Alterations in serum levels of copper, zinc, and selenium among children with sickle cell anemia
Alterations in serum levels of copper, zinc, and selenium among children with sickle cell anemia
Background/aim: Oxidative stress contributes to pathophysiological dysfunction in sickle cell anemia (SCA). Copper (Cu) is aprooxidant, whereas zinc (Zn) and selenium (Se) are antioxidant trace elements. This study investigates the serum levels of Cu, Zn, andSe among children with SCA.Materials and methods: This cross-sectional study was performed at King Khalid University Hospital, Riyadh. Thirty-three childrenwith SCA in steady state and 33 age- and sex-matched normal healthy children were included in the study. Cu, Zn, and Se levels weremeasured by inductively coupled plasma-mass spectrometry (ICP-MS) instrument.Results: The median serum Cu levels among SCA patients (1.3 μg/mL) were higher than those of the controls (0.88 μg/mL; P < 0.0001).Zn (0.61 μg/mL) and Se (74 ng/mL) levels among SCA patients, however, were significantly lower than those of the controls (0.94 μg/mL; P < 0.0001) and (91.2 ng/mL; P < 0.0001), respectively. The Cu/Zn ratio among SCA patients (1.92) was higher than that of thecontrols (0.98).Conclusion: Decreased blood levels of antioxidant trace elements may contribute to the pathophysiology in SCA by promoting oxidativestress. The monitoring of trace element levels in SCA appears to be vital for decreasing morbidity associated with the disorder.
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- 1. Thornburg CD, Kalpatthi RV, Barredo JC, Brown RC, Sarnaik
S et al. The pediatric hydroxyurea phase III clinical trial (BABY
HUG): challenges of study design. Pediatric Blood & Cancer
2010; 54: 250-255.
- 2. Aslan M, Freeman BA. Redox-dependent impairment of
vascular function in sickle cell disease. Free Radical Biology and
Medicine 2007; 43: 1469-1483.
- 3. Steinberg MH. Management of sickle cell disease. New England
Journal of Medicine 1999; 340: 1021-1030.
- 4. Nur E, Biemond BJ, Otten HM, Brandjes DP, Schnog JJ.
CURAMA Study Group. Oxidative stress in sickle cell disease;
pathophysiology and potential implications for disease
management. American Journal of Hematology 2011; 86: 484-
489.
- 5. Parham M, Amini M, Aminorroaya A, Heidarian E. Effect of
zinc supplementation on microalbuminuria in patients with
type 2 diabetes: a double blind, randomized, placebo controlled,
cross-over trial. The Review of Diabetic Studies 2008; 5: 102-
109.
- 6. Yoshikawa T, Toyokuni S, Yamamoto Y, Naito Y. Free Radicals
in Chemistry, Biology and Medicine. UK, London: OICA
International; 2000.
- 7. Lunec J. Free radicals: their involvement in disease processes.
Annals of Clinical Biochemistry 1990; 27: 173-182.
- 8. Prasad AS. Clinical, immunological, anti-inflammatory and
antioxidant roles of zinc. Experimental Gerontology 2008; 43:
370-377.
- 9. Masad A, Hayes L, Tabner BJ, Turnbull S, Cooper LJ et al.
Copper-mediated formation of hydrogen peroxide from the
amylin peptide: a novel mechanism for degeneration of islet cells
in type-2 diabetes mellitus? FEBS Letters 2007; 581: 3489-3493.
- 10. Chirico EN, Pialoux V. Role of oxidative stress in the
pathogenesis of sickle cell disease. IUBMB Life 2012; 64: 72-80.
- 11. Kryukov GV, Castellano S, Novoselov SV, Lobanov AV, Zehtab
O et al. Characterization of mammalian selenoproteomes.
Science 2003; 300: 1439-1443.
- 12. Burk RF. Selenium, an antioxidant nutrient. Nutrition in
Clinical Care 2002; 5: 75-79.
- 13. Canellas CG1, Carvalho SM, Anjos MJ, Lopes RT.
Determination of Cu/Zn and Fe in human serum of patients
with sickle cell anemia using radiation synchrotron. Applied
Radiation and Isotopes 2012; 70: 1277-1280.
