aged between 6 and 14 years

Amaç: Staphylococcus aureus’un burun taşıyıcılığı değişik topluluklarda % 18 ile % 50 oranında bildirilmektedir ve bu durum invazif infeksiyonlar için risk oluşturmaktadır. Bu çalışmanın amacı, S. aureus’un insan epiteline bağlanmasını sağlayan fi bronectin bağlayan proteinlerle ilişkili genleri araştırmaktı. Yöntem ve gereç: Çocukların burun sürüntülerinden üreyen 50 köken çalışmaya alındı. Örnekler mannitol salt agara ekildi ve 37 °C’de 24-48 saatlik inkübasyondan sonra kökenler biyokimyasal özelliklerine göre tanımlandı. Fibronectin bağlayan protein A ve B’yi kodlayan fnbA ve fnbB genleri PCR yöntemiyle araştırıldı. Kontrol kökeni olarak S. aureus NCTC8325 kullanıldı. Bulgular: Bütün kökenler kültür özellikleri ve tüp koagülaz testinin pozitifl iği ile S. aureus olarak tanımlandı. Elli S. aureus kökeninin 14’ünde (% 28) fnbA ve 5’inde (% 10) fnbB geni saptandı. Sonuç: Çalışma grubumuzda fnbA ve fnbB saptanma oranı, burun taşıyıcılarında yapılmış önceki çalışmalardan düşüktür. Bu düşük oran konusunda çalışma grubundaki taşıyıcıların yaşlarının küçük olmasının yanında coğrafi k değişikliklerin de etkili olabileceği yorumu yapılabilir.

Altı ondört yaş arasındaki taşıyıcılardan soyutlanan Staphylococcus aureus kökenlerinde fi bronectin bağlayan proteinleri kodlayan genlerin araştırılması

Aim: Nasal carriage rates for Staphylococcus aureus have been reported to vary between 18% and 50% in diff erent populations and it represents a risk factor for invasive infections. Th e aim of the present study was to investigate the presence of genes associated with fi bronectin binding proteins mediating the adhesion of S. aureus to human epithelial cells. Materials and methods: Fift y strains isolated from nasal swab specimens of children were included. Specimens were inoculated on mannitol salt agar plates and aft er 24-48 h of incubation at 37 °C the isolates were identifi ed according to their biochemical properties. Th e presence of fnbA and fnbB genes encoding fi bronectin binding protein A and B was investigated via PCR. S. aureus NCTC8325 was used as the reference strain. Results: All isolates were identifi ed as S. aureus according to their cultural properties and the positive tube coagulase test results. Of the 50 S. aureus strains, 14 (28%) were positive for fnbA and 5 (10%) for fnbB. Conclusion: Th e presence of fnbA and fnbB in our study population was lower than in previous studies performed in nasal carriers. We concluded that this diff erence might have resulted from the lower age of the study population and geographical diversities.

PDF

___

1. Nashev D, Toshkava K, Salasia SIO, Hassan AA, Lammler C, Zschöck M. Distribution of virulence genes of Staphylococcus aureus isolated from stable nasal carriers. FEMS Microbiol Lett 2004; 233: 45-52.
2. van Belkum A. Staphylococcal colonization and infection: Homeostasis versus disbalance of human (innate) immunity and bacteria virulence Curr Opin Infect Dis 2006; 19: 339-44.
3. Erdenizmenli M, Yapar N, Serin-Senger S, Özdemir S, Yüce A. Investigation of colonization with methicillin-resistant and methicillin-susceptible Staphylococcus aureus in an outpatient population in Turkey. Jpn J Infect Dis 2004; 57: 172-5.
4. Choi CS, Yin CS, Bakar AA, Sakewi Z, Naing NN, Jamal F et al. Nasal carriage of Staphylococcus aureus among healthy adults. J Microbiol Immunol Infect 2006; 39: 458-64.
5. Wertheim HFL, Melles DC, Vos MC, van Leeuwen W, van Belkum A, Verbrugh H et al. Th e role of nasal carriage in Staphylococcus aureus infections. Lancet Infect Dis 2005; 5:751-62.
6. Burke FM, McCormack N, Rindi S, Speziale P, Foster TJ. Fibronectin-binding protein B variation in Staphylococcus aureus. BMC Microbiology 2010; 10: 160.
7. Elasri MO, Th omas JR, Skinner RA, Blevins JS, Beenken KE, Nelson Cl et al. Staphylococcus aureus collagen adhesin contributes to the pathogenesis of osteomyelitis. Bone 2002; 30:275-80.
8. Martinez-Aguilar G, Avalos-Mishaan A, Hulten K, Hammerman W, Mason Jr EO, Kaplan SL. Communityacquired, methicillin-resistant and methicillin-susceptible Staphylococcus aureus musculoskeletal infections in children. Pediatr Infect Dis J 2004; 23: 701-6.
9. Arciola CR, Campoccia D, Gamberini S, Baldassari L, Montanaro L. Prevalence of cna, fnbA and fnbB adhesin genes among Staphylococcus aureus isolates from orthopedic infections associated to diff erent types of implant. FEMS Microbiology Letters 2005; 246: 81-6.
10. Unal S, Hoskins JA, Flokowitsch JE, Preston WE, Skatrud PL. Detection of methicillin-resistant staphylococci by using the polymerase chain reaction. J Clin Microbiol 1992; 30: 1685-91.
11. Avkan-Oğuz V, Dodanlı S, Yıldırım İ, Öztürk N, Sarıgüzel N, Seber E. Metisiline dirençli Staphylococcus aureus (MRSA) prevalansının farklı yöntemlerle araştırılması. Flora 2001; 6:178-83.
12. Strommenger B, Kettlitz C, Werner G, Witte W. Multiplex PCR assay for simultaneous detection of nine clinically relevant antibiotic resistance genes in Staphylococcus aureus. J Clin Microbiol 2003; 41: 4089-94.
13. Cho SH, Strickland I, Boguniewicz M, Leung DYM. Fibronectin and fi brinogen contribute to the enhanced binding of Staphylococcus aureus to atopic skin. J Allergy Clin Immunol 2001; 108: 269-74.
14. Mishaan AMA, Mason EO, Martinez-Aguilar G, Hammerman W, Propst JJ, Lupski JR et al. Emergence of a predominant clone of community-acquired Staphylococcus aureus among children in Houston, Texas. Pediatr Infect Dis J 2005; 24: 201-6.
15. Zmantar T, Chaieb K, Makni H, Miladi H, Abdallah FB, Mahdouani K et al. Detection by PCR of adhesins genes and slime production in clinical Staphylococcus aureus. J Basic Microbiol 2008; 48: 308-14.
16. Gonano M, Hein H, Zangerl P, Rammelmayr A, Wagner M. Phenotypic and molecular characterization of Staphylococcus aureus strains of veterinary, diary and human origin. Epidemiol Infect 2008; 23:1-12.
17. Scarpa M, Piccini R, Brun P, Grillo A, Palu G, Mengoli C et al. Relationship between virulence factor genes in bovine Staphylococcus aureus subclinical mastitis isolates and binding to anti-adhesin antibodies. J Dairy Res 2010; 77: 159-67.