- 14. Gaetke LM, Chow CK. Copper toxicity, oxidative stress, and
antioxidant nutrients. Toxicology 2003; 189: 147-163.
- 15. Sarkar A, Dash S, Barik BK, Muttigi MS, Kedage V et al. Copper
and ceruloplasmin levels in relation to total thiols and GST in
type 2 diabetes mellitus patients. Indian Journal of Clinical
Biochemistry 2010; 25: 74-76.
- 16. Kilinç Y, Kümi M, Yilmaz B, Tanyeli A. A comparative study
of zinc and copper values in serum, erythrocytes and urine in
sickle cell homozygotes and heterozygotes. Acta Paediatrica
Scandinavica 1991; 80: 873-874.
- 17. Osredkar J, Sustar N. Copper and zinc, biological role and
significance of copper/zinc imbalance. Journal of Clinical
Toxicology 2011; S3: 001. doi: 10.4172/2161-0495.S3-001.
- 18. Al-Naama LM, Hassan MK, Mehdi JK. Association of
erythrocytes antioxidant enzymes and their cofactors with
markers of oxidative stress in patients with sickle cell anemia.
Qatar Medical Journal 2016; 2015 (2): 14. doi: 10.5339/
qmj.2015.14.
- 19. Bao B, Prasad AS, Beck FWJ, Snell D, Suneja A et al. Zinc
supplementation decreases oxidative stress, incidence of
infection and generation of inflammatory cytokines in sickle
cell disease patients. Translational Research 2008; 152: 67-80.
- 20. Muskiet FA, Muskiet FD, Meiborg G, Schermer JG.
Supplementation of patients with homozygous sickle cell
disease with zinc, alpha-tocopherol, vitamin C, soybean oil,
and fish oil. The American Journal of Clinical Nutrition 1991;
54: 736-744.
- 21. Prasad AS. Zinc deficiency in patients with sickle cell disease.
The American Journal of Clinical Nutrition 2002; 75: 181-182.
- 22. Chasapis CT, Loutsidou AC, Spiliopoulou CA, Stefanidou ME.
Zinc and human health: an update. Archives of Toxicology
2012; 86: 521-534.
- 23. Prasad AS. Zinc in human health: effect of zinc on immune
cells. Molecular Medicine 2008; 14: 353-357.
- 24. Canellas CG, Carvalho SM, Anjos MJ, Lopes RT. Determination
of Cu/Zn and Fe in human serum of patients with sickle
cell anemia using radiation synchrotron. Applied Radiation
and Isotopes 2012; 70: 1277-1280. doi: 10.1016/j.apradiso.
2011.12.028.
- 25. Asadi S, Moradi MN, Khyripour N, Goodarzi MT, Mahmoodi
M. Resveratrol attenuates copper and zinc homeostasis and
ameliorates oxidative stress in type 2 diabetic rats. Biological
Trace Element Research 2017; 177: 132-138.
- 26. Natta CL, Chen LC, Chow CK. Selenium and glutathione
peroxidase levels in sickle cell anemia. Acta Haematologica
1990; 83: 130-132.
- 27. Saxena A, Fayad R, Kaur K, Truman S, Greer J et al. Dietary
selenium protects adiponectin knockout mice against chronic
inflammation induced colon cancer. Cancer Biology and
Therapy 2017; 18: 257-267.
- 28. Bellinger FP, Raman AV, Reeves MA, Berry MJ. Regulation
and function of selenoproteins in human disease. Biochemical
Journal 2009; 422: 11-22.
- 29. Kosaric JV, Cvetkovic DM, Zivanovic MN, Curcic MG,
Seklic DS et al. Antioxidative and antiproliferative
evaluation of 2-(phenylselenomethyl) tetrahydrofuran and
2-(phenylselenomethyl) tetrahydropyran. Journal of BUON:
Official Journal of the Balkan Union of Oncology 2014; 19:
283-290.
- 30. Preoteasa EA, Ionescu I. Fatty-acid peroxidation in relation to
trace elements in serum of patients with homozygous sicklecell anemia and beta-thalassemia: a gas-chromatographic
study. Nutrition 1995; 11 (5 Suppl): 546-450